Inhibition of hsa_circ_0001313 (circCCDC66) induction enhances the radio-sensitivity of colon cancer cells via tumor suppressor miR-338-3p

Wang, Li; Peng, Xiuda; Lu, Xianzhou; Wei, Qinglan; Chen, Ming-Dao; Liu, Longfei

doi:10.1016/j.prp.2018.12.032

Cited by 77 publications

(51 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TICs are depicted as the main factor that make tumors difficult to eradicate owing to the therapies only targeting the majority of tumor cells not TICs (O'Connor et al, 2014). Additionally, abundant investigations have elucidated the critic regulatory role of circular RNAs in breast cancer , colon cancer (Wang et al, 2019b) and NSCLC .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Withdrawn: Circ‐SOX4 promotes non‐small cell lung cancer progression by activating the Wnt/β‐catenin pathway

Wang

Cheng

et al. 2020

Molecular Oncology

View full text Add to dashboard Cite

Abbreviation Non-small-cell lung carcinoma (NSCLC) Tumor-initiating cells (TICs) circular RNAs (circRNAs) lung adenocarcinoma (LUAD) lung squamous cell carcinoma (LUSC) Quantitative real-time PCR (qRT-PCR) Cell Counting Kit-8 (CCK-8) Chromatin immunoprecipitation (ChIP) RNA Immunoprecipitation (RIP) Immunohistochemistry (IHC) genomic DNA (gDNA) Running heading: Circ-SOX4 regulates c-MYC. AbstractNon-small-cell lung carcinoma (NSCLC) accounts for the majority of lung cancer deaths, and thus there is a clinical need to understand the molecular mechanisms underlying this malignancy. Tumor-initiating cells (TICs) have been accepted to be closely related to tumor reoccurrence after surgery, and circular RNAs (circRNAs) play a crucial function in the tumorigenesis and development of human cancers.Here, we examined the role of the circular RNA hsa_circ_0131457 (circ-SOX4) in lung TICs. We report that circ-SOX4 is upregulated and exists as a covalently closed loop structure in CD133 + cancer cells. Subsequent functional assays showed that circ-SOX4 downregulation suppresses lung TIC proliferation, self-renewal, migration and invasion. Circ-SOX4 upregulation facilitates in vitro development of CD133cancer cells, and circ-SOX4 deficiency represses in vivo malignancy of CD133 + cells. Additionally, circ-SOX4 was shown to interact with c-MYC by activating the Wnt/β-catenin pathway in NSCLC. In summary, circ-SOX4 expedites NSCLC progression by activating the Wnt/β-catenin pathway, and this finding may facilitate the identification of effective therapeutic targets for NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

“…For example, circular RNA hsa_circ_0052112 promotes breast cancer cell metastasis by sponging miR-125a-5p . Circular RNA hsa_circ_0001313 is associated with the radio-sensitivity of colon cancer cells (Wang et al, 2019b). Circular RNA hsa_circ_0000144 regulates bladder cancer progression by targeting miR-217/RUNX2 axis (Huang et al, 2018).…”

mentioning

confidence: 99%

Withdrawn: Circ‐SOX4 promotes non‐small cell lung cancer progression by activating the Wnt/β‐catenin pathway

Wang

Cheng

et al. 2020

Molecular Oncology

View full text Add to dashboard Cite

show abstract

“…Shuai et al stated that circ_0000285 regulated the radiosensitivity of nasopharyngeal carcinoma and served as a prognostic indicator [30]. Wang et al addressed that circ_0001313 was remarkably upregulated in colon cancer cells treated with radiation, and circ_0001313 silence enhanced radiosensitivity to block cell viability and colony formation [13]. Su et al utilized circRNA microarray to identify differently expressed circRNAs in radioresistant esophageal cancer cells and parental cells, and circ_0086720 was highly expressed in radioresistant esophageal cancer cells [11], hinting that circ_0086720 might play a vital role in radioresistance of esophageal cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, they have higher stability compared to linear RNAs and are more suitable as biomarkers in different human diseases [10]. Recently, circRNAs were reported to be dysregulated in radioresistant and radiosensitive tumor tissues and play functions in radioresistance in various cancers [11,12], such as circ_0001313 in colon cancer [13], circ_100367 in esophageal squamous cell carcinoma [14], and circ_000543 in nasopharyngeal carcinoma [15]. In NSCLC, numerous circRNAs were documented to promote or suppress cancer progression [16,17].…”

mentioning

confidence: 99%

Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis

Jin

Sheng

et al. 2021

neo

View full text Add to dashboard Cite

Radioresistance is an important cause of cancer treatment failure. Circular RNAs (circRNAs) play crucial roles in cancer development, including the radioresistance. This research aimed to determine the function and related mechanism of circ_0086720 in the radioresistance of non-small cell lung cancer (NSCLC). The expression of circ_0086720, miR-375, and Spindlin 1 (SPIN1) was measured using a quantitative real-time polymerase chain reaction (qRT-PCR). Cell survival fraction was analyzed using colony formation assay, and cell apoptosis was monitored b y flow cytometry assay. The activities of caspase3 and caspase9 were assessed using the corresponding commercial kits. The ed by western blot. Bioinformatics analysis was performed to predict the targets of circ_0086720 and miR-375. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, and RNA pull-down assay were conducted to validate the interaction between miR-375 and circ_0086720 or SPIN1. The animal model was constructed to ascertain the role of circ_0086720 in vivo. The expression of circ_0086720 and SPIN1 was increased in the radioresistant NSCLC tissues, while miR-375 expression was decreased. The circ_0086720 knockdown sensitized NSCLC cells to the radiation to further inhibit cell survival and induce cell apoptosis. Circ_0086720 targeted miR-375 and suppressed miR-375 expression, and miR-375 bound to SPIN1 to impair SPIN1 expression. miR-375 deficiency or SPIN1 overexpression could attenuate circ_0086720 knockdown-mediated radiosensitivity. The circ_0086720 knockdown also enhanced radiosensitivity to further block tumor growth in vivo. To conclude, circ_0086720 downregulation enhanced the sensitivity of NSCLC to radiation by regulating the miR-375/SPIN1 axis, contributing to the improvement of the radiotherapies in NSCLC.

show abstract

“…CircCCDC66 is increased in CRC tissues and is much higher in radioresistant tissues than in radiosensitive ones and is associated with decreased levels of miR-338-3p. Since circCCDC66 sponges miR-338-3p, silencing circCCDC66 inhibits cell viability and enhances CRC cell radiosensitization via caspase-3 [108].…”

Section: Colorectal Cancermentioning

confidence: 99%

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

et al. 2020

View full text Add to dashboard Cite

Circular RNAs (circRNAs), one type of non-coding RNA, were initially misinterpreted as nonfunctional products of pre-mRNA mis-splicing. Currently, circRNAs have been proven to manipulate the functions of diverse molecules, including non-coding RNAs, mRNAs, DNAs and proteins, to regulate cell activities in physiology and pathology. Accumulating evidence indicates that circRNAs play critical roles in tumor genesis, development, and sensitivity to radiation and chemotherapy. Radiotherapy and chemotherapy are two primary types of intervention for most cancers, but their therapeutic efficacies are usually retarded by intrinsic and acquired resistance. Thus, it is urgent to develop new strategies to improve therapeutic responses. To achieve this, clarification of the underlying mechanisms affecting therapeutic responses in cancer is needed. This review summarizes recent progress and mechanisms of circRNAs in cancer resistance to radiation and chemotherapy, and it discusses the limitations of available knowledge and potential future directions.

show abstract

Inhibition of hsa_circ_0001313 (circCCDC66) induction enhances the radio-sensitivity of colon cancer cells via tumor suppressor miR-338-3p

Cited by 77 publications

References 22 publications

Withdrawn: Circ‐SOX4 promotes non‐small cell lung cancer progression by activating the Wnt/β‐catenin pathway

Withdrawn: Circ‐SOX4 promotes non‐small cell lung cancer progression by activating the Wnt/β‐catenin pathway

Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

Contact Info

Product

Resources

About