Inhibition of Hyaluronidase Activity by<i>Vitis rotundifolia</i>. (Muscadine) Berry Seeds and Skins

Bralley, Eve E; Greenspan, Phillip; Hargrove, James L.; Hartle, Diane K.

doi:10.1080/13880200701545018

Cited by 24 publications

(13 citation statements)

References 45 publications

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“…The hyaluronidase‐inhibiting activity assay was performed following the methodology reported by Bralley et al, with adaptations: 147 μL of acetate buffer (0.2 mol L −1 sodium acetic acid at pH 6 with 0.15 mol L −1 NaCl) was added to 15 μL of diluted sample or standard. 120 μL of 0.5 mg mL −1 sodium hyaluronate were added and mixed for 30 s. Then 1 mg mL −1 hyaluronidase was added, and the samples were incubated at 37 °C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory potential of prickly pears and their isolated bioactives against digestive enzymes linked to type 2 diabetes and inflammatory response

et al. 2019

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BACKGROUND Prickly pears are potential candidates for the development of low‐cost functional foods because they grow with low water requirements in arid regions of the world. They are sources of betalains and phenolic compounds, which have been reported to contribute to human health. The study of the biological activity of different varieties and of their isolated bioactive constitutes is fundamental in the design of functional foods. In this context, our objective is the assessment of the ability of Spanish and Mexican prickly‐pear cultivars to inhibit enzymes related to type 2 diabetes and the inflammatory response, and the contribution of their bioactive compounds to their nutra‐pharmaceutical potential. RESULTS Prickly pear peels presented the highest antioxidant activity due to their high isorhamnetin glycoside content. Isorhamnetin glycosides showed significantly higher antioxidant and anti‐inflammatory activity than aglycone, particularly isorhamnetin glucosyl‐rhamnosyl‐pentoside (IG2), which also reported antihyperglycemic activity. Morada, Vigor, and Sanguinos whole fruits exhibited moderate α‐amylase inhibition and higher α‐glucosidase inhibition, which is ideal for lowering glucose absorption in hyperglycemia management. Sanguinos peels presented the highest anti‐inflammatory activity because of their high indicaxanthin content and isorhamnetin glycoside profile. CONCLUSIONS In the design of prickly pear functional foods, technological processing should prioritize the retention or concentration of these bioactive compounds to preserve (or increase) their natural antioxidant, antihyperglycemic and anti‐inflammatory activity. Peels of red and orange varieties should be further evaluated for antioxidant and anti‐inflammatory purposes while whole fruits of red and purple varieties could be considered possible candidates for hyperglycemia management. © 2019 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Inhibitory potential of prickly pears and their isolated bioactives against digestive enzymes linked to type 2 diabetes and inflammatory response

et al. 2019

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“…Despite a lack of a large amount of scientific literature, we tried to compare our results to the others. Bralley et al [35] reported on anti-Hyal activity of the ethanol extracts of the Vitis rotundifolia . Michx peels and seeds of Early Fry (bronze) and Ison (purple) Vitis rotundifolia varieties.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Content and Pharma‐Nutrition Study on Eleutherococcus senticosus Fruits Intractum

Załuski

Olech

Galanty

et al. 2016

Oxidative Medicine and Cellular Longevity

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In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions. Eleutherococcus senticosus has been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. The Eleutherococcus senticosus fruits intractum was examined for the content of phenolic acids (LC-ESI-MS/MS), minerals (AAS), TPC, and TFC (spectrophotometric assay). The antioxidant activity was determined using free radical scavenging assay and TLC-DB-DPPH∗ dot-blot test. An anti-Hyal activity was evaluated by the spectrophotometric assay method. Cytotoxicity towards HL-60, HL-60/MX1, HL-60/MX2, CEM/C1, and CCRF/CEM leukemic cell lines was done using trypan blue test. Among eight phenolic acids, trans-caffeic acid was found in the largest amount (41.2 mg/g DE). The intractum presented a high amount of macroelements (Ca, Mg, K; 1750, 1300, and 21000 mg/kg) and microelements (Fe, Mn; 32.7, 54.3 mg/kg), respectively. The content of TPC and TFC was 130 and 92 mg/g DE, respectively. The intractum showed anti-Hyal activity (2.16–60%) and an antioxidant capacity (EC50; 52 μg/mL). The intractum most strongly inhibited the growth of HL-60, HL-60/MX1, and CCRF/CEM. A better understanding of the intractum health benefits is important in order to increase its utility and enrich dietary sources of health promoting compounds.

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“…Bralley and co-authors have tested the ethanol extract of fruit skin and seed for their inhibitory potential against hyaluronidase enzyme. In their study they observed the bronz IC 50 = 0.3 mg/mL for) and purple IC 50 = 0.6, mg/mL) mascadine seed extracts as highly potent compared to the fruit skin extracts of the two mascadine types (IC 50 = 1.0, 1.0 mg/mL respectively) [94].…”

Section: Anti-hyaluronidase Medicinal Plant Extractsmentioning

confidence: 95%

Plant Natural Products: A Promising Source of Hyaluronidase Enzyme Inhibitors

Bhatti¹,

Karim²

2021

Biochemistry

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Hyaluronidase enzyme degrades hyaluronan, the primary component of the extracellular matrix found in connective tissues animals and on the surface of certain pathogenic bacteria. The degradation of hyaluronan is linked to a wide range of physiological and pathological process. Inhibiting the hyaluronidase enzyme is thus significant as an approach to treat a variety of diseases and health conditions such as anti-fertility, anti-tumor, antimicrobial, and anti-venom/toxin agents. HAase inhibitors of different chemical types have been identified include both synthetic compounds and constituents obtained from naturally sources. Plant natural products as HAase inhibitors are unique due to their structural features and diversity. Medicinal plants have historically been used as contraceptives, antidote for snakebites and to promote wound healing. In recent years, small molecules, particularly plant natural products (alkaloids, flavonoids, polyphenol and flavonoids, triterpenes and steroids) possessing potent HAase have been discovered. A number of plant species from various families, which have folk medicinal claims for these ailments (related to hyaluronan disturbances) were scientifically proven for their potential to block HAase enzymes.

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Inhibition of Hyaluronidase Activity byVitis rotundifolia. (Muscadine) Berry Seeds and Skins

Cited by 24 publications

References 45 publications

Inhibitory potential of prickly pears and their isolated bioactives against digestive enzymes linked to type 2 diabetes and inflammatory response

Inhibitory potential of prickly pears and their isolated bioactives against digestive enzymes linked to type 2 diabetes and inflammatory response

Phytochemical Content and Pharma‐Nutrition Study on Eleutherococcus senticosus Fruits Intractum

Plant Natural Products: A Promising Source of Hyaluronidase Enzyme Inhibitors

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