Inhibition of histone-deacetylase activity by short-chain fatty acids and some polyphenol metabolites formed in the colon

Waldecker, Markus; Kautenburger, Tanja; Daumann, Heike; Busch, Cordula; Schrenk, Dieter

doi:10.1016/j.jnutbio.2007.08.002

Cited by 504 publications

(389 citation statements)

References 23 publications

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“…In addition to diet, pollution, and infections, our study highlights the intestinal microbiota as another factor affecting epigenetic gene modification. Short-chain fatty acids such as butyrate, which are produced by intestinal microbiota, are known to act as a histone deacetylase inhibitor (30,31), also suggesting epigenetic regulation by commensal bacteria, although the effect on transcription is opposite to that observed in our study.…”

Section: Discussioncontrasting

confidence: 85%

Epigenetic Control of the Host Gene by Commensal Bacteria in Large Intestinal Epithelial Cells

Takahashi

Sugi

Nakano

et al. 2011

Journal of Biological Chemistry

151

123

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Background:Intestinal epithelial cells (IECs) express low levels of TLR4 and are hyporesponsive to commensal bacteria. Results: TLR4 gene is methylated in IECs, and this process is dependent on commensal bacteria in the large intestine. Conclusion: Commensal bacteria control epigenetic modification of the host gene. Significance: This study shows a novel mechanism underlying the maintenance of intestinal symbiosis.

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Section: Discussioncontrasting

confidence: 85%

Epigenetic Control of the Host Gene by Commensal Bacteria in Large Intestinal Epithelial Cells

Takahashi

Sugi

Nakano

et al. 2011

Journal of Biological Chemistry

151

123

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“…Butyrate plays a crucial role in maintaining colonic epithelial integrity by regulating epithelial proliferation and differentiation (Zhang & Ohta 1993;Siavoshian et al 2000). Butyrate has been reported to induce cell-cycle inhibitors and promoting apoptosis of transformed colonocytes through the mechanisms associated with inhibition of histone deacetylase activity and induction of p21 WAF1/Cip1 and cyclin D3 (Boren et al 2003;Comalada et al 2006;Waldecker et al 2008). Butyrate significantly reduces the cellular protein kinase C activities which play a role in the regulation of differentiation in colon cancer cell lines (Duan et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Hydrolysed inulin alleviates the azoxymethane-induced preneoplastic aberrant crypt foci by altering selected intestinal microbiota in Sprague–Dawley rats

Pattananandecha

Sirilun

Duangjitcharoen

et al. 2016

Pharmaceutical Biology

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Context Inulin, a non-digestible carbohydrate isolated from Helianthus tuberosus L. (Asteraceae), has been shown to alter the gut beneficial bacteria including Lactobacillus spp. and Bifidobacteria. Inulin also influences the activities of intestinal microbiota that could prevent the colon cancer development. Objective This study determines the effect of hydrolysed inulin with different degrees of polymerisation on alteration of intestinal microbiota and their activities on azoxymethane (AOM)-induced preneoplastic aberrant crypt foci (ACF) in rats. Materials and methods Seventy-two male Sprague-Dawley rats were randomly divided into six groups (three control and three AOM-treated groups) and the animal were fed with either a normal diet or diet containing 10% of long-chain inulin (InuL) or short-chain inulin (InuS), respectively, for 17 weeks. Colon cancer was induced in rats by injecting AOM subcutaneously at the 8th and 9th week of the study period. At the end of the experiment, cecal contents of rats were examined for selected microbiota, organic acids, putrefactive compounds and microbial enzymes. ACF formation was microscopically examined. Results The inulin diets significantly increased the weight and decreased the pH of the caecal content. The rats fed with InuL-supplemented diet showed approximately 2.9-and 6.8-fold increases in the biomass of Lactobacillus spp. and Bifidobacteria, respectively. Naive and AOMtreated rats fed with inulin-supplemented diet showed $1.3-and $2.2-fold decreases in the biomass of Escherichia coli and Salmonella enterica serovar Typhi, respectively. Inulins significantly decreased the colonic concentration of phenol, p-cresol and indole. Reduction in the activity of microbial enzymes such as b-glucuronidase, azoreductase and nitroreductase were observed in inulin-treated animals. Reduction in the ACF formation has been observed in inulin-treated groups. Discussion and conclusion The present study demonstrates that dietary administration of inulin reduces the formation of preneoplastic lesions in the colon, possibly by altering the microecology and microbial activities on carcinogenesis.ARTICLE HISTORY

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“…The cytotoxic effects of sodium acetate on human cells have been extensively studied. For example, acetate inhibits the histone deacetylase (HDAC) activity via tissue-or cellspecific mechanisms (Waldecker et al, 2008;Soliman and Rosenberger, 2011). Rodent neutrophils exposed to acetate undergo global inhibition of HDAC activity (Vinolo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line

Yuan

Zhang

Jin³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The objective of this study was to investigate the effect of sodium acetate on the viability of the human gastric adenocarcinoma (AGS) epithelial cell line. AGS cells were exposed to a range of concentrations of sodium acetate for different periods of time, and the sodium acetate-induced cytotoxic effects, including cell viability, DNA fragmentation, apoptotic gene expression, and caspase activity, were assessed. The changes in these phenotypes were quantified by performing a lactate dehydrogenase cell viability assay, annexin V staining, terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL), and several caspase activity assays. In vitro studies demonstrated that the cytotoxicity of sodium acetate on the AGS cell line were dose-and time-dependent manners. No differences 2 Y. Xia et al. Genetics and Molecular Research 15 (4): gmr.15048375 were found between the negative control and sodium acetate-treated cells stained with annexin V and subjected to the TUNEL assay. However, caspase-3 activity was increased in AGS cells exposed to sodium acetate. Overall, it was concluded that sodium acetate exerted an apoptotic effect in AGS cells via a caspase-dependent apoptotic pathway.

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Inhibition of histone-deacetylase activity by short-chain fatty acids and some polyphenol metabolites formed in the colon

Cited by 504 publications

References 23 publications

Epigenetic Control of the Host Gene by Commensal Bacteria in Large Intestinal Epithelial Cells

Epigenetic Control of the Host Gene by Commensal Bacteria in Large Intestinal Epithelial Cells

Hydrolysed inulin alleviates the azoxymethane-induced preneoplastic aberrant crypt foci by altering selected intestinal microbiota in Sprague–Dawley rats

Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line

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