Inhibition of Gastric H⁺,K⁺-ATPase and Acid Secretion by Ellagic Acid

Abstract

The effects of ellagic acid on gastric H+, K(+)-ATPase, acid secretion, and the occurrence of gastric ulcers were studied. Ellagic acid inhibited hog gastric H+, K(+)-ATPase activity with a 50% inhibition at 2.1 x 10(-6)M; kinetic studies showed that the inhibition of H+, K(+)-ATPase by ellagic acid is competitive with respect to ATP and is noncompetitive with respect to K+. The effect on gastric ulcers was investigated by using a stress ulcer model. Intraperitoneal administration of ellagic acid at above 5 mg/kg markedly reduced the occurrence of gastric lesion. Ellagic acid significantly reduced acid secretion at the same doses. These results suggest that ellagic acid has a marked inhibitory effect on acid secretion and the occurrence of stress-induced gastric lesions, and these effects may be attributed to the inhibition of H+, K(+)-ATPase activity.

Antiulcer and antimicrobial activity of Anogeissus latifolia

“…The high percentage of the gallic acid and ellagic acid in the extract justifies the potent antioxidant activity exhibited (Gyamfi and Aniya, 2002). Ellagic acid (above 5 mg/kg) has been reported to markedly reduce the occurrence of gastric lesion in stressinduced ulcer models (Murakami et al, 1991). Hence, ellagic acid was chosen for the standardization of the extract.…”

“…Antioxidant and antimicrobial activity have also been studied as the role of the ROS in ulcers is definite. High performance thin layer chromatography (HPTLC) finger printing of the total extract was also carried out using gallic acid and ellagic acid as markers in an attempt to characterize the constituents responsible for the activities and also to standardize the extract, since antiulcer activity is reported for ellagic acid for (Murakami et al, 1991) …”

Evaluation of the antifungal activity and mode of action of Lafoensia pacari A. St.-Hil., Lythraceae, stem-bark extracts, fractions and ellagic acid

“…Previous works have shown that Lafoensia pacari stem bark ethanol and methanol extracts possess anti-ulcer (Tamashiro-Filho, 1999), anti-inflammatory (Albuquerque, 1996a, Rogerio et al, 2008a), immunostimulating (Albuquerque, 1996b, free radicals scavenging (Solon et al, 2000), antiviral (Müller et al, 2007), anti-eosinophilic (Rogerio et al, 2008b), analgesic and anti-oedematous (Rogerio et al, 2006) and inhibition of interleukin (IL)-5 production (Rogerio et al, 2003) activities. According to Solon (1999), this plant is rich in ellagic acid, which presents gastric anti-secretory (Murakami et al, 1991) and antibacterial effects (Chung, 1998;Lima et al, 2006) and a recent clinical study carried out with Lafoensia pacari extract revealed positive effects on the symptomatology of Helicobacter pylori infected patients (Mota Menezes et al, 2006), but to the best of our knowledge, there was no published report on the plant's antifungal properties.…”

Anti-ulcer activity of ethanol extract of Terminalia pallida Brandis. in Swiss albino rats

“…Ellagic acid, a widely occurring polyphenol, possesses strong antioxidant properties (Festa et al, 2001). It has a marked inhibitory effect on acid secretion and the occurrence of stress-induced gastric lesions, and these effects may be attributed to the inhibition of H + -and K + -ATPase activity (Murakami et al, 1991).…”