Inhibition of Enriched Stem Cells in Vivo and in Vitro by the Hemoregulatory Peptide SK&amp;F108636

Veiby, O. Petter; LoCastro, Stephen M.; Bhatnagar, Pradip K.; Olsen, Wenche Marie

doi:10.1002/stem.140215

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…Similarly bone marrow (BM) stem cells were isolated from mouse adult BM using published techniques for lineage depletion with magnetic separation (Miltenyi Biotec) followed by separation using flow cytometry and Sca1 antibody. Lin − /Sca1 + cells were used for BM transplantation [ 8 , 22 ]. Fetal liver (FL) stem cells were used for comparison.…”

Section: Methodsmentioning

confidence: 99%

Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice

Shangaris

Loukogeorgakis

Blundell

et al. 2018

Stem Cells and Development

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Clinical success of in utero transplantation (IUT) using allogeneic hematopoietic stem cells (HSCs) has been limited to fetuses that lack an immune response to allogeneic cells due to severe immunological defects, and where transplanted genetically normal cells have a proliferative or survival advantage. Amniotic fluid (AF) is an autologous source of stem cells with hematopoietic potential that could be used to treat congenital blood disorders. We compared the ability of congenic and allogeneic mouse AF stem cells (AFSC) to engraft the hematopoietic system of time-mated C57BL/6J mice (E13.5). At 4 and 16 weeks of age, multilineage donor engraftment was higher in congenic versus allogeneic animals. In vitro mixed lymphocyte reaction confirmed an immune response in the allogeneic group with higher CD4 and CD8 cell counts and increased proliferation of stimulated lymphocytes. IUT with congenic cells resulted in 100% of donor animals having chimerism of around 8% and successful hematopoietic long-term engraftment in immune-competent mice when compared with IUT with allogeneic cells. AFSCs may be useful for autologous cell/gene therapy approaches in fetuses diagnosed with congenital hematopoietic disorders.

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Section: Methodsmentioning

confidence: 99%

Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice

Shangaris

Loukogeorgakis

Blundell

et al. 2018

Stem Cells and Development

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show abstract

Hematoregulators

Bhatnagar

Huffman

King³

et al. 2002

Pharmaceutical Biotechnology

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Effects of GSM-Modulated Radiofrequency Electromagnetic Fields on Mouse Bone Marrow Cells

et al. 2008

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We examined the effects of in vivo exposure to a GSM-modulated 900 MHz RF field on the ability of bone marrow cells to differentiate, colonize lymphatic organs, and rescue lethally X-irradiated mice from death. X-irradiated mice were injected with medium alone or containing bone marrow cells from either RF-field-exposed (SAR 2 W/kg, 2 h/day, 5 days/ week, 4 weeks) or sham-exposed or cage control donor mice. Whereas all mice injected with medium alone died, mice that received bone marrow cells survived. Three and 6 weeks after bone marrow cell transplantation, no differences in thymus cellularity and in the frequencies of differentiating cell subpopulations (identified by CD4/CD8 expression) were observed among the three transplanted groups. Mitogen-induced thymocyte proliferation yielded comparable levels in all transplanted groups. As to the spleen, no effects of the RF-field exposure on cell number, percentages of B and T (CD4 and CD8) cells, B- and T-cell proliferation, and IFN-gamma production were found in transplanted mice. In conclusion, our results show no effect of in vivo exposure to GSM-modulated RF fields on the ability of bone marrow precursor cells to home and colonize lymphoid organs and differentiate in phenotypically and functionally mature T and B lymphocytes.

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Inhibition of Enriched Stem Cells in Vivo and in Vitro by the Hemoregulatory Peptide SK&F108636

Cited by 3 publications

References 40 publications

Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice

Long-Term Hematopoietic Engraftment of Congenic Amniotic Fluid Stem Cells After in Utero Intraperitoneal Transplantation to Immune Competent Mice

Hematoregulators

Effects of GSM-Modulated Radiofrequency Electromagnetic Fields on Mouse Bone Marrow Cells

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