Inhibition of Interleukin-1β Production and Gene Expression in Human Gingival Fibroblasts by Low-energy Laser Irradiation

Abstract

Human gingival fibroblast (hGF) cells reside in gingival tissues which are challenged frequently by oral bacteria. Lipopolysaccharide (LPS) from periodontal pathogens can penetrate gingival tissues and stimulate the production of interleukin-1beta (IL-1beta), which has been implicated in inflammation and bone resorption. The anti-inflammatory effects of low-energy laser irradiation have been reported, but the mechanisms of this biostimulatory effect have not been fully elucidated. Primary cultured hGF cells were challenged with LPS isolated from Campylobacter rectus, a known periodontal disease-associated pathogen, and irradiated by a Ga-Al-As diode low-energy laser (830 nm, 3.95-7.90 J/cm2). The hGF cells cultured medium showed a marked elevation of IL-1beta production by LPS, which was significantly inhibited by laser irradiation in a dose-dependent manner. By reverse transcription-polymerase chain reaction (RT-PCR) analysis, this inhibitory effect was involved in the reduction of IL-1beta mRNA levels but not that of the IL-1beta converting enzyme.

Anti-inflammatory activities of light emitting diode irradiation on collagen-induced arthritis in mice (a secondary publication)

“…These promote the expression of receptor activator of nuclear factor kappa-B ligand (RANKL) in synovial fibroblasts, the differentiation of osteoclasts and also induce MMP-3 production, all of which play parts in the destruction of the bone and joint [24][25][26][27] . In a previous in vitro study, the first author has demonstrated that Ga-Al-As diode laser irradiation (Panalas-1000: wavelength 830 nm, total energy density 7.9 J/ cm 2 ) significantly suppressed the production of PGE2, IL-1ß and PA in LPS stimulated human gingival fibroblast cell cultures [28][29][30] . Joint cartilage is composed of mainly type 2 collagen and chondroitin sulfate along with other extra-cellular matrix substances such as hyaluronic acid.…”

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

“…In the literature is reported that LLLT acts by reducing the inflammation process and accelerating wound healing in rats (29,54). Several authors showed that laser irradiation caused inhibition of PGE 2 through reduction of COX-2 mRNA levels (22,(55)(56)(57). In addition, low-level laser irradiation significantly inhibited the gene expression of IL-1β and IFN-γ (55,58).…”

“…In addition, low-level laser irradiation significantly inhibited the gene expression of IL-1β and IFN-γ (55,58). IL-1β, PGE 2 , and IFN-γ are involved in different immune responses and in the acute phase of inflammatory processes (56,59). Also, IFN-γ is an important macrophage activator and plays an important role in the inflammatory process (59).…”

Low level 809‐nm diode laser‐induced in vitro stimulation of the proliferation of human gingival fibroblasts

“…A 830-nm GaAlAs-laser irradiation inhibited prostaglandin E2 and IL-1b production and gene expression which might be of therapeutic benefit against the aggravation of gingivitis and periodontitis that accompanies bacterial infection [32,33]. Takema et al, 2000 described an 830-nm semiconductor laser-induced inhibition of the plasminogen activator (PA)-plasmin proteolytic system [34].…”