Inhibition of cyclooxygenase-2 expression and prostaglandin E2 production in chondrocytes by avocado soybean unsaponifiables and epigallocatechin gallate

Heinecke, L.F.; Grzanna, M.W.; Au, A.Y.; Mochal, C.A.; Rashmir‐Raven, Ann M.; Frondoza, Carmelita G.

doi:10.1016/j.joca.2009.08.015

Cited by 65 publications

(68 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although all cells used in this study were isolated from degenerated discs, the difference in the expression of studied inflammation-related genes between the untreated control group and the EGCG-treated group is not significant, because basal expression of these genes decreases when cells are transferred to in vitro conditions (data not shown). While this is the first IVD-related study, protective effects of EGCG and green tea extract have been reported in inflammatory arthritis (Shen et al, 2012), where EGCG was shown to block IL-1β-induced expression of IL-6, IL-7, IL-8, IL-1β, TNF-α (Akhtar and Haqqi, 2011), MMP1 and MMP13 (Ahmed et al, 2004), iNOS (Singh et al, 2002) and COX-2 (Heinecke et al, 2010), and these effects were linked mostly to NF-κB and JNK signalling pathways Akhtar and Haqqi, 2011;Rasheed et al, 2009;Singh et al, 2002;Singh et al, 2003). In order to determine the underlying mechanisms in the IVD, potential pathways were identified from the literature Akhtar and Haqqi, 2011;Rasheed et al, 2009;Singh et al, 2002;Singh et al, 2003) and the effects of EGCG on IRAK-1 degradation as well as NFkB and MAPK activity in IVD cells were analysed.…”

Section: Discussionmentioning

confidence: 85%

Epigallocatechin 3-gallate suppresses interleukin-1β-induced inflammatory responses in intervertebral disc cells in vitro and reduces radiculopathic pain in rats

Krupková¹,

Sekiguchi²,

Klasen³

et al. 2014

eCM

View full text Add to dashboard Cite

Intervertebral disc (IVD) disease, which is characterised by age-related changes in the adult disc, is the most common cause of disc failure and low back pain. The purpose of this study was to analyse the potential of the biologically active polyphenol epigallocatechin 3-gallate (EGCG) for the treatment of painful IVD disease by identifying and explaining its anti-inflammatory and anti-catabolic activity. Human IVD cells were isolated from patients undergoing surgery due to degenerative disc disease (n = 34) and cultured in 2D or 3D. An inflammatory response was activated by IL-1, EGCG was added, and the expression/activity of inflammatory mediators and pathways was measured by qRT-PCR, western blotting, ELISA, immunofluorescence and transcription factor assay. The small molecule inhibitor SB203580 was used to investigate the involvement of the p38 pathway in the observed effects. The analgesic properties of EGCG were analysed by the von Frey filament test in Sprague-Dawley rats (n = 60). EGCG significantly inhibited the expression of pro-inflammatory mediators and matrix metalloproteinases in vitro, as well as radiculopathic pain in vivo, most probably by modulation of the activity of IRAK-1 and its downstream effectors p38, JNK and NF-B. AbstractIntervertebral disc (IVD) disease, which is characterised by age-related changes in the adult disc, is the most common cause of disc failure and low back pain. The purpose of this study was to analyse the potential of the biologically active polyphenol epigallocatechin 3-gallate (EGCG) for the treatment of painful IVD disease by identifying and explaining its anti-inflammatory and anti-catabolic activity. Human IVD cells were isolated from patients undergoing surgery due to degenerative disc disease (n = 34) and cultured in 2D or 3D. An inflammatory response was activated by IL-1β, EGCG was added, and the expression/activity of inflammatory mediators and pathways was measured by qRT-PCR, western blotting, ELISA, immunofluorescence and transcription factor assay. The small molecule inhibitor SB203580 was used to investigate the involvement of the p38 pathway in the observed effects. The analgesic properties of EGCG were analysed by the von Frey filament test in SpragueDawley rats (n = 60). EGCG significantly inhibited the expression of pro-inflammatory mediators and matrix metalloproteinases in vitro, as well as radiculopathic pain in vivo, most probably by modulation of the activity of IRAK-1 and its downstream effectors p38, JNK and NF-κB.

show abstract

Section: Discussionmentioning

confidence: 85%

Epigallocatechin 3-gallate suppresses interleukin-1β-induced inflammatory responses in intervertebral disc cells in vitro and reduces radiculopathic pain in rats

Krupková¹,

Sekiguchi²,

Klasen³

et al. 2014

eCM

View full text Add to dashboard Cite

show abstract

“…Cells were seeded in flasks, and allowed to grow to confluency in control media consisting of Dulbeccos' Modified Eagle's basal medium (Sigma; St. Louis, MO, USA) supplemented with 10% v/v fetal bovine serum (Gemini Bio-Products; Woodland, CA, USA), 300 mg/ml L-glutamine (Sigma), 30 mg/ml antibiotic/antimycotic (Sigma), and 3.7 g/L sodium bicarbonate (Sigma), pH 7.4at 37°C, 5% CO 2 [9]. Chondrocytes were used at passage 2 -4.…”

Section: Treatment Designmentioning

confidence: 99%

“…Chondrocyte phenotype was characterized by immunofluorescence staining for collagen and aggrecan [9]. Chondrocytes were seeded at 1 × 10 4 cells/well on Slides were washed 3 times with PBS and nuclei were stained with DAPI (Gibco Invitrogen) for 30 minutes as described above.…”

Section: Collagen and Aggrecan Immunofluorescencementioning

confidence: 99%

“…Because of their common structural, functional and cellular characteristics, horses have been used as an in vivo model for studying human OA. Equine joint tissues such as cartilage and their constituent chondrocytes have also been beneficial as models for in vitro studies [7] [8] [9] [10] [11].…”

Section: Introductionmentioning

confidence: 99%

“…ASU, GLU and CS have been documented to reduce inflammation in vitro [9] [10] [11], [31]- [38], and in vivo [39]- [46]. These agents have been reported to ameliorate OA in man and animals with minimal adverse side effects [39]- [46].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chondrocyte Production of Pro-Inflammatory Chemokine MCP-1 (CCL-2) and Prostaglandin E-2 Is Inhibited by Avocado/Soybean Unsaponifiables, Glucosamine, Chondroitin Sulfate Combination

Secor¹,

Grzanna²,

Rashmir‐Raven³

et al. 2018

View full text Add to dashboard Cite

Osteoarthritis (OA) is a chronic, painful disease affecting articulating joints in man and animals. It is characterized by cartilage breakdown, bone remodeling, osteophyte formation and joint inflammation. Currently used non-steroidal anti-inflammatory drugs for the management of OA are known to have deleterious side effects. To address the need for alternatives, we evaluated the anti-inflammatory effects of a combination of avocado/soybean unsaponifiables (ASU), glucosamine (GLU) and chondroitin sulfate (CS) by measuring chemokine MCP-1 (monocyte chemoattractant protein 1, CCL2) and prostaglandin E-2 (PGE 2 ) in stimulated chondrocytes. As the only cellular constituents of cartilage, chondrocytes are the source of pro-inflammatory mediators that play critical roles in the pathogenesis of OA. Chondrocytes were incubated: with: 1) control media, 2) [ASU + GLU + CS] combination, or 3) Phenylbutazone (PBZ) for 24 hours. Cells were next stimulated with IL-1β or LPS for another 24 hrs. MCP-1 and PGE 2 from supernatants were quantitated by immunoassay. Another set of chondrocytes seeded in chamber slides were stimulated with IL-1β for 1 hour and then immunostained for NF-κB. Chondrocytes stimulated with IL-1β or LPS significantly increased MCP-1 and PGE 2 production which were significantly reduced after treatment with [ASU + GLU + CS]. In contrast, PBZ significantly reduced PGE 2 but not MCP-1 production. IL-1β stimulation induced nuclear translocation of NF-κB, which The present study provides evidence that the production of MCP-1 by chondrocytes can be inhibited by the combination of [ASU + GLU + CS] but not by PBZ. In contrast, PGE 2 production was inhibited by either treatment suggesting that the production of MCP-1 and PGE 2 could be independently regulated. The finding of distinct effects of [ASU + GLU + CS] on MCP-1 and PGE 2 synthesis supports a scientific rationale for a multimodal treatment approach in the management of OA.

show abstract

Potential Health Benefits of Avocados

Ingram

Elks

Davenport³

et al. 2013

Bioactives in Fruit

View full text Add to dashboard Cite

Inhibition of cyclooxygenase-2 expression and prostaglandin E2 production in chondrocytes by avocado soybean unsaponifiables and epigallocatechin gallate

Abstract: The present study demonstrates that the anti-inflammatory activity of ASU and EGCG is potentiated when used in combination. This combination may offer an attractive supplement or alternative to non-steroidal anti-inflammatory drugs (NSAIDs) in the management of osteoarthritis.

Cited by 65 publications

References 58 publications

Epigallocatechin 3-gallate suppresses interleukin-1β-induced inflammatory responses in intervertebral disc cells in vitro and reduces radiculopathic pain in rats

Epigallocatechin 3-gallate suppresses interleukin-1β-induced inflammatory responses in intervertebral disc cells in vitro and reduces radiculopathic pain in rats

Chondrocyte Production of Pro-Inflammatory Chemokine MCP-1 (CCL-2) and Prostaglandin E-2 Is Inhibited by Avocado/Soybean Unsaponifiables, Glucosamine, Chondroitin Sulfate Combination

Potential Health Benefits of Avocados

Contact Info

Product

Resources

About