Inhibition of initial adhesion of oral bacteria through a lectin from<i>Bauhinia variegata</i>L. var. variegata expressed in<i>Escherichia coli</i>

Klafke, Gabriel Baracy; Borsuk, Sibele; Gonçales, Relber Aguiar; Arruda, Francisco Vassiliepe Sousa; Carneiro, Victor Alves; Teixeira, Edson Holanda; Silva, A.L. Coelho da; Cavada, Benildo S.; Dellagostin, Odir A.; Pinto, Luciano S.

doi:10.1111/jam.12318

Cited by 28 publications

(23 citation statements)

References 47 publications

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“…Some studies demonstrated that components of the bacterial cell wall, such as lipopolysaccharides (LPS), peptidoglycans and teichuronic and teichoic acids, are responsible for the antibacterial effects of lectin (Klafke et al, 2013). In the other hand, A. jiringa seed lectin presented higher levels of antimicrobial activity against gram-positive bacteria than against gram-negative bacteria (Charungchitrak et al, 2010), demonstrating that lectin acts against all bacteria groups.…”

Section: Discussionmentioning

confidence: 99%

“…The (sugar) cores of E. coli and S. enterica, for example, are composed of the common hexoses, glucose, galactose, and N-acetylglucosamine (Klafke et al, 2013), two of which are CqLec-specific. The concentration needed for bacterial growth inhibition by CqLec is similar to those presented by Indigofera …”

Section: Discussionmentioning

confidence: 99%

“…Consequently, they are capable of inhibiting the motility and multiplication of microorganisms (Oliveira et al, 2007). The interaction between carbohydrates and lectins act in many biological processes, and intermediate a wide range of activities, including bacterial and fungal growth inhibition (Klafke et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

et al. 2015

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A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool.Keywords: antimicrobial; Chenopodium quinoa; glucose/mannose-specific; lectin; seeds. Practical Application:The purification and characterization of this novel lectin allow its study for a diversity of applications such as antibacterial, anticancer, anti-inflammatory and biotechnology and its further use as drugs for human diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

et al. 2015

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“…Studies on this subject also failed to respond effectively, and the physiological role of lectins are against the caries process. Some authors suggest various proposals regarding to this question and present meaningful data, especially with regard to the actions of lectins of higher plants Cavalcante et al, 2011;Klafke et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Plant lectins as alternative tools against bacterial biofilms

Cavalcante¹,

Firmino²,

Tajra³

et al. 2014

Afr. J. Microbiol. Res.

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Plant lectins has the ability to interfere in crucial biofilm formation aspects, playing a significant role in evaluation of patients at high and low risk of disease development. However, there is limited studies on the physiological role of lectins on bacteria living in biofilms like caries process. Thus, we aimed to provide a view of lectins biotechnological potential against bacterial biofilms development. Biofilm is a structured bacterial consortium that provides essential compounds for its survivor. This microorganism organization occurs naturally, since this arrangement increases its survival possibility. Bacterial biofilms are related to human health problems and are responsible for many infectious diseases, such as oral diseases, associated with inert surfaces, including medical devices for internal and external use. Thus, lectins are a large group of heterogeneous proteins that exhibit antibacterial activity, as well as ability to interfere with microbial biofilms formation process. The lectins ability to form complexes with microbial glycoconjugates has stimulated its application as probes to the whole cell, as well as its mutants and numerous cellular constituents and metabolites. Thus, the impact of bacterial resistance provided by biofilm formation on human health encourages researches aiming to understand biofilm mechanisms as well as strategies to eradicate or minimize these communities damages.

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“…Few novel composition found in plants are useful in the condition of cancer, immunosuppression, uncontrolled proliferation and angiogenesis [10,11,12]. Highest antioxidant potential was found in stem bark extracts of B. variegata [13,14,15]. Studies showed significant antioxidant activities of these B. variegata and suggested that it showed pronounced cytotoxic effect against DU-145, HOP-62, IGR-OV-1, MCF-7, and THP-1 human cancer cell lines with 90-99% cell growth inhibitory activity.…”

mentioning

confidence: 99%

Study of Phytochemical, Anti-Microbial, Anti-Oxidant, Phytotoxic, and Immunomodulatory Properties of Bauhinia variegata

Bhandari

Thapa²,

Niraula³

et al. 2017

JTLS

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In this paper, we investigated the anti-microbial, anti-oxidant, phytotoxic and immunomodulatory activity of plant Bauhinia variegata and also established the presence of important phytoconstituents which might signify its anticancer property. Phytochemical screening, anti-microbial assay, phytotoxicity assay, anti-oxidant assay and immunomodulatory analysis was done according to the protocol established at Department of Biotechnology, Kathmandu University and Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Pakistan. In the phytochemical screening, we observed the presence of different phytochemicals like steroids, terpenoids, flavonoids, reducing sugars and glycosides. In case of antimicrobial assay, the plant was found to be most effective against Bacillus cereus with the highest zone of inhibition (ZOI) of 12 mm and against Rhizopus with ZOI of 11 mm. During the antioxidant assay in comparison to ascorbic acid; at highest concentration, the scavenging activity shown by the plant B. variegata are 43.38% as compared to ascorbic acid 89.25%. In the immunomodulatory assay at the highest concentration i.e. 250 µg/mL, the plant showed 75.1% effect, which showed that the plant has potential anti-inflammatory potential. In phytotoxicity assay, B. variegata showed 20% phytotoxicity. B. variegata has thus been proven to be an important candidate to be used as an antimicrobial, antioxidant, anti-inflammatory, phytotoxic and anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from B. variegate is highly recommended as crude extract itself showed promising phytotoxicity and other pharmaceutical potential.

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Inhibition of initial adhesion of oral bacteria through a lectin fromBauhinia variegataL. var. variegata expressed inEscherichia coli

Cited by 28 publications

References 47 publications

Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

Plant lectins as alternative tools against bacterial biofilms

Study of Phytochemical, Anti-Microbial, Anti-Oxidant, Phytotoxic, and Immunomodulatory Properties of Bauhinia variegata

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