Inhibition of coaggregation between Fusobacterium nucleatum and Porphyromonas (Bacteroides) gingivalis by lactose and related sugars

Abstract

The coaggregation of Fusobacterium nucleatum PK1594 and Porphyromonas (Bacteroides) gingivalis PK1924 was inhibited equally well by lactose, N-acetyl-D-galactosamine, and D-galactose, which caused 50% inhibition of coaggregation at 2 mM sugar concentration. Other sugars such as D-galactosamine, D-fucose (6-deoxy-D-galactose), and a-methyland P-methyl-D-galactosides also inhibited coaggregation. Sugar specificity was apparent, since neither L-fucose, L-rhamnose, N-acetyl-D-glucosamine, nor N-acetylneuraminic acid was an inhibitor. Protease treatment of the fusobacterium completely abolished coaggregation, whereas it had no effect on the coaggregating activity of the porphyromonad. Although numerous lactose-inhibitable coaggregating pairs are known to occur among gram-positive bacteria, this report and the accompanying survey (P. E.

Coaggregation between and among human intestinal and oral bacteria

“…Previous studies have shown that coaggregation of oral bacteria is inhibited by specific sugars, often with a structure similar to that of lactose (Kolenbrander & Williams, 1981, 1983McIntire et al, 1982;Kolenbrander & Andersen, 1989;Rickard et al, 2000;Rosen & Sela, 2006). The sugars chosen for this study have previously been used in the characterization of coaggregation between strains derived from the oral cavity (Kolenbrander & Andersen, 1989). The results showed that all of the interactions occurring between oral 13 13 13 13 13 13 13 13 13 13 13 AN/LR 12 12 12 13 12 13 13 13 13 12 and intestinal strains were inhibited to some extent by the addition of individual sugars.…”

Virulence Mechanism of Bacteria in Mixed Infection: Attenuation of Cytokine Levels and Evasion of Polymorphonuclear Leukocyte Phagocytosis

“…Following three washes in phosphate-buffered saline (PBS), the bacterial concentration was spectrophotometrically standardized to OD 650nm = 0.1 for P. gingivalis, corresponding to 10 10 bacteria/mL, 14 and OD 660nm = 0.26 for F. nucleatum, corresponding to 10 9 bacteria/mL. 15 Heat killing was performed at 80°C for 10 minutes. Bacterial viability was determined using blood agar culture.…”

Coaggregation of Porphyromonas gingivalis and Fusobacterium nucleatum PK 1594 is mediated by capsular polysaccharide and lipopolysaccharide

“…Coaggregation between P. gingivalis PK 1924 and F. nucleatum PK 1594 is mediated by a galactoside moiety on the P. gingivalis surface and a lectin on the F. nucleatum, and inhibited by lactose, galactose and related monosaccharides (Kolenbrander & Anderson, 1989;Kinder & Holt, 1993;Shaniztki et al, 1997). Polysaccharides (including lipopolysaccharides (LPS) and lipo-oligosaccharides) of various microorganisms were shown to be involved in their adherence to host cells as well as other bacteria (McIntire et al, 1988;Jaques, 1996;Rosen et al, 2003).…”