Inhibition of multixenobiotic resistance transporters (MXR) by silver nanoparticles and ions in vitro and in Daphnia magna

Differential Action of Silver Nanoparticles on ABCB1 (MDR1) and ABCC1 (MRP1) Activity in Mammalian Cell Lines

“…Interestingly, only AgNPs increased the calcein accumulation in wild type MDCKII cell line, that might suggest the direct interaction of NPs with proteins, rather than the indirect action through thiol group inhibition or mitochondrial interactions. The authors confirmed the observed phenomenon in an in vivo model using Daphnia magna, where an increased accumulation of calcein was observed after 60 min exposure to AgNPs [56]. The inhibition of ABCB1 by citrate-modified AgNP function was also observed in MCF-7 and MCF-7/KCR (cells with ABCB1 overexpression).…”

Ecotoxicological Effects of Transformed Silver and Titanium Dioxide Nanoparticles in the Effluent from a Lab-Scale Wastewater Treatment System

“…The multixenobiotic resistance (MXR) mechanism mediated by ATP binding cassette transporters is an important mechanism of defense against xenobiotics, which functions by extruding them or their metabolites out of the cell. The transporters are localized in tissues with a barrier function or involved in secretion and absorption, they transport a wide variety of compounds across cell membranes and it has recently been shown that NPs, including Ag NPs, can interfere with the MXR system. , Due to their importance in cellular defense against xenobiotics, the multixenobiotic resistance genes ABCB1, ABCC1, ABBC2 were also investigated in the current study. Exposure to the effluents led to increased mRNA levels of ABCB1, ABCC1, and ABCC2 transporters, with ABCB1 (the most responsive) exhibiting increased expression levels in response to effluents from weeks 1–3 (3.4-fold increase upon exposure to effluent week 2) (SI Figure S7).…”

Metabolic alterations in alga Chlamydomonas reinhardtii exposed to nTiO2 materials

“…Among the dysregulated ABC transporters, the multidrug resistance-associated protein 12 (Cre10.g458450.t1.3, MRP12, FC 7.38, FDR 6.40 × 10 −10 ) and P-loop containing nucleoside triphosphate hydrolase superfamily protein (Cre10.g444700.t1.1, FC 7.96, FDR 6.71 × 10 −11 ) were strongly up-regulated suggesting the involvement of the cellular mechanism in metal detoxification in algal. 100,101 Several zinc (Zn)-regulated transporters, iron (Fe)regulated transporter-like proteins (ZIP), transporters of Fe and Zn, and Zn transporters were also up-regulated. These regulations suggest the impact of A5 on the global nutrition of the microalgal.…”