Inhibition of DNA synthesis in mammalian cells by actidione

Bennett, L. Lee; Smithers, Donald; Ward, Caroline T.

doi:10.1016/0926-6550(64)90047-7

Cited by 49 publications

(40 citation statements)

References 11 publications

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“…Vol. 55, 1975 5.50 (1) 0.13 (2) (1) (5). Cursory consideration of this result seems to suggest that CH effects on the cell could be mediated only through inhibition of protein synthesis.…”

Section: Discussionmentioning

confidence: 89%

“…This conclusion is in contrast to that of a number of other investigations which were described in the introduction. Instead of inhibiting RNA synthesis, interference with protein synthesis in ts mutants or by amino acid starvation (26) (2) …”

Section: Discussionmentioning

confidence: 99%

“…It inhibits DNA synthesis (2,4). It does not inhibit RNA synthesis in the alga, Acetabiularia mnediterranea (3), but this organism is an exception for it inhibits the synthesis of RNA in Neurospora crassa (34), yeasts (6,22,32), Dictyostelium discoideum (16), rat (11,29), monkey (8), and man (17,36 terferes with other cellular functions including respiration (9,21), ion absorption (21), amino acid uptake (7,15,33), and the interconversion of pyrimidine nucleotides (31).…”

mentioning

confidence: 99%

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Cycloheximide Is Not a Specific Inhibitor of Protein Synthesis in Vivo

McMahon

1975

Plant Physiol.

116

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“…Vol. 55, 1975 5.50 (1) 0.13 (2) (1) (5). Cursory consideration of this result seems to suggest that CH effects on the cell could be mediated only through inhibition of protein synthesis.…”

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Cycloheximide Is Not a Specific Inhibitor of Protein Synthesis in Vivo

McMahon

1975

Plant Physiol.

116

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“…(16). In some systems, high CH concentrations may elicit other effects, including (a) uncoupling of respiration (3,21), (b) inhibition in nongreen storage tissue of ion and organic molecule uptake (3), and (c) interference with nucleotide metabolism (1,5,7,9). However, protein synthesis in Onoclea is blocked by CH (Table II) whereas the uncoupling of respiration could not be demonstrated (Table III and Fig.…”

mentioning

confidence: 74%

Photocontrol of the Germination of Onoclea Spores

Towill¹,

Ikuma²

1975

Plant Physiol.

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Light has been implicated in the control of plant growth and development by regulation of enzyme synthesis, or enzyme activity, or both (14,22). In view of the observations that protein synthesis is required for the early phase of dark germination of spores of the bracken fern (11,12) and that one of the early events after the induction of germination by light in fern spores is hydrolysis of storage products (6, 11), it would appear that protein synthesis may play an important role in the control of photoinduced germination of fern spores.Investigations with a nitrogen atmosphere (18) by anaerobiosis, the recovery process is slow, thus implicating the synthesis of a substance or substances (possibly enzymes) required for the germination process. Edwards and Miller (2) also showed that during the first 8 to 10 hr of illumination germination is inhibited by ethylene, suggesting the involvement of nucleic acid synthesis during this period of germination.Involvement of protein and RNA synthesis in the germination of Onoclea spores was investigated using inhibitors. As indicated in the results, of the five inhibitors tested, cycloheximide inhibited germination completely in 2 hr, and this inhibition was found to be reversed after the removal of the inhibitor. This paper examines the requirement for protein synthesis during the pre-and postinduction phases of Onoclea spore germination by means of the temporary application of CH.MATERIALS AND METHODS Plant Material. Spores used in experiments on the effect of CH2 on germination were from the same batch employed in the previously reported experiments on anaerobiosis (18). Spores of this batch required 6 hr of dark presoaking to develop maximal photosensitivity, and gave the final germination of 65.1 + 2.8%. For these experiments, sterilization of the spores was not necessary, because possible fungal and bacterial contamination hardly altered the results.A second batch of spores was employed for the experiments on incorporation of the radioactivity. These spores were harvested from sporophylls collected in March 1972 from the same location as reported previously (17) and required 24 hr of dark presoaking to develop maximal sensitivity to irradiation. The final germination for this batch was 82.8 + 3.0%. Maximal inhibition of germination by CH was 90 to 97% for both batches of spores. For the incorporation experiments, spores were surface-sterilized as follows: a small spatulaful of spores (2-4 mg) was treated first with 5 ml of 2% (v/v) Clorox (prepared from a commercial solution) in a 5-cm Petri dish and allowed to stand with occasional agitation for 5 min. They were then treated with 5 ml of 0.1% (w/v) HgCl2 for 2 min and washed 4 times with sterile distilled H20. Aftre the final washing, 10 ml of sterile distilled H20 were added to the Petri dishes. To check for bacterial and fungal contamination, samples of spores were plated onto bacto-nutrient agar after surface sterilization. Experimental runs that showed contamination by this testing procedure were disca...

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“…Frior publications showed that actidione inhibited protein synthesis in a number of organisms by preventing the transfer of activated amino acids to the growing peptide chain (24,27). This compound also inhibited DNA synthesis in vivo without markedly affecting the in vitro activity of DNA polymerase (1). In the experiments which follow, it is assumed that the primary effect of actidione is on protein synthesis, and the observed effects on DNA replication are regarded as secondary consequences of the inhibition of protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

LIMITED DNA SYNTHESIS IN THE ABSENCE OF PROTEIN SYNTHESIS IN PHYSARUM POLYCEPHALUM

Cummins

Rusch

1966

The Journal of Cell Biology

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Actidione (cycloheximide), an antibiotic inhibitor of protein synthesis, blocked the incorporation of leucine and lysine during the S phase of Physarum polycephalum. Actidione added during the early prophase period in which mitosis is blocked totally inhibited the initiation of DNA synthesis. Actidione treatment in late prophase, which permitted mitosis in the absence of protein synthesis, permitted initiation of a round of DNA replication making up between 20 and 30 % of the unreplicated nuclear DNA. Actidione treatment during the S phase permitted a round of replication similar to the effect at the beginning of S. The DNA synthesized in the presence of actidione was replicated semiconservatively and was stable through at least the mitosis following antibiotic removal. Experiments in which fluorodeoxyuridine inhibition was followed by thymidine reversal in the presence of actidione suggest that the early rounds of DNA replication must be completed before later rounds are initiated.

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Inhibition of DNA synthesis in mammalian cells by actidione

Cited by 49 publications

References 11 publications

Cycloheximide Is Not a Specific Inhibitor of Protein Synthesis in Vivo

Cycloheximide Is Not a Specific Inhibitor of Protein Synthesis in Vivo

Photocontrol of the Germination of Onoclea Spores

LIMITED DNA SYNTHESIS IN THE ABSENCE OF PROTEIN SYNTHESIS IN PHYSARUM POLYCEPHALUM

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