Inhibition Of Microsomal Lipid Peroxidation And Protein Oxidation By Extracts From Plants Used In Bamun Folk Medicine (Cameroon) Against Hepatitis

Abstract

The antioxidant activities of 53 medicinal plants used in Bamun Folk Medicine for the management of jaundice and hepatitis were investigated. The studies were done using rat hepatic microsomes for lipid peroxidation and bovine serum albumin (BSA) for carbonyl group formation. Silymarine was used as reference compound. Fifteen different extracts were effective at a dose of 200µg/ml in both experiments. Specifically, 25 extracts inhibited lipid peroxidation initiated non-enzymatically by ascorbic acid while 18 inhibited peroxidation as determined by reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH). The inhibitory concentration 50 (IC 50 ) of 23 different plant extracts was lower than 200µg/ml in the microsomal lipid peroxidation inhibition study. Fifteen of the 23 extracts were active in preventing protein oxidation by inhibiting the formation of the carbonyl group on BSA with an IC 50 value less than 200µg/ ml. The results suggest that the antioxidant activity of the extracts, may be due to their ability to scavenge free radicals involved in microsomal lipid peroxidation or in protein oxidation. These biochemical processes are involved in the aetiology of toxic hepatitis.

The anti-arthritic and anti-inflammatory activities of aqueous extract powder bark of Anthocleista schweinfurthii Gilg (Loganiaceae)

“…We reported the identification of isocyanate, Soscoparin, biphenyltriol, methoxy-flavone, glycoside and flavonoids in the ASE. This result confirms literature review which revealed that Antocleista genus is a rich source of biocompounds such as alkaloids, xanthone, scoiridoids, and terpene 24 , contradicting in other hands to some author who reported that reducing sugar and glycosides were found to be absent in both the leaf and the stem-bark of the Anthocleista species 25,26 . Inflammation is induced by several pathway, one of the most pathway, it is initiated by the action of lipoxygenase on arachidonic acid producing trioxilins and leukotriene 29 .…”

African natural products with potential antioxidants and hepatoprotectives properties: a review

“…Njayou et al [117], evaluated the hepatoprotective effect of fifty four Cameroonian plants extracts against Fe (II)-Ascorbate induced microsomal lipid peroxidationin rat liver. Only 15 plants extract inhibiting oxidation phenomena with percentage inhibition of > 50 at 200 μg/mL were considered as possessing a high lipid oxidation inhibitory potential.…”

“…But our close analysis of the reports seem to suggest that Combretum apiculatum, Telfaria occidentalis, Acalypha racemosa, Garcinia lucida, Xeoderris sthulmannii, Clausena anisata, Harpephyllum caffrum, Ceratotheca sesamoides, Camellia sinensis, Cyathea dregei, Harpephyllum caffrum, Aspalathus linearis were the most active ROS-detoxifying plant extracts from African flora. The best ROS-detoxifying phytochemicals were moracin T, U, S and R (84-87), oleanolic acid (54), 5,7,4′-trihydroxy-3,8,3′,5′-tetramethoxyflavone (89), 5,7,3′-trihydroxy-3,8,4′,5′-trimethoxyflavone (88), luteolin (3′,4′, 5,7-tetrahydroxy flavone) (117) and genistein (4′,5,7-trihydroxyisoflavone) (116). It is hoped that pertinent scientist and stakeholders will look further into some of these plants and compounds for detailed authentification and subsequent commercialization.…”

Effects of the stem barks aqueous extract of Anthocleista schweinfurthii Gilg (Loganiaceae) on acute and chronic gastric ulcers rats models

“…Phytochemical studies of A. schweinfurthii have revealed the presence of flavonoids, polyphenols, tannins and leucoanthocyans (Njayou et al, 2000). Polyphenols compounds protect the gastrointestinal mucosa from lesions produced by various experimental ulcer models and against different necrotic agents.…”