Inhibition of Human MCF-7 Breast Cancer Cells and HT-29 Colon Cancer Cells by Rice-Produced Recombinant Human Insulin-Like Growth Binding Protein-3 (rhIGFBP-3)

Cheung, Stanley C. K.; Long, Xiaohang; Liu, Lizhong; Liu, Qiaoquan; Lan, Linda; Tong, Peter C.Y.; Sun, Samuel S. M.

doi:10.1371/journal.pone.0077516

Cited by 6 publications

(3 citation statements)

References 57 publications

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“…其 N 端都含有一段信号肽序列, 长度随物种和贮藏蛋白种类的不同而异, 它们在贮藏蛋白及其 mRNA 分子的定位及运输中起重要作用 [4,23] 。水稻醇溶蛋白点与热点 [27][28][29] 。尤其是近年来, 利用植物这一廉价生产系统作为生物反应器, 来大量生产重组的编码具重要功能的异源蛋白如医用活性多肽或疫苗、抗体等, 是当前植物基因工程发展的一个重要领域之一 [30][31][32][33][34] 。但是, 在植物生物反应器研究中存在一个较为突出的问题, 即目标蛋白的表达量极低, 某些蛋白还不能稳定积累下来 [30][31]…”

Section: 植物种子贮藏蛋白基因在翻译成前体蛋白时unclassified

Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of Glutelin GluA-2 in Endosperm of Transgenic Rice

王红梅¹,

张昌泉²,

李钱峰³

et al. 2015

Acta Agronomica Sinica

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It is one of the key important techniques to enhance the expression of foreign proteins in target tissue/organ of transgenic plants. Glutelin is the major component of storage proteins in rice seeds, and its expression was tightly temporal and tissuespecific, which is controlled by several mechanisms. To further reveal the function of the Glutelin signal peptide on expression of target gene, in present study, we isolated the promoter and signal peptide-coding sequences of the glutelin GluA-2 gene, and fused them transcriptionally to the GUS coding sequences. Beside, the construct without the GluA-2 signal peptide-coding sequences was also generated as a control. Both constructs with the GUS chimeric genes, named as p13GSG and p13GG, were introduced into the same rice variety by Agrobacterium-mediated transformation. More than twenty independent transgenic lines were generated for each construct, and the integration of the GUS chimeric gene was confirmed by PCR technique. The results from Northern blot analysis showed that, after fusing the GluA-2 signal peptide coding sequences between the GluA-2 promoter and the GUS coding sequence, the transcription of GUS chimeric gene could be dramatically increased. Then, Western blot was carried out by using the GUS-specific antibody, and the results obviously revealed that the accumulation of foreign proteins was significantly enhanced in the endosperm of transgenic rice with the signal peptide. However, there was no or very low GUS activity in theendosperm of transgenic rice plants with the signal peptide. These results were very useful to improve the grain quality of rice via genetic engineering, especially produce foreign proteins in the seeds of rice as bioreactor.

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Section: 植物种子贮藏蛋白基因在翻译成前体蛋白时unclassified

Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of Glutelin GluA-2 in Endosperm of Transgenic Rice

王红梅¹,

张昌泉²,

李钱峰³

et al. 2015

Acta Agronomica Sinica

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“…Downregulation of IGFBP3 upregulated tumor growth and suppressed apoptotic activity (25). IGFBP3 has potential as a drug, and the 16-kDa IGFBP3 fragment could potentiate apoptosis (26)(27)(28). IGFBP3 overexpression enhanced chemotherapy-induced growth inhibition due to inhibiting NF-kB (29).…”

Section: Introductionmentioning

confidence: 99%

Insulin-like Growth Factor-1, Insulin-like Growth Factor Binding Protein-3 and the Incidence of Malignant Neoplasms in a Nested Case–Control Study

Adachi

Nojima

Mori

et al. 2020

Cancer Prevention Research

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Insulin-like growth factor (IGF)-1 is a potent mitogen, but IGF binding protein (IGFBP)-3 inhibits IGF1. To elucidate the relationship between both IGF1 and IGFBP and the risk of tumorigenesis, the association between IGF1 and IGFBP3 serum levels and of malignant tumor incidence was investigated in a prospective case-control study nested in the Japan Collaborative Cohort Study. A baseline survey was started in [1988][1989][1990] 110,585 subjects were enrolled, and 35% of participants donated blood samples. Those who had been diagnosed with malignant tumors by 1997 were considered cases. The analysis involved 1,349 cases and 4,012 controls. Conditional logistic regression was used to estimate ORs for cancer incidence associated with IGF-related molecules. After controlling for alcohol intake, body mass index (BMI), and smoking, participants with high total-IGFBP3 and free-IGFBP3, which is estimated by the molar difference of (IGFBP3 À IGF1), had a risk of future neoplasms (P trend ¼ 0.014 and 0.009, respectively), but those with IGF1 did not. People in the second to fifth quintiles had a lower risk than those in the first quintile (ORs 0.676-0.736 and 0.657-0.870, respectively). Limiting subjects to those followed for 3 years weakened the negative associations of total-and free-IGFBP3, whereas a positive relationship of free-IGF1, which was estimated by the molar ratio of IGF1/IGFBP3, was seen (P trend ¼ 0.004, 0.002, and 0.013, respectively). After controlling for alcohol intake, smoking, BMI, and diabetes mellitus, the results were confirmed. These findings suggest that serum IGF1 and IGFBP3 are related to future risk of malignant neoplasms.

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“…63,67,68 However, significantly reduced IGFBP-3 concentrations have been shown to have an inverse relationship with the risk of cancers 63,69,70 as well as tumor migration, 71,72 leading to tumor growth and suppressed apoptosis. 73,74 In addition, reduced IGFBP-3 concentrations could indicate a stronger immune system, which plays an important role in the suppression of tumors.…”

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confidence: 99%

Serum Lipids, Insulin-Like Growth Factor Binding Protein-3 and Treatment Outcomes in Women with and without Cervical Lesions in South Western Uganda: A Cohort Study

Ssedyabane,

Randall,

Tusubira

et al. 2023

PLMI

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There is a documented association between cervical cancer and metabolic syndrome. In this study, we determined the association between lipids, insulin-like growth factor binding protein-3 (IGFBP-3) and treatment outcomes for cervical lesions at Mbarara Regional Referral Hospital (MRRH) in south western Uganda. Patients and Methods: In this prospective cohort study, we recruited 94 cases and 94 controls at the cervical cancer clinic of MRRH, and followed them for 12 months. Cases were confirmed with cytology and/or histology. With consent, we collected demographic data and measured lipids and IGFBP-3 both at baseline and after 12 months. The lipid profile was measured using a Cobas 6000 Chemistry Analyzer, whereas IGFBP-3 was measured using a MAGLUMI fully automated chemiluminescence immunoassay analyzer. Abnormal values for lipids were defined using WHO recommended cut-offs. IGFBP-3 concentrations were divided into two categories of low concentration (<3.29 µg/mL) and raised concentration (≥3.29 µg/mL). Statistical analyses were conducted in STATA version 17 using logistic regression analysis. A p-value of <0.05 was taken to be statistically significant. Results:The average (mean ± SD) age of our participants was 38.7±8.2 years for controls and 34.5±7.8 years for cases. The average serum IGFBP-3 concentration was 3.769±1.098 µg/mL among cases with cleared lesions and 3.505±0.979 µg/mL among cases whose lesions persisted. A serum IGFBP-3 concentration of less than 3.291 µg/mL was likely to be associated with clearance of cervical lesions (AOR 1.65, p=0.67), although this was not statistically significant. A serum triglyceride concentration of 35-135 mg/dL was also likely to be associated with clearance of cervical lesions (AOR 2.41, p=0.46), although this was also not statistically significant. Conclusion:Although not statistically significant, reduced serum concentrations of IGFBP-3 and triglyceride may be associated with clearance of cervical lesions. Lipid management may be of benefit in the treatment of cervical lesions.

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Inhibition of Human MCF-7 Breast Cancer Cells and HT-29 Colon Cancer Cells by Rice-Produced Recombinant Human Insulin-Like Growth Binding Protein-3 (rhIGFBP-3)

Cited by 6 publications

References 57 publications

Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of Glutelin GluA-2 in Endosperm of Transgenic Rice

Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of Glutelin GluA-2 in Endosperm of Transgenic Rice

Insulin-like Growth Factor-1, Insulin-like Growth Factor Binding Protein-3 and the Incidence of Malignant Neoplasms in a Nested Case–Control Study

Serum Lipids, Insulin-Like Growth Factor Binding Protein-3 and Treatment Outcomes in Women with and without Cervical Lesions in South Western Uganda: A Cohort Study

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