Inhibition of miRNA-221 Suppresses the Airway Inflammation in Asthma

Abstract

This study investigated the expression of miRNA-221 in asthmatics in order to determine whether miRNA-221 plays a role in the development of asthma. Real-time PCR was used to detect the miRNA-221 in both asthmatic and control subjects. In addition, airway inflammation was evaluated by cell counting and tissue biopsy in the OVA-induced murine asthma model. miRNA-221 was differentially expressed in asthmatics and control subjects, and miRNA-221 blockade resulted in a reduction of airway inflammation in the OVA-induced murine asthma model. We conclude that miRNA-221 participates in the pathogenesis of asthma and that inhibition of miRNA-221 suppresses airway inflammation in asthmatics.

Small interfering RNA directed against microRNA‑155 delivered by a lentiviral vector attenuates asthmatic features in a mouse model of allergic asthma

“…The role of miRNAs in the pathogenesis of allergic asthma has been widely studied since 2007 (7)(8)(9)12,16,29) and therapies that target miRNAs were considered to be important for further study (30,31). The results of the present study indicate that miR-155 is associated with the development of allergic airway inflammation; miR-155 expression was significantly higher in the lungs of mice with asthma compared with normal controls.…”

Differential Expression of MicroRNAs in Chronic Obstructive Pulmonary Disease

“…It is believed that miR-17-92 cluster (take part in 13q31.3) expressions of pulmonary to be high during embryonic development and low in adulthood, has a role in the regulation of pulmonary development [11,15]. Qin et al stated that miRNA-221 expression exposed to up-regulation in patients with asthma and that it could be a functional regulator for asthma pathogenesis [6]. Chen et al stated that miR-146a expression was down-regulated in the inflammatory process of pulmonary diseases, and Bockmeyer et al stated that there was an increase in miR-143/145 expression [12,16].…”

“…Many studies make us think that miRNAs act in pulmonary diseases including airway diseases like COPD [6,7]. However, there are very few studies that reveal the relation between COPD and miRNAs.…”

Epigenetic targets for novel therapies of lung diseases

“…It should be noted there are contradictory results concerning contributions of let-7 and miR-21 to asthma pathogenesis (Collison et al, 2011;Kumar et al, 2011;Lu et al, 2009; Polikepahad et al, 2010). Animal studies are supported by profiling studies identifying differentially expressed miRNAs in tissues, cells and exosomes from human subjects with asthma (Jardim et al, 2012; Jude et al, 2012; Levanen et al, 2013; Liu et al, 2012; Nakano et al, 2013; Perry et al, 2014; Qin et al, 2012; Solberg et al, 2012; Suojalehto et al, 2014). The evidence clearly shows that miRNA expression changes in asthma which may cause disease-related alterations in cell phenotypes that contribute to tissue remodeling and inflammation.…”

“…Inhibition of miR-145 alleviated the hallmark features of allergic inflammation such as eosinophilic inflammation, mucus hyper-secretion, Th2 differentiation and airway hyperreactivity (Collison et al, 2011; Mattes et al, 2009). Inhibition of miR-221 reduced airway inflammation in a mouse model (Qin et al, 2012). MiR-221 regulates mast cell activation, which is consistent with reduced inflammation in mice treated with a miR-221 inhibitor (Mayoral et al, 2011).…”