The Antiviral Effects of Rose Bengal and Fluorescein

Roat, Melvin I.; Romanowski, Eric G.; Araullo-Cruz, T; Gordon, Y. Jerold

doi:10.1001/archopht.1987.01060100117039

Cited by 29 publications

(7 citation statements)

References 21 publications

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“…The photodynamic inactivation of vaccinia virus by rose bengal was attributed to alterations in viral proteins, as opposed to nucleic acids (17). Rose bengal's virucidal activity against herpes simplex virus was noted as a complication attending its use in a common diagnostic procedure for ocular disease (18 Hemolysis. After illumination of the virus sample, 1.4 ml of buffer and 0.1 ml of a 10% suspension of fresh washed human erythrocytes in PBS were added, and the sample was mixed.…”

Section: Rose Bengal Hypeilnsupporting

confidence: 41%

Photodynamic inactivation of infectivity of human immunodeficiency virus and other enveloped viruses using hypericin and rose bengal: inhibition of fusion and syncytia formation.

Lenard¹,

Rabson

Vanderoef³

1993

Proc. Natl. Acad. Sci. U.S.A.

228

160

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The mechanism of the antiviral activity of hypericin was characterized and compared with that of rose bengal. Both compounds inactivate enveloped (but not unenveloped) viruses upon illumination by visible light. Human immunodeficiency and vesicular stomatitis viruses were photodynamically inactivated by both dyes at nanomolar concentrations. Photodynamic inactivation of fusion (hemolysis) by vesicular stomatitis, influenza, and Sendai viruses was induced by both dyes under similar conditions (e.g., I50 = 20-50 nM for vesicular stomatitis virus), suggesting that loss of infectivity resulted from inactivation of fusion. Syncytium formation, between cells activated to express human immunodeficiency virus gpl20 on their surfaces and CD4+ cells, was inhibited by illumination in the presence of 1 ,uM hypericin. Hypericin and rose bengal thus exert similar virucidal effects. Both presumably act by the same mechanism-namely, the inactivation of the viral fusion function by singlet oxygen produced upon illumination. The implications of this photodynamic antiviral action for the potential therapeutic usefulness of both hypericin and rose bengal are discussed.

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Section: Rose Bengal Hypeilnsupporting

confidence: 41%

Photodynamic inactivation of infectivity of human immunodeficiency virus and other enveloped viruses using hypericin and rose bengal: inhibition of fusion and syncytia formation.

Lenard¹,

Rabson

Vanderoef³

1993

Proc. Natl. Acad. Sci. U.S.A.

228

160

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“…HSV-2 was rarely isolated (Vannini 1986). No study reported whether a topical anæsthetic (Goldschmidt 2006; Weinberg 1977) or stain (Roat 1987; Seitzman 2006) might have curtailed viral detection.…”

Section: Resultsmentioning

confidence: 99%

Antiviral treatment and other therapeutic interventions for herpes simplex virus epithelial keratitis

Wilhelmus

2015

Cochrane Database of Systematic Reviews

107

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Background-Eye disease due to herpes simplex virus (HSV) commonly presents as epithelial keratitis which, though usually self-limiting, may persist or progress without treatment.Objectives-To compare the relative effectiveness of antiviral agents, interferon, and corneal debridement in the treatment of HSV epithelial keratitis. Search methods-We searched CENTRAL (which contains the Cochrane Eyes and Vision

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“…HSV-2 was rarely isolated (Vannini 1986). None determined whether diagnostic eye drops curtailed viral recovery (Roat 1987; Seitzman 2006). …”

Section: Resultsmentioning

confidence: 99%

Antiviral treatment and other therapeutic interventions for herpes simplex virus epithelial keratitis

Wilhelmus

2010

Cochrane Database of Systematic Reviews

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The Antiviral Effects of Rose Bengal and Fluorescein

Cited by 29 publications

References 21 publications

Photodynamic inactivation of infectivity of human immunodeficiency virus and other enveloped viruses using hypericin and rose bengal: inhibition of fusion and syncytia formation.

Photodynamic inactivation of infectivity of human immunodeficiency virus and other enveloped viruses using hypericin and rose bengal: inhibition of fusion and syncytia formation.

Antiviral treatment and other therapeutic interventions for herpes simplex virus epithelial keratitis

Antiviral treatment and other therapeutic interventions for herpes simplex virus epithelial keratitis

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