Inhibition of Colon Carcinogenesis by Dietary Non-Nutritive Compounds

Tanaka, Takuji; Sugie, Shigeyuki

doi:10.1293/tox.20.215

Cited by 54 publications

(60 citation statements)

References 154 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These chemopreventive agents may target cellular alterations associated with tumorigenesis by anti-oxidant and anti-inflammatory activities and also modulating cell signaling pathways which may lead to inhibition of cell proliferation, cell growth and induction of apoptosis (Gerhauser, 2008). Feeding non-nutritive compounds reduced certain type of cancers in animals via induction of Phase II enzymes, scavenging ROS, suppression of cell proliferation, inhibiting cell invasion (Tanaka et al, 2001;Tanaka and Sugie, 2007) and induction of cytotoxicity (Fresco et al, 2010). In vitro cell culture using the Caco-2 cell line is a well-developed model for studying the effect of phytochemicals on growth inhibition in human colonic adenocarcinoma as part of chemopreventive strategies.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Apoptotic Effects of Sprouted and Non-sprouted Lentil, Green and Yellow Split-peas

Busambwa¹,

Sunkara²,

Diby³

et al. 2015

International J. of Cancer Research

View full text Add to dashboard Cite

Legumes such as lentils, green and yellow split-peas have been reported to provide health benefits against colon cancer due to nutrients and non-nutrient phytochemical compounds present in them. The aim of this study was to investigate the effect of selected legumes in inducing cytotoxicity, apoptosis and/or necrosis in human epithelial colorectal adenocarcinoma cells. Methanolic extracts of sprouted and non-sprouted legumes (Lentils, green peas, yellow peas) were prepared. Caco-2, human colonic carcinoma cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA). Cells were incubated with legume extracts at selected concentrations (1.5, 3, 4.5, 6, 7.5 and 10 mg mLG 1 ) for determination of lactate dehydrogenase (LDH) release (cytotoxicity %). The ability of legume extracts in inducing cell apoptosis was analyzed at selected concentrations of 1.5, 3, 6 and 12 mg mLG 1 by quantifying DNA fragmentation. Morphological changes in cells were observed microscopically. The activity of caspase-3 enzyme was also determined. Results showed that legume extracts induced cytotoxicity in caco-2 cells in a dose-dependent manner. A 20-80% increase in release of LDH was observed in cells incubated with extracts compared to untreated cells. Total fragmented DNA (%) was higher in cells treated with sprouted legume extracts compared to their non-sprouted extracts. More than 40% of total DNA fragmentation was observed in cells treated with sprouted legumes. Caspase-3 activity was highest in cells incubated with non-sprouted yellow split peas at 6 mg mLG 1 . Specific morphological changes related to apoptosis such as: surface blebbing, formation of apoptotic bodies, blisters and echinoid spikes and cell membrane destabilization were observed in cells incubated with extracts. These results suggested the cytotoxic and apoptosis inducing potential of legume extracts. Bioactive components in these selected legumes may be used as chemopreventive agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Apoptotic Effects of Sprouted and Non-sprouted Lentil, Green and Yellow Split-peas

Busambwa¹,

Sunkara²,

Diby³

et al. 2015

International J. of Cancer Research

View full text Add to dashboard Cite

show abstract

“…CRC in humans is in many cases related to feeding habits: dietary substances such as saturated fats and ω-6 fatty acids favor the development of dysplasia in the crypts of the colonic mucosa and can promote carcinogenesis, while diets rich in polyunsaturated fat, -3 and ω-9 fatty acids, insoluble fibers, certain vitamins (D, A, C, E and β-carotene), minerals (calcium, selenium) and antioxidants (folates, flavonoids) may reduce the incidence [16][17][18] . It is estimated that CRC is causally associated with environmental factors, especially diet-related factors, in 80% of cases 19 .…”

Section: Discussionmentioning

confidence: 99%

Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa

Burlamaqui¹,

Dornelas²,

Júnior³

et al. 2012

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE: To determine whether a hypercaloric and hyperlipidic diet enriched with polyunsaturated fatty acids influences the formation of aberrant crypt foci (ACF) in colonic mucosa of Wistar rats treated with azoxymethane (AOM). METHODS: At eight weeks of life, the rats were assigned to four groups: Group I―standard diet (STD) not treated with AOM; Group II―hypercaloric and hyperlipidic diet (FED), not treated with AOM; Group III―STD, treated with AOM; Group IV―FED, treated with AOM. At 16 weeks, the animals were injected intraperitoneal with 0.9% saline solution (Group I and II) or AOM at 15mg/Kg (Groups III and IV) once a week for two weeks. Fifteen weeks later, the animals were euthanized. RESULTS: FED promoted weight gain in Groups II and IV compared to Groups I and III, respectively. The groups did not differ with regard to the total number of ACF. The Chi-square test revealed no predominance of the presence of foci with <4 crypts. However, foci with ≥5 crypts were proportionally more prevalent in Group III than in Group IV (p=0.043). CONCLUSION: The administration of polyunsaturated fatty acids did not interfere with the formation of aberrant crypt foci, but reduced ACF multiplicity, exercising an attenuating effect on carcinogenesis.

show abstract

“…We suspected that hesperidin in CUSM may therefore be responsible for the inhibition of preneoplasia development in male db/db mice, because this chemical can inhibit chemically-induced colon carcinogenesis in rodents. 19 An association between diabetes and cancer was suggested over 100 years ago. 42 The increased incidence of CRC in diabetic patients, mainly in those with type 2 diabetes, has been supported by a recent prospective, population-based cohort, case-control and meta-analysis studies.…”

Section: Discussionmentioning

confidence: 99%

“…19 However, few studies have so far been performed on the preventive effect of food components on obesity/diabetes-related colon carcinogenesis. 20,21 We recently have made the citrus unshiu segment membranes (CUSMs) that are rich in soluble and insoluble fiber and separate the juice vesiculates, from Satsuma mandarin (Citrus unshiu Marc.).…”

mentioning

confidence: 99%

Diet supplemented with citrus unshiu segment membrane suppresses chemically induced colonic preneoplastic lesions and fatty liver in male db/db mice

Suzuki

Kohno

Yasui

et al. 2006

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

The modulatory effects of dietary citrus unshiu segment membrane (CUSM) on the occurrence of aberrant crypt foci (ACF) and b-catenin accumulated crypts (BCACs) were determined in male C57BL/KsJ-db/db (db/db) mice initiated with azoxymethane (AOM). Male db/db, db/þ and þ/þ mice were given 5 weekly subcutaneous injections of AOM (15 mg/kg body weight), and then they were fed the diet containing 0.02%, 0.1% or 0.5% CUSM for 7 weeks. At Week 12, a significant increase in the numbers of ACF and BCAC was noted in the db/db mice in comparison with the db/ þ and þ/þ mice. Feeding with CUSM caused reduction in the frequency of ACF in all genotypes of mice and the potency was high in order of the db/db mice, db/þ mice and þ/þ mice. The number of BCACs was also reduced by feeding with CUSM, thus resulting in a 28-61% reduction in the db/db mice, possibly due to suppression of cell proliferation activity in the lesions by feeding with CUSM-containing diet. Clinical chemistry revealed a low serum level of triglyceride in mice fed CUSM. In addition, CUSM feeding inhibited fatty metamorphosis and fibrosis in the liver of db/db mice. Our findings show that CUSM in the diet has a chemopreventive ability against the early phase of AOM-induced colon carcinogenesis in the db/db as well as db/þ and þ/þ mice, indicating potential use of CUSM in cancer chemoprevention in obese people. ' 2006 Wiley-Liss, Inc.Key words: citrus unshiu segment membrane; ACF; BCAC; colon carcinogenesis; db/db mice The modern Western lifestyle, including a high caloric intake, high-fat diets and physical inactivity, results in a positive energy balance, diabetes and obesity. These lifestyle patterns might also be risk factors for the development of colorectal cancer (CRC), 1 which is one of the major causes of morbidity and mortality in the Western world. 2 This malignancy has also increased in Asia owing to the changes in lifestyle, such as the dietary habit of increased meat consumption. 2,3 Several prospective and case-control studies have addressed the relationship between obesity/diabetes and CRC. 1,4,5 C57BL/KsJ-db/db (db/db) mice are used as a genetically altered animal model with genotypes of obesity and diabetes mellitus. 6 A disruption of the leptin receptor (Ob-R) gene in these mice leads to an over-expression of leptin in the adipose tissue and a concomitantly high serum concentration of leptin. 7,8 The synthesis of leptin in adipocytes is influenced by insulin, 9 tumor necrosis factor-a, 10 glucocorticoids, 11 reproductive hormones 12 and prostaglandins 13 that may be involved in the neoplastic processes. 14 In addition, leptin can act as a growth factor in colonic epithelial cells 15 while modulating the proliferation of colonic cryptal cells. 16 In contrast, more leptin in the blood clearly decreased colon carcinogenesis in 3 different animal models. 17,18 Thus, leptin might be one of the biological factors involved in the development of CRC associated with obesity/diabetes. The db/db mouse, therefore, is a very useful model for elucidating ...

show abstract

Inhibition of Colon Carcinogenesis by Dietary Non-Nutritive Compounds

Cited by 54 publications

References 154 publications

Cytotoxic and Apoptotic Effects of Sprouted and Non-sprouted Lentil, Green and Yellow Split-peas

Cytotoxic and Apoptotic Effects of Sprouted and Non-sprouted Lentil, Green and Yellow Split-peas

Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa

Diet supplemented with citrus unshiu segment membrane suppresses chemically induced colonic preneoplastic lesions and fatty liver in male db/db mice

Contact Info

Product

Resources

About