Inhibition of growth of microcultured Hancornia speciosa shoots by 3β-hydroxylated gibberellins and one of their C-3 deoxy precursors

Ab, Pereira-Netto; Bh, McCown; Rp, Pharis

doi:10.1007/s00299-002-0536-y

Cited by 6 publications

(4 citation statements)

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“…For mangaba tree, due to the high leaf abscission (Figure 2) shown in function of the ethylene concentration (PEREIRA NETTO, 2001;MCCOWN;PHARIS, 2003), the type of flask sealing is an important factor to be considered in the establishing of future largescale propagation protocols.…”

Section: Second Subculturementioning

confidence: 99%

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Sealing and explant types on the mangaba micropropagation

et al. 2012

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In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L -1 IAA and 1 mg L -1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro cultivation. The best types of flask sealing for the establishment phase were the PVC film and Para-film ® and for the first subculture the Para-film ® seal. In the second subculture the PVC film and Para-film ® seals promoted the best growth. The median and basal nodal segments presented the best performance in the first subculture. No significant effect of explant type was observed in the second subculture. The ideal subculture interval in the establishment phase and the first and second subcultures is 50 days. Index terms:Hancornia speciosa, tissue culture, in vitro ethylene, nodal segments. RESUMONa micropropagação, especialmente para mangaba, variedade botânica da árvore do Nordeste do Brasil, aspectos limitantes, como acúmulo de etileno no recipiente de cultivo e perda de vigor em subculturas têm sido observados. Neste estudo, objetivou-se avaliar o conhecimento técnico e científico da propagação in vitro de mangabeira, variedade botânica do Nordeste do Brasil, e melhorar o protocolo de micropropagação, o melhor tipo de vedação frasco e explante em diferentes etapas. Para a fase de estabelecimento e para as subculturas primeiro e segundo, foi utlizado o meio MS com 3% de sacarose e agar 0,6%, suplementado com 1 mg L -1 de AIA e 1 mg L -1 de BAP. As avaliações foram realizadas aos 30, 50 e 65 dias de cultivo in vitro. Os melhores tipos de vedação para a fase de estabelecimento foram o filme PVC e Para-filme ® e, para o primeiro subcultivo, o Para-filme ® . No segundo subcultivo, o filme PVC e Para-filme ® promoveram o melhor crescimento in vitro. Os segmentos nodais médio e basal apresentaram melhor desempenho no primeiro subcultivo. Nenhum efeito significativo do tipo de explante foi observado no segundo subcultivo. O intervalo de tempo ideal para as fases de estabelecimento, primeiro e segundo subcultivos é de 50 dias. Termos para indexação:Hancornia speciosa, cultura de tecidos, etileno in vitro, segment nodal.

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Section: Second Subculturementioning

confidence: 99%

“…Studies on the ethylene metabolism and in vitro leaf abscission control by inhibiting the production of this growth regulator in mangaba tree have been conducted (PEREIRA NETTO, 2001;MCCOWN;PHARIS, 2003).…”

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confidence: 99%

Sealing and explant types on the mangaba micropropagation

et al. 2012

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“…• The methodology used in this study was able to adequately measure photosynthetic responses in H. speciosa seedlings grown in vitro, indicating a relative functionality of the photosynthetic apparatus of these species under the conditions of this study and exhibiting substantial rates of CO 2 assimilation under high levels of irradiance. (Pereira-Netto e McCown, 1999;Pereira-Netto et al, 2003;Costa et al, 2014;Dantas et al, 2021) Rooting/ Acclimatization…”

Section: Micropropagationmentioning

confidence: 99%

Advances and perspectives in the propagation of mangabeira (Hancornia speciosa Gomes) - review on a tropical fruit tree with socioeconomic importance

Nascimento,

Santos,

Muniz

et al. 2024

CERNE

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Background: Hancornia speciosa is a species known for its medicinal and nutritional properties. Despite being a plant native to several regions of Brazil, some aspects of its production system are not yet well defined. Large-scale asexual propagation stands out, making it difficult to establish commercial orchards with materials with superior characteristics. In this context, this article aimed to investigate the scientific production related to the propagation and conservation of the mangabeira, using a mixed methodology of bibliometric analysis and literature review. The Scopus and Web of Science databases were consulted and the VOSviewer software was used to map the cooperation network between authors, institutions, and keywords. Results:The study included 72 articles and found a significant increase in scientific production on mangabeira propagation and conservation since 2009. Most studies focused on seed technology, seedling production, in vitro propagation and conservation, and cryopreservation. Additionally, it was observed that mangabeira seeds do not tolerate a reduction in moisture content, and the combination of materials for the composition of the substrate, such as coconut fiber, manure, and sand, can provide an adequate basis for developing seedlings. It was also observed that MS culture medium, complete or with half saline concentration, or WPM medium can be used for in vitro germination and initial seedling growth. Conclusion:Through this review, we concluded that the mangabeira presents substantial potential for the development of innovative propagation techniques, with a special focus on methods that facilitate the clonal propagation of the species, such as grafting, rooting cuttings, and micropropagation. It is expected that future research will expand knowledge about the in vitro and ex vitro propagation of this species, contributing to the expansion of commercial plantations with a focus on adding to the processing of its fruits.

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“…A utilização da cultura de tecidos para a propagação dessa espécie vem sendo estudada, porém poucos resultados satisfatórios foram relatados, devido às difi culdades intrínsecas da planta. Estudos visando a aumentar o número de brotações in vitro foram realizados por Ribeiro et al (1998) e Pereira Netto et al (2003, porém ainda há necessidade da otimização do protocolo para essa espécie.…”

Section: Evaluation Of Exogenous Application Of Polyamines On Callus unclassified

Avaliação da aplicação exógena de poliaminas no crescimento de calos de mangabeira (Hancornia speciosa Gomes)

Fráguas¹,

Villa²,

Lima

2009

Rev. Bras. Frutic.

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RESUMO -Este trabalho teve como objetivo estudar o efeito das poliaminas espermidina e espermina no crescimento de calos Hancornia speciosa Gomes. Calos com 0,5 cm de diâmetro foram inoculados em meio Murashige & Skoog (1962) ) e espermina (868,53 μg g mf -1 ) aos 40 dias de cultivo, quando se aplicou a própria poliamina. Conclui-se que a aplicação exógena de poliaminas em Hancornia speciosa não proporciona aumento no crescimento de calos. A oxidação promovida por longos períodos de cultivo in vitro induz aumento nos níveis de putrescina. Termos para indexação: cultura de tecidos, mangabeira, putrescina, espermina, espermidina. There was not polyamine infl uence in the calluses growth. It was observed in the treated calluses with spermidine larger cellular putrescine concentration (582.37 μg g mf-1) at 60 days, larger spermidine concentration (502.54 μg g mf-1) and spermine (868.53 μg g mf-1) at 40 days of cultivation, when the own polyamine was applied. It was concluded that the exogenous polyamine application in Hancornia speciosa does not provide an increase in the calluses growth. The oxidation promoted by long periods of in vitro cultivation induces an increase in the putrescine levels. Index terms: tissue culture, mangaba tree, putrescine, spermine, spermidine. EVALUATION OF EXOGENOUS APPLICATION OF POLYAMINES ON CALLUS GROWTH OF MANGABA TREE (Hancornia speciosaGomesA mangabeira (Hancornia speciosa Gomes) é uma planta originária do Brasil e apresenta ampla dispersão geográfi ca, ocorrendo em diversos ecossistemas, como Amazônico, Mata Atlântica e Cerrado (Donadio et al., 2002). Apesar do grande potencial apresentado pela mangabeira como planta frutífera e até mesmo como produtora de borracha, e face à inexistência de plantios racionais e tecnifi cados, o extrativismo apresenta-se, atualmente, como única forma de exploração, constituindo-se, assim, numa grande barreira ao aproveitamento de todas as suas potencialidades.A forma convencional de propagação desta planta é através de sementes, uma vez que a propagação por estaquia não fornece bons resultados (Espíndola et al., 1993; Vieira Neto, 1994). A utilização da cultura de tecidos para a propagação dessa espécie vem sendo estudada, porém poucos resultados satisfatórios foram relatados, devido às difi culdades intrínsecas da planta. Estudos visando

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Inhibition of growth of microcultured Hancornia speciosa shoots by 3β-hydroxylated gibberellins and one of their C-3 deoxy precursors

Cited by 6 publications

References 35 publications

Sealing and explant types on the mangaba micropropagation

Sealing and explant types on the mangaba micropropagation

Advances and perspectives in the propagation of mangabeira (Hancornia speciosa Gomes) - review on a tropical fruit tree with socioeconomic importance

Avaliação da aplicação exógena de poliaminas no crescimento de calos de mangabeira (Hancornia speciosa Gomes)

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