Inhibition of Enteropathogenic Escherichia Coli (EPEC) Adherence to HeLa Cells by Human Colostrum. Detection of Specific sIgA Related to EPEC Outer-Membrane Proteins

Câmara, Lilia Maria Carneiro; Carbonare, S B; Scaletsky, Isabel C. A.; Silva, M. Lourdes Monteiro da; Carneiro‐Sampaio, Magda

doi:10.1007/978-1-4615-1941-6_142

Cited by 30 publications

(43 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although inhibition of EPEC adherence by sIgA milk antibodies has been previously demonstrated (4,5,12), identification of the molecular target of protective immunity has remained elusive. The present study showed that EPEC strains express many surface proteins that are recognized by sIgA antibodies obtained from the milk from Mexican women.…”

Section: Discussionmentioning

confidence: 99%

Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk

et al. 2000

View full text Add to dashboard Cite

Enteropathogenic Escherichia coli (EPEC) produces a characteristic attaching and effacing (A/E) lesion in the small intestines of infected children. The immune response to EPEC infection remains poorly characterized. The molecular targets that elicit protective immunity against EPEC disease are unknown. In this study protein antigens from EPEC were identified using secretory immunoglobulin A (sIgA) antibodies isolated from milk from Mexican women by Western blot analysis. Purified sIgA antibodies, which inhibit the adherence of EPEC to cells, reacted to many EPEC proteins, the most prominent of which were intimin (a 94-kDa outer membrane protein) and two unknown proteins with apparent molecular masses of 80 and 70 kDa. A culture supernatant protein of 110 kDa also reacted strongly with the sIgA antibodies. The molecular size of this protein and its reactivity with specific anti-EspC antiserum suggest that it is EPEC-secreted protein C (EspC).

show abstract

Section: Discussionmentioning

confidence: 99%

Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk

et al. 2000

View full text Add to dashboard Cite

show abstract

“…As additional controls we used human plasma purified C3 and an intravenous immunoglobulin preparation (Sandoglobulin, Sandoz, Basel) adjusted to a concentration of 14.0 g/l and used as 10% of total volume as the standard serum. This immunoglobulin preparation was analysed by western blotting and presented antibodies recognizing EPEC antigens involved in the bacterial pathogenicity in similar pattern as the control serum pool [8]. Purified C3 was obtained from a fresh human plasma pool according to the technique described by Tack et al [19].…”

Section: Methodsmentioning

confidence: 99%

“…IgA antibodies can block bacterial adherence to human epithelial cells as well as viral attachment and penetration [4]. Colostral IgA strongly inhibits in vitro EPEC adherence to HEp-2 and HeLa cells [6][7][8], as well as the invasion of the same cell lineages by enteroinvasive Escherichia coli (EIEC) [9].…”

Section: Introductionmentioning

confidence: 99%

Colostral Mononuclear Phagocytes are Able to Kill Enteropathogenic Escherichia coli Opsonized with Colostral IgA

Honório-França¹,

Carvalho²,

Isaac³

et al. 1997

Scand J Immunol

123

View full text Add to dashboard Cite

Honorio-França AC, Carvalho MPSM, Isaac L, Trabulsi LR, Carneiro-Sampaio MMS. Colostral Mononuclear Phagocytes are Able to Kill Enteropathogenic Escherichia coli Opsonized with Colostral IgA. Scand J Immunol 1997;46:59-66 Enteropathogenic Escherichia coli (EPEC) is the main aetiological agent of acute diarrhoea among low socioeconomic level infants in developing countries. Breast-feeding provides infant protection against acute gastrointestinal and respiratory infections; however, little is known about the protective role of colostral phagocytes in the gut of newborn infants. In the present investigation we studied the ability of human colostral MN phagocytes to kill EPEC as well as the interactions between these cells and colostral and serum opsonins. The authors observed that the microbicidal activity of colostrum MN phagocytes was dependent on previous EPEC opsonization with colostral supernatant or blood serum. A defatted colostrum supernatant pool presented opsonic activity for EPEC killing at levels equivalent to those of normal serum. IgA-depleted colostrum supernatant showed significantly lower opsonic activity, whereas purified IgA from the same colostrum pool was a potent opsonin which induced EPEC killing at levels equivalent to those of untreated colostrum. Colostral MN phagocytes are able to release superoxide anion when incubated with both EPEC opsonized with untreated colostrum and purified IgA. Purified IgA was also able to restore opsonic activity of IgA-depleted colostrum. A colostrum pool without C3 and IgG induced EPEC killing by colostral MN phagocytes at rates equivalent to those of untreated colostrum supernatant. Addition of an IgM MoAb (My43) anti-human Fca receptor resulted in a significant inhibition of EPEC killing when bacteria were opsonized with purified IgA, suggesting an interaction between IgA and FcaR. With respect to serum opsonins, we observed that IgG plus complement component C3 were necessary to induce EPEC killing by the colostrum MN phagocytes. Colostral phagocyte killing of enteropathogenic bacteria may represent an additional mechanism of breast-feeding protein against intestinal infections during the first week of life.

show abstract

“…The protective effect of human milk is usually attributed to its high content of immunoglobulin, specially secretory immunoglobulin A (sIgA) (6,16). Some works showed that sIgA inhibit the adhesion of enteropathogenic Escherichia coli to HeLa cells and Hep-2 (5,6,9), and possess effect against ETEC *Corresponding Author. Mailing address: Lab.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to Caco-2 cells by human milk and its immunoglobulin and non-immunoglobulin fractions

Oliveira¹,

Bessler²,

Báo³

et al. 2007

Braz. J. Microbiol.

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) is the most common cause of diarrhea in children in developing countries and among travelers to ETEC endemic areas. ETEC diarrhea is caused by colonization of the small intestine mediated by colonization factor (CF) antigens, and subsequent elaboration of enterotoxins. Breast feeding has been related to protection against enteric infections. The protective effect of human milk can be ascribed to its immunoglobulin content, specially secretory immunoglobulin A (sIgA), and to nonimmunoglobulin components such as free oligosaccharides, glycoproteins and glycolipids. In this study we investigated the effect of whole human milk and its fractions immunoglobulin and non-immunoglobulin on the adherence of ETEC strains possessing different CFs to Caco-2 cells, as well as the ability of sIgA and free secretory component (fSC) to bind to bacterial superficial proteins. Pooled human milk from three donors were fractionated by gel filtration and analyzed by SDS-PAGE. Our results revealed that whole human milk and its proteins fractions, containing sIgA and fSC, inhibited adhesion ETEC strains harboring different colonization factors antigens. We also verified that sIgA and fSC, using immunoblotting and immunogold labeling assays, bound to some fimbrial proteins and other material present in bacterial surface. Our findings suggest that whole human milk and its fractions may contribute to protection against ETEC infections by blocking bacterial adhesion mediated by different colonization antigens.

show abstract

Inhibition of Enteropathogenic Escherichia Coli (EPEC) Adherence to HeLa Cells by Human Colostrum. Detection of Specific sIgA Related to EPEC Outer-Membrane Proteins

Cited by 30 publications

References 11 publications

Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk

Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk

Colostral Mononuclear Phagocytes are Able to Kill Enteropathogenic Escherichia coli Opsonized with Colostral IgA

Inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to Caco-2 cells by human milk and its immunoglobulin and non-immunoglobulin fractions

Contact Info

Product

Resources

About