Inhibition of E. coli DNA polymerase I by 1,10-phenanthroline

D'Aurora, Vito; Stern, Andrew M.; Sigman, David S.

doi:10.1016/0006-291x(77)91236-0

Cited by 37 publications

(15 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The formation of a noncovalent complex analogous to a Michaelis complex in the cleavage of poly(dA-dT) has already been inferred based on (i) the reactivity of the cuprous complex of 1,10-phenanthroline relative to the reactivities of the cuprous complexes of bipyridine and 2,2',2"-terpyridine (31,32), (ii) the inhibition of the cleavage by intercalating agents (14), and (iii) the lack of reactivity of noncomplement-agents (14), and (iii) the lack of reactivity of noncomplementing single-stranded DNAs such as poly(dT) (14). Although it is tempting to assume that the complex between a B-DNA and (OP)2Cu+ involves intercalation because of the structural similarity of the ligand to ethidium bromide, no firm evidence for this binding mode, as opposed to interaction with the major or minor groove, is available.…”

Section: Discussionmentioning

confidence: 99%

Secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.

Pope¹,

Sigman²

1984

Proc. Natl. Acad. Sci. U.S.A.

110

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.

Pope¹,

Sigman²

1984

Proc. Natl. Acad. Sci. U.S.A.

110

View full text Add to dashboard Cite

show abstract

“…The Cu(1) complex is then thought to inhibit the polymerase by cleaving the template in an oxygen-dependent reaction, where the products are effective inhibitors of the enzymes. This scheme is based on: (I) the requirement for thiols in the assay mixture before the inhibition by 1 ,lo-phenanthroline can be expressed (D'Aurora et al, 1977(D'Aurora et al, , 1978; (2) the dependence of the cleavage reaction on the concentration of oxygen and its blockage by catalase (Sigman et al, 1979); (3) the reversal of the inhibition by the chelating agent 2,9-dimethyl-l , 10-phenanthroline, which is considered specific for Cu(1) (D'Aurora et al., 1978).…”

Section: Properties Of the Metal Centersmentioning

confidence: 99%

The Metallobiochemistry of Zinc Enzymes

Vallée

Galdes

1984

Advances in Enzymology - And Related Areas of Molecular Biology

175

View full text Add to dashboard Cite

The honour and opportunity of being invited to address you on the occasion of Professor Houtman's retirement is very much appreciated. However, having met him yesterday, I am convinced that I am not here to help celebrate his retirement as much as to initiate his new career.Just like careers, the role of metals in biology can be viewed from multiple points of view. In the past, metals have variously been thought to be beneficial, toxic or to serve no purpose at all in biology. It is not very long ago that documentation for the validity of all th ree perceptions seemed to coexist, and that they had equally vigorous advocates; but by now the hypothesis that they serve no biological purpose has faded into oblivion. This dilemma owed much to the fact that many metals are present in 'trace' amounts. I want to address myself today to the general principles underlying the evolution of metallobiochemistry and of its subdisciplines which did not exist a generation ago and give perspective to metals as toxic, environmental agents, a subject which has been of special interest to Prof. Houtman.The word 'trace' implies something that can be measured qualitatively but not quantitatively, and until about a generation ago that was true for many metals in biology. Therefore it was a nearly mystical subject explored of ten by individuals who had ideas about the problem but who performed few experiments to verify or reject them. This has changed. Measurement of very low concentrations of metals in biology has become quite routine. Technical problems have been eliminated to the point where the term 'trace' has become meaningless. That is fortunate, indeed, because to many 'trace' also meant 'unimportant' or 'insignificant' .Attention to the detection limits of analytical methods for metals has become almost irrelevant now. Yet, the fact that the II-B metals and those of the first transition series all occur in biological matter in similarly low concentrations led to the tacit inference that all of them would be found to perform similar functions. Such an emphasis on similarities of concentrations completely ignored the chemical properties of metals which are unique for each. Not too surprisingly, their biological roles have turned out to be as characteristic as their chemistries, enhanced by co-ordination of metals with 132 12 (1988) DELFT PROGRESS REPORT

show abstract

“…Another explanation suggests that a cuprous phenanthroline complex is the active form of the ligand (Fig. 10) (22,23). Indeed, several recent studies have inquired into the reaction of copper-phenanthroline, oxygen, a reducing agent, and DNA to degrade double-stranded DNA into acid-soluble fragments (24)(25)(26).…”

Section: Esr Investigations Of Tetradentate Cupric Complexesmentioning

confidence: 99%

ESR of Copper and Iron Complexes with Antitumor and Cytotoxic Properties

Antholine¹,

Kalyanaraman²,

Petering³

1985

Environmental Health Perspectives

View full text Add to dashboard Cite

The relatively few iron and copper metal complexes which have been examined in cells and tissues for their redox properties, radical generation properties, and antitumor activity are discussed for studies which utilized electron spin resonance spectroscopy (ESR). A common property of a number of metal complexes, which include bleomycin, adriamycin, and thiosemicarbazones described in this review, is that they are readily reduced by thiol compounds and oxidized by oxygen or reduced species of oxygen to produce radicals. Structural features ofthese reactions are identified by ESR spectroscopy in model systems and-often in cells. Furthermore, ESR spectroscopy has been most useful to probe the environment of the complexes in cells and to measure the rate of reduction of their oxidized forms. As a result of these studies, it is anticipated that more attention will be given to the exploration of redox-active metal complexes as drugs.

show abstract

Inhibition of E. coli DNA polymerase I by 1,10-phenanthroline

Cited by 37 publications

References 7 publications

Secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.

Secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.

The Metallobiochemistry of Zinc Enzymes

ESR of Copper and Iron Complexes with Antitumor and Cytotoxic Properties

Contact Info

Product

Resources

About