Inhibition of denitrification by uncouplers of oxidative phosphorylation

Walter, Bert; Sidransky, Ellen; Kristjánsson, Jakob K.; Hollocher, Thomas C.

doi:10.1021/bi00608a015

Cited by 30 publications

(22 citation statements)

References 25 publications

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“…The amount of endogenous substrate is however sufficient to drive steady-state respiration at an appreciable rate. Analogous results have been reported for Pseudomonas denitrjf icans [34].…”

Section: Growth Eyperiments In Butch and Clzemostat Culturesupporting

confidence: 88%

Anaerobic Respiration and Energy Conservation inParacoccus denitrficans

Meijer

Zwaan

Stouthamer

et al. 1979

European Journal of Biochemistry

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1. Electron paramagnetic resonance spectra at 8 -60 K of NADH-reduced membrane particles prepared from Paracoccus denitrificans grown anaerobically with nitrate as terminal electron acceptor show the presence of iron-sulfur centers 1-4 in the NADH-ubiquinone segment of the respiratory chain. In addition resonance lines at g = 2.058, g = 1.953 and g = 1.88 are detectable in the spectra of succinate-reduced membranes at 15 K, which are attributed to the iron-sulfurcontaining nitrate reductase.2. Sulphate-limited growth under anaerobic conditions does not affect the iron-sulfur pattern of NADH dehydrogenase or nitrate reductase. Furthermore respiratory chain-linked electron transport and its inhibition by rotenone are not influenced. These results contrast those observed for sulphate-limited growth of P. denitrificans under aerobic conditions [Eur. J . Biochem. (1977) 81, 3. Proton translocation studies of whole cells indicate that nitrite increases the proton conductance of the cytoplasmic membrane, resulting in a collapse of the proton gradient across the membrane. Nitrite accumulates under anaerobic growth conditions with nitrate as terminal electron acceptor; the extent of accumulation depends on the specific growth conditions. Thus the low efficiencies of respiratory chain-linked energy conservation observed during nitrate respiration [Arch. Micvohiol. (1977) 112, 17-23] can be explained by the uncoupling action of nitrite.

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Section: Growth Eyperiments In Butch and Clzemostat Culturesupporting

confidence: 88%

Anaerobic Respiration and Energy Conservation inParacoccus denitrficans

Meijer

Zwaan

Stouthamer

et al. 1979

European Journal of Biochemistry

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“…However, data from the ferrozine and cytochrome assays demonstrate that these compounds act as inhibitors of Fe(III) reduction as well as nitrite and fumarate reduction, probably by interaction with a component of the respiratory chain. Inhibition of denitrification reactions mediated by Pseudomonas denitrificans and Pseudomonas aeruginosa with FCCP and CCCP has previously been noted (Walter et al 1978).…”

Section: Discussionmentioning

confidence: 90%

The influence of chelating agents upon the dissimilatory reduction of Fe(III) by Shewanella putrefaciens

et al. 1995

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The ability of S. putrefaciens to reduce Fe(III) complexed by a variety of ligands has been investigated. All of the ligands tested caused the cation to be more susceptible to reduction by harvested whole cells than when uncomplexed, although some complexes were more readily reduced than others. Monitoring rates of reduction by a ferrozine assay for Fe(II) formation proved inadequate using Fe(III) ligands giving Fe(II) complexes of low kinetic lability (e.g. EDTA). A more suitable assay for Fe(III) reduction in the presence of such ligands proved to be the observation of associated cytochrome oxidation and re-reduction. Where possible, an assay for Fe(III) reduction based upon the disappearance of Fe(III) complex was also employed. Reduction of all Fe(III) complexes tested was totally inhibited by the presence of 02, partially inhibited by HQNO and slower in the absence of a physiological electron donor. Upon cell fractionation, Fe(III) reductase activity was detected exclusively in the membranes. Using different physiological electron donors in assays on membranes, relative reduction rates of Fe(III) complexes complemented the data from whole cells. The differences in susceptibility to reduction of the various complexes are discussed, as is evidence for the respiratory nature of the reduction.

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“…Analogous experiments with those of St. John and Hollocher (1977) were carried out by Walter et al (1978) using P. denitrificans (ATCC 13867) that was grown in a yeast extract medium at 30°C with nitrous oxide, nitrite, and nitrate as electron acceptors. They found indeed that the reduction of nitrous oxide was faster than that of nitrite.…”

Section: July/august 1996mentioning

confidence: 99%

Kinetics of denitrification by Pseudomonas denitrificans under growth conditions limited by carbon and/or nitrate or nitrite

Kornaros¹,

Zafiri²,

Lyberatos³

1996

Water Environment Research

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Kinetic experiments were carried out for a pure culture of Pseudomonas denitrificans to develop a mathematical model that adequately describes denitrification under both carbon and nitrate or nitrite electron acceptor limited conditions. Denitrification was assumed to occur by the consecutive reduction of nitrates to nitrites and then to nitrogen gas without accumulation of intermediate gaseous products. The denitrification kinetics, assuming they are associated with cell growth, could be very well described by using Monod expressions with double substrate limitation (terminal electron acceptor and organic carbon). The kinetic parameters of the model were evaluated exclusively from batch experimental data. The proposed model is able to predict accurately the biomass growth, the nitrate and nitrite reduction rates, as well as the accumulation of nitrite-nitrogen as an intermediate, due to nitrate inhibition on the nitrite reduction rate. Water Environ. Res., 68, 934 (1996).

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Inhibition of denitrification by uncouplers of oxidative phosphorylation

Cited by 30 publications

References 25 publications

Anaerobic Respiration and Energy Conservation inParacoccus denitrficans

Anaerobic Respiration and Energy Conservation inParacoccus denitrficans

The influence of chelating agents upon the dissimilatory reduction of Fe(III) by Shewanella putrefaciens

Kinetics of denitrification by Pseudomonas denitrificans under growth conditions limited by carbon and/or nitrate or nitrite

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