Inhibition of human basophil and rat mast cell activation by avene spring water

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The biological activity of Avène water from two different springs (‘Sainte Odile’ and ‘Val d’Orb’) was studied in vitro on rat peritoneal mast cell activation. A dilution-dependent inhibition of both histamine and prostaglandin D₂ antigen-induced release was observed when cells were preincubated with both Avène spring waters. They also inhibited histamine release triggered by substance P. The ability of Avène water to inhibit mast cell activation in vitro may be related with its antiallergic and anti-inflammatory properties and its use in hydrotherapy.

“…In contrast to peptidergic activation, antigenic stimulation of rat mast cells resulted in the release of the newly formed mediator PGD 2 …”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of Avène Spring Water on the Activation of Rat Mast Cell by Substance P or Antigen

Joly

Charvéron

Ariès

et al. 1998

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“…results]. ASW was also shown to inhibit the in vitro human basophil allergen-induced activa tion [1], This inactivation, which involved the degranulation process, suggested an effect of the ASW at the plasma membrane level.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Avène Spring Water on the Dynamics of Lipids in the Membranes of Cultured Fibroblasts

Cézanne

Gaboriau²,

Charvéron³

et al. 1993

In this preliminary report, dealing with the biological properties of AvÈne spring water, we investigated its effects on membrane properties of cultured human skin fibroblasts used as a model cell system. It is shown that incubation of these cells in the presence of AvÈne spring water brings about an increase in the fluidity of plasma membrane. This effect was evidenced by a decrease in the fluorescence anisotropy of the lipophilic probe diphenylhexatriene and by a 3-fold increase in the lateral diffusion coefficient of the lipophilic probe 5-(N-hexade-canoyl)-aminofluorescein, as measured through fluorescence recovery after photobleaching experiments. This effect reached a maximum for incubation times longer than 30 min and for a dilution of AvÈne water in laboratory water to about 20–25%. As discussed, changes in the hydrodynamic properties of cell membranes induced by AvÈne water provide a plausible explanation for some of the biological events this spring water is known to trigger.

“…The two other responses in vitro which described inhibition of basophil degranulation [21] and inhibition of rat mast cell histamine release [22] in the presence of ASW agree with clinical prescriptions especially recommended for ASW, i.e. AD.…”

Section: Discussionmentioning

confidence: 65%

Immunomodulation Induced by Avène Spring Water on Th1- and Th2-Dependent Cytokine Production in Healthy Subjects and Atopic Dermatitis Patients

Portalès

Ariès

Licu

et al. 2001

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Avène spring water (ASW) is commonly used in France for treating atopic dermatitis and psoriasis. Previous works demonstrated modulation of cell membrane fluidity by ASW. The aims of the present study were (a) to investigate a possible in vitro effect of ASW on Th1- and Th2-dependent cytokine production using peripheral blood mononuclear cells from healthy individuals and (b) to investigate both the in vitro effect of ASW on AD patients’ cells and the in vivo cellular and clinical modifications induced by a 3-week Avène Medical Spa water cure (AMSWC). The effect of ASW was tested on lymphocyte cultures, which were stimulated in vitro by various mitogens and a superantigen of staphylococcal origin. The lymphocyte proliferation and the production of the cytokines IL-2, IL-4 and IFN-γ were tested. The results showed that ASW-containing medium enhanced the lymphoproliferative response to some mitogens. IL-2 and IFN-γ production were also increased in stimulated culture supernatants. Conversely, ASW-containing medium induced a decrease in IL-4 production by normal peripheral blood lymphocytes. Furthermore, AMSWC was able to amend the clinical features as well as the immunological Th2 profile of atopic dermatitis.