Inhibition of MAP kinases by crude extract and pure compound isolated from Commiphora mukul leads to down regulation of TNF-α, IL-1β and IL-2

Manjula, Nemali; Balakrishnan, Gayathri; Vinaykumar, Kontham Sanathkumar; Shankernarayanan, N.P.; Vishwakarma, Ram A.; Balakrishnan, Arun

doi:10.1016/j.intimp.2005.07.001

Cited by 52 publications

(26 citation statements)

References 42 publications

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“…Interestingly, the methanol extract of H. ovalis inhibited the PBMC proliferation. Organic extracts from different plants were reported to inhibit proliferation of PBMC (Manjula et al, 2006;Gayathri et al, 2007;Wu et al, 2007). In our study, inhibition of proliferation of mitogen-induced PBMC in the presence of added methanol extract was observed.…”

Section: Discussionmentioning

confidence: 99%

Seagrass as a potential source of natural antioxidant and anti-inflammatory agents

Yuvaraj

Kanmani

Satishkumar

et al. 2011

Pharmaceutical Biology

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Section: Discussionmentioning

confidence: 99%

Seagrass as a potential source of natural antioxidant and anti-inflammatory agents

Yuvaraj

Kanmani

Satishkumar

et al. 2011

Pharmaceutical Biology

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“…5D). Because PI3K and p38MAPK are known to play a role in IL-1b production (36)(37)(38)(39)(40) and IRAK can also activate p38MAPK (9), we evaluated PMA-induced IL-1b production in the presence of SB 202190 (p38MAPK INH) and LY294002 (PI3K INH) (Fig. 5D).…”

Section: Pma Induces Il-1b Production In a Jnk-dependent But Nf-kb-inmentioning

confidence: 99%

IL-1R–Associated Kinase-1 Mediates Protein Kinase Cδ-Induced IL-1β Production in Monocytes

Tiwari

Singh

et al. 2011

The Journal of Immunology

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The role of IL-1R–associated kinase (IRAK)1 and its interaction with protein kinase C (PKC)δ in monocytes to regulate IL-1β production has not been reported so far. The present study thus investigates such mechanisms in the THP1 cell line and human monocytes. PMA treatment to THP1 cells induced CD11b, TLR2, TLR4, CD36, IRAK1, IRAK3, and IRAK4 expression, IRAK1 kinase activity, PKCδ and JNK phosphorylation, AP-1 and NF-κB activation, and secretory IL-1β production. Moreover, PMA-induced IL-1β production was significantly reduced in the presence of TLR2, TLR4, and CD11b Abs. Rottlerin, a PKCδ-specific inhibitor, significantly reduced PMA-induced IL-1β production as well as CD11b, TLR2 expression, and IRAK1–JNK activation. In PKCδ wild-type overexpressing THP1 cells, IRAK1 kinase activity and IL-1β production were significantly augmented, whereas recombinant inactive PKCδ and PKCδ small interfering RNA significantly inhibited basal and PMA-induced IRAK1 activation and IL-1β production. Endogenous PKCδ–IRAK1 interaction was observed in quiescent cells, and this interaction was regulated by PMA. IRAK1/4 inhibitors, their small interfering RNAs, and JNK inhibitor also attenuated PMA-induced IL-1β production. NF-κB activation inhibitor and SN50 peptide inhibitor, however, failed to affect PMA-induced IL-1β production. A similar role of IRAK1 in IL-1β production and its regulation by PKCδ was evident in the primary human monocytes, thus signifying the importance of our finding. To our knowledge, the results obtained demonstrate for the first time that IRAK1 and PKCδ functionally interact to regulate IL-1β production in monocytic cells. A novel mechanism of IL-1β production that involves TLR2, CD11b, and the PKCδ/IRAK1/JNK/AP-1 axis is thus being proposed.

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“…The dried powder (50 g) was extracted with hexane, chloroform, ethyl acetate, and 70% methanol (Merck, Germany) separately at room temperature and the solvents from the combined extracts were evaporated by vacuum rotary evaporator. The extraction process yielded in hexane 3.5 g, chloroform 5.2 g, ethyl acetate 5.6 g, and methanol 6.1 g extracts (Manjula et al, 2006). The solvents used in this study were of analytical grade and the purity of the solvents was over 99.5%.…”

Section: Preparation Of Leaf Extractsmentioning

confidence: 99%

Antibacterial activity of leaf extracts ofPongamia pinnatafrom India

Bajpai

Rahman

Shukla

et al. 2009

Pharmaceutical Biology

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Inhibition of MAP kinases by crude extract and pure compound isolated from Commiphora mukul leads to down regulation of TNF-α, IL-1β and IL-2

Cited by 52 publications

References 42 publications

Seagrass as a potential source of natural antioxidant and anti-inflammatory agents

Seagrass as a potential source of natural antioxidant and anti-inflammatory agents

IL-1R–Associated Kinase-1 Mediates Protein Kinase Cδ-Induced IL-1β Production in Monocytes

Antibacterial activity of leaf extracts ofPongamia pinnatafrom India

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