Inhibition of Colony-spreading Activity of Staphylococcus aureus by Secretion of δ-Hemolysin

Omae, Yosuke; Sekimizu, Kazuhisa; Kaito, Chikara

doi:10.1074/jbc.m112.357848

Cited by 20 publications

(26 citation statements)

References 41 publications

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“…A DNA fragment containing the agrA gene was amplified by polymerase chain reaction (PCR) using oligonucleotide primer pairs agrA-HisC-F and agrA-HisC-R and pW as a template [39]. The amplified DNA fragment was phosphorylated by T4 polynucleotide kinase and self-ligated, resulting in pW-agrAHis.…”

Section: Methodsmentioning

confidence: 99%

Mobile Genetic Element SCCmec-encoded psm-mec RNA Suppresses Translation of agrA and Attenuates MRSA Virulence

et al. 2013

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Community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) is a socially problematic pathogen that infects healthy individuals, causing severe disease. CA-MRSA is more virulent than hospital associated-MRSA (HA-MRSA). The underlying mechanism for the high virulence of CA-MRSA is not known. The transcription product of the psm-mec gene, located in the mobile genetic element SCCmec of HA-MRSA, but not CA-MRSA, suppresses the expression of phenol-soluble modulin α (PSMα), a cytolytic toxin of S. aureus. Here we report that psm-mec RNA inhibits translation of the agrA gene encoding a positive transcription factor for the PSMα gene via specific binding to agrA mRNA. Furthermore, 25% of 325 clinical MRSA isolates had a mutation in the psm-mec promoter that attenuated transcription, and 9% of the strains had no psm-mec. In most of these psm-mec-mutated or psm-mec-deleted HA-MRSAs, PSMα expression was increased compared with strains carrying intact psm-mec, and some mutated strains produced high amounts of PSMα comparable with that of CA-MRSA. Deletion of psm-mec from HA-MRSA strains carrying intact psm-mec increased the expression of AgrA protein and PSMα, and virulence in mice. Thus, psm-mec RNA suppresses MRSA virulence via inhibition of agrA translation and the absence of psm-mec function in CA-MRSA causes its high virulence property.

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Section: Methodsmentioning

confidence: 99%

Mobile Genetic Element SCCmec-encoded psm-mec RNA Suppresses Translation of agrA and Attenuates MRSA Virulence

et al. 2013

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“…The expression of agr locus also provides a simultaneous impact on colony spreading and virulent determinants and are regulated by the environmental cues [25]. The colony spreading in S. aureus is negatively regulated by the d-hemolysin and the experimental data also demonstrated that hld mutant strains confirm the prominent colony spreading than the parental level [26]. An aminoswapping in vitro analysis revealed that virulence gene expression is suppressed by the phenylalanine whereas the hemolytic and protease activity are being repressed by the aspartic acid [27].…”

Section: Agr: the Global Regulatormentioning

confidence: 95%

Exploration of Modulated Genetic Circuits Governing Virulence Determinants in Staphylococcus aureus

Arya

Princy

2015

Indian J Microbiol

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The expression of virulence genes in the human pathogen Staphylococcus aureus is strongly influenced by the multiple global regulators. The signal transduction cascade of these global regulators is accountable for recognizing and integrating the environmental cues to regulate the virulence regulon. While the production of virulent factors by individual global regulators are comparatively straightforward to define, auto-regulation of these global regulators and their impact on other regulators is more complex process. There are several reports on the production of virulent factors that are precisely regulated by switching processes of multiple global regulators including some prominent accessory regulators such as agr, sae and sar which allows S. aureus to coordinate the gene expression, and thus, provide organism an ability to act collectively. This review implicates the mechanisms involved in the global regulation of various virulence factors along with a comprehensive discussion on the differences between these signal transduction systems, their auto-induction and, coordination of classical and some comparatively new bacterial signal transduction systems.

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“…The colony spreading test procedure was carried out based on the studies of Kaito and Sekimizu (2006) and Omae et al (2012). Mixing TSB supplemented with 0.24% agar with Javanese citronella oil was done to obtain bacteriostatic and bactericidal concentrations.…”

Section: Anticolony Spreading Mrsa Testmentioning

confidence: 99%

Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA)

Hidayah

Wasito

Debora

et al. 2019

FMI

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Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has been resistant to various types of antibiotics, so it is not easy to be treated with antibiotics and needs other solutions. Javanese citronella oil distilled from the Cymbopogon nardus plant is proven to function as an antibacterial agent (bacteriostatic and bactericidal), fungicide and repellent. This study aimed to prove that there is a positive correlation between bacteriostatic and bactericidal effects with antibiofilm and anticolony spreading from Javanese citronella oil on MRSA. The intended antibiofilm is a barrier to biofilm formation and eradication. Bacteriostatic and antibiofilm effects were tested using microtiter plates assay, bactericidal effect test with subculture into the media and anticolony spreading effect test with spot inoculation in Tryptic Soy Broth media supplemented with 0.24% agar. The bacteriostatic effect test data were analyzed using paired t-test, bactericidal effect using the Friedman test, antibiofilm effect test using Kruskall-Wallis and the results of all the tests correlated using Pearson and Spearman correlation. The statistical significance used was p<0.05. The results showed that Javanese citronella oil had a bacteriostatic concentration of 0.02% (v/v) and bactericidal concentration of 0.78% (v/v). The Pearson correlation test showed that there was a negative correlation between bacteriostatic and bactericidal effects on biofilm formation with r=-0.956 (p=0.000), but the correlation was positive for biofilm eradication with r=0.918 (p=0.000) and anticolony spreading with r=1.000 (p=0.000).

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Inhibition of Colony-spreading Activity of Staphylococcus aureus by Secretion of δ-Hemolysin

Cited by 20 publications

References 41 publications

Mobile Genetic Element SCCmec-encoded psm-mec RNA Suppresses Translation of agrA and Attenuates MRSA Virulence

Mobile Genetic Element SCCmec-encoded psm-mec RNA Suppresses Translation of agrA and Attenuates MRSA Virulence

Exploration of Modulated Genetic Circuits Governing Virulence Determinants in Staphylococcus aureus

Correlation between the Bacteriostatic and Bactericide Effect with Antibiofilm and Anticolony Spreading from Javanese Citronella Oil on Methicillin-Resistant Staphylococcus aureus (MRSA)

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