Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

Pompeu, Dávia Guimarães; Mattioli, Marcelo Augusto; Ribeiro, Rosy Iara Maciel de Azambuja; Gonçalves, Daniel Bonoto; Magalhães, Juliana Teixeira de; Marangoni, Sérgio; Silva, José Antônio da; Granjeiro, Paulo Afonso

doi:10.1590/1678-457x.6823

Cited by 30 publications

(21 citation statements)

References 31 publications

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“…In our work, the fractionated lectins from the tested Phaseolus vulgaris seeds demonstrated antibacterial activity against the Gram-negative bacteria Pseudomonas aeruginosa and Klebsiella pneumoniae, and Gram-positive bacteria Staphylococcus aureus (Table 2). This could be further confirmed by the results that showed antibacterial activity of lectins obtained from Indigofera heterantha legume seeds and those extracted from Chenopodium quinoa seeds [37,38].…”

Section: Discussionsupporting

confidence: 56%

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

El-S¹,

El-A²,

S³

2017

J Microb Biochem Technol

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Section: Discussionsupporting

confidence: 56%

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

El-S¹,

El-A²,

S³

2017

J Microb Biochem Technol

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“…Moreover, Pompeu et al (2015) obtained only inhibition to gram-negative bacteria using a lectin isolated from quinoa seeds. In general, the inhibition level is related to the chemical structure of the bioactive compounds and bacterial species (Landete, 2012).…”

Section: Antimicrobial Activity Of Chilean Quinoa Seeds: Microbiologimentioning

confidence: 99%

ASSESSMENT OF DIETARY FIBER, ISOFLAVONES AND PHENOLIC COMPOUNDS WITH ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF QUINOA (Chenopodium quinoa Willd.)

Vega‐Gálvez

Zura

Lutz³

et al. 2018

Chil. j. agric. anim. sci.

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RESUMENEl consumo de quinua (Chenopodium quinoa Willd.) está aumentando debido a su valor nutricional y beneficios para la salud relacionados con su capacidad antioxidante. El objetivo de presente estudio fue determinar los contenidos de fibra dietética, polifenoles, flavonoides e isoflavonas, y cómo estos compuestos contribuyen a la actividad antioxidante y antimicrobiana de seis ecotipos de quinua cultivados en tres zonas de Chile. Las muestras de quinua de la zona Norte se denominan Ancovinto y Cancosa, de la zona Centro se llaman Cáhuil y Faro, y de la zona Sur se denominan Regalona y Villarrica. Los resultados mostraron que todos los ecotipos de quinua chilena pueden considerarse buena fuente de fibra dietética (12,23 g 100 g -1 materia seca) y de polifenoles (161,32 mg de Equivalentes de Acido Gálico (EAG) 100 g -1 materia seca). Los ecotipos de la zona Norte y Centro mostraron la mayor concentración de isoflavonas. El ecotipo Cancosa, de la zona Norte, fue el que mostró el mayor contenido de flavonoides (211,06 mg Equivalentes de Catequina (ECA) 100 g -1 materia seca). Basado en el ensayo ORAC (del inglés Oxigen Radical Absorbance Capacity), los ecotipos Ancovinto, Cancosa y Faro, presentaron en promedio la mayor capacidad antioxidante (67,6 mmol de Equivalentes Trolox (ET) 100 g -1 materia seca). El ecotipo Regalona mostró la más alta actividad antimicrobiana frente a Saccharomyces cerevisiae y Listeria innocua. Los compuestos bioactivos encontrados en este estudio aportan nuevos conocimientos a la actividad antioxidante y antimicrobiana de las semillas de quinua chilena.Palabras clave: ecotipos de quinoa, actividad antioxidante, isoflavonas, actividad antimicrobiana.Chilean J. Agric. Anim. Sci., ex Agro-Ciencia (2018) 34(1):57-67 58 ABSTRACTThe consumption of quinoa (Chenopodium quinoa Willd.) has been steadily increasing due to its high nutritional value and health benefits associated with its high antioxidant capacity. The objective of this study was to determine the contents of dietary fiber, polyphenols, flavonoids and isoflavones, and how they contribute to the antioxidant and antimicrobial activity of six ecotypes of quinoa cultivated in three different zones of Chile. The ecotypes studied were: Ancovinto and Cancosa (Northern zone), Cáhuil and Faro (Central zone), and Regalona and Villarrica (Southern zone). The results indicate that all Chilean quinoa ecotypes could be considered as good sources of dietary fiber (12.23 g 100 g -1 dry matter) and polyphenols (161.32 mg Gallic Acid Equivalents (GAE) 100 g -1 dry matter). The North and Central ecotypes exhibited the highest isoflavone concentration. The Northern Cancosa was the ecotype that showed the highest flavonoid content (211.06 mg Catechin Equivalents (CAE) 100 g -1 dry matter). Based on the ORAC (Oxygen Radical Absorbance Capacity) assay, Ancovinto, Cancosa and Faro presented the highest antioxidant capacity (67.6 mmol Trolox Equivalents (TE) 100 g -1 dry matter). Regarding antimicrobial activity, Regalona ecotype showed the best performanc...

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“…Lectins derived from different plant sources have shown strong antiviral activity for various plant viruses, so the present study focuses on isolation of CML protein from Chenopodium murale, which belongs to family Chenopodiaceae. Lectin isolated from Chenopodum quinoa have two subunits of 35kDa and 25kDa which have the ability to hemagglutinate the rabbit erythrocytes similar as described by [18]. Lectins are known to induce cytotoxicity in cancer cells by interacting with them or their receptors to produce necrosis, autophagy, apoptosis or inhibit cell growth [9].…”

Section: Discussionmentioning

confidence: 97%

In-vitro Analysis of Chenopodium murale extract for resistance against potato virus Y

Bilal¹

2019

PAB

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Potato (Solanum tuberosum) is one of the largely consumed staple food crop of the world, including Pakistan. In Pakistan, potato shares a large percentage in country's agriculture GDP. In Pakistan majority of good quality virus free seed potato is imported by multinational and local seed companies. This costs a huge burden on country's economy. Potato Virus Y (PVY) belongs to Potyviridae family, severely damaging leaves of the Solanaceae family including potato. Plant lectins are the potential proteins that specifically bound and cross linked with carbohydrates and target glycans present on the cell surface of viruses through a pathway termed as lectin activation. The present in-vitro study shows the effect of lectin extracted from the seeds of Chenopodium murale against the downregulation of the CP (coat protein) gene of PVY. Stable interaction was found between PVY coat protein and CML insilico. Full length CP gene from PVY virus was amplified and cloned in mammalian expression vector. CML protein was purified to sub fractions through anion exchange chromatography on SP-sepharose gel. The non-cytotoxic effect and safe limit of CML extract was indicated by CC50 value. The hemagglutination activity of CML against the rabbit erythrocytes was revealed to be 35 µg/ml. The real-time PCR revealed the anti-PVY activity of CML extract. CML extract in concentration of 30, 60 and 90 µg/ml was found effective against PVY CP gene in comparison with control.

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Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

Cited by 30 publications

References 31 publications

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

Antimicrobial Activities of Lectins Extracted from Some Cultivars of Phaseolus vulgaris Seeds

ASSESSMENT OF DIETARY FIBER, ISOFLAVONES AND PHENOLIC COMPOUNDS WITH ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF QUINOA (Chenopodium quinoa Willd.)

In-vitro Analysis of Chenopodium murale extract for resistance against potato virus Y

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