Inhibition of Escherichia coli DNA polymerase I by rose bengal

Photodynamic insecticides: A review of studies on photosensitizing dyes as insect control agents, their practical application, hazards, and residues

“…Chromosome breakage by both halogenated and non-halogenated xanthenes (rose bengal and rhodamine B) was seen in varied cell systems (Au andHsu 1979, Lewis et al 1981), im· munomodulatory activities by rhodamine B were described by Wess and Archer (1982) and DNA damage by phloxine, and possibly erythrosine, was discussed by Haveland-Smith and Combes (1980). The DNA polymerase I of E. coli is potently and irreversibly inhibited by photoactivated rose bengal and to only a slightly lesser extent by erythrosine (Stern et al 1980). The possible significance of these findings was amplified by McCann and Ames (1976) who have concluded that many chemical carcinogens probably initiate cancer through damage to DNA and who have shown that almost all carcinogens (90%) are also mutagens.…”

Binding of captan to DNA polymerase I from Escherichia coli and the concomitant effect on 5' .fwdarw. 3' exonuclease activity

“…This is the first report of a compound which reacts with a specific site in the template-primer binding locus and thereby causes an alteration in the physically distinct 5'-*3' exonuclease activity. Although other studies have used sitespecific modification to elucidate the catalytic relationships between the polymerase and 3'-*5' exonuclease activity, the 5'-* ' exonuclease activity was not examined (Que et al, 1979;Stern et al, 1980;Harza et al, 1984).…”