Inhibition of Nuclear Factor κB Activation and Cyclooxygenase-2 Expression by Aqueous Extracts of Hispanic Medicinal Herbs

Orlando, Robert A.; Gonzales, Amanda; Hunsaker, Lucy A.; Franco, Carolina; Royer, Robert E.; Jagt, David L. Vander; Jagt, Dorothy J. Vander

doi:10.1089/jmf.2009.1128

Cited by 19 publications

(16 citation statements)

References 37 publications

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“…20 NSAIDs are used for treating inflammatory diseases. 21,22 The results of this study showed that intraperitoneal injection of LPS could cause alteration in hematological parameters, as the level of WBC in LPS group significantly reduced, and pretreatment with diclofenac could not restore these parameters to normal levels ( Table 1). The post-endotoxin leukopenia mainly resulted from a decrease in circulating neutrophil count.…”

Section: Resultsmentioning

confidence: 86%

Effect of Diclofenac on Hematological Parameters and Inflammatory Markers in Rat after Injection of Escherichia coli Lipopolysaccharide

Yektaseresht¹,

Jalali²,

Khadjeh³

2018

Int J Enteric Pathog

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Background Lipopolysaccharides (LPSs) are found in the outer membranes of gram-negative bacteria, such as Salmonella and Escherichia coli. 1 Injection of LPS evokes proinflammatory response, and in the blood may result in fever, shock, organ failure, and death. 2 LPS is used in the modeling of endotoxemia in experimental models. 3 Two cyclooxygenase enzymes are known. Cyclooxygenase-2 (COX-2) regulates release of prostaglandins important for the inflammatory response. 4 Expression of COX-2 is increased after exposure to LPS. 5 Monitoring the serum levels of cytokines such as interlukin-1 (IL-1), interlukin-6 (IL-6), or tumor necrosis factor-α (TNF-α) may be an effective method for the evaluation of inflammatory response and early detection of acute phase reactions. 6-8 Pro-inflammatory cytokines, such as TNF-α and IL-6 were induced by endotoxins like LPS, leading to inflammation and coagulation cascades. These cytokines may trigger the expression of tissue factors in monocytes and endothelial cells. 9 C-reactive protein (CRP) is produced by the liver in response to inflammation regulated by IL-6, IL-1, and TNF-α. 10 IL-6in LPS challenge and inflammation induces weight loss, hypoglycemia and fibrinogen production. 11 Nonsteroidal anti-inflammatory drugs (NSAIDs) are used as analgesics, anti-inflammatory drugs, and antipyretics. 12 NSAIDs block the action of COX, thereby reducing symptoms of inflammation. 13 Ruetten and Thiemermann in a recent study demonstrated that the expression of COX-2 caused by LPS in the rat was prevented by dexamethasone. 14 Yazar et al showed that flunixin meglumine inhibited the increase of inflammatory cytokine levels (TNFα and IL-1) in LPSinduced endotoxic mice. 15 Diclofenac, as an NSAID, has been widely used as the anti-inflammatory, analgesic, and antipyretic drug. The potency of diclofenac is substantially greater than that

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Section: Resultsmentioning

confidence: 86%

Effect of Diclofenac on Hematological Parameters and Inflammatory Markers in Rat after Injection of Escherichia coli Lipopolysaccharide

Yektaseresht¹,

Jalali²,

Khadjeh³

2018

Int J Enteric Pathog

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“…Conventional analgesics play an important role in pain therapy, but they always cause kinds of adverse effects during clinic use [2]. The same as the analgesics, nonsteroidal anti-inflammatory drugs (NSAIDs) are the primary therapy for diseases with a chronic inflammatory response, but long-term use often causes severe side effects, including cardiovascular and gastrointestinal complications that limit their development [3, 4]. Therefore, the research for new analgesic and anti-inflammation agents are critically needed.…”

Section: Introductionmentioning

confidence: 99%

Analgesic and Anti‐Inflammatory Activities of Resveratrol through Classic Models in Mice and Rats

Wang

Song

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Background. Inflammation and pain are closely related to humans' and animals' health. Resveratrol (RSV) is a natural compound with various biological activities. The current study is aimed to evaluate the analgesic and anti-inflammatory activities of RSV in vivo. Materials and Methods. The analgesic effects were assessed by the acetic acid-induced writhing and hot plate tests. The anti-inflammatory effects were determined using the xylene-induced mouse ear oedema, the acetic acid-induced rat pleurisy, and carrageenan-induced rat synovitis tests, respectively. Results. The analgesic results showed that RSV could significantly inhibit the number of writhes and improve the time and pain threshold of mice standing on hot plate. The anti-inflammatory results showed that RSV could inhibit the ear oedema of mice. In acetic acid-induced pleurisy test, RSV could significantly inhibit the WBC and pleurisy exudates, could decrease the production of NO, and elevate the activity of SOD in serum. In carrageenan-induced synovitis test, RSV could reduce the content of MDA and elevate the T-SOD activity in serum; RSV could inhibit the expressions of TP, PGE2, NO, and MDA. Conclusion. Shortly, these results indicated that RSV had potent analgesic and anti-inflammatory activities and could be a potential new drug candidate for the treatment of inflammation and pain.

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“…Salicyclic acid and glucocorticoid may inhibit the activation of NF-κB or COX-2. 19,20 Folic acid (FA) supplementation during pregnancy reduces the risk of neural tube defects, but it may be used to prevent preterm labor and intrauterine growth restriction (IUGR). FA significantly reduced interleukin-6 and keratinocyte-derived cytokine in the amniotic fluid of LPS-treated mice.…”

Section: Discussionmentioning

confidence: 99%

Role of nuclear factor kappa beta, tumor necrosis factor α, and cyclooxygenase-2 in preterm labor

et al. 2015

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AbstrakLatar belakang: Jalur aktivasi tumor necrosis factor alpha (TNFα), nuclear factor κB (NF-κB) dan cyclooxygenase-2 (COX-2) dalam melepaskan prostaglandin merupakan hal penting dalam proses persalinan sebagai patogenesis persalinan prematur. Penelitian ini bertujuan membandingkan perbedaan rerata ekspresi TNFα, NF-κB dan COX-2 antara persalinan prematur dengan persalinan normal serta mengetahui korelasi antara ekspresi NF-κB, TNFα dan COX-2 dalam persalinan prematur.Metode: Studi kasus-kontrol dilakukan di RS Arifin Achmad, Pekanbaru sejak Mei 2013 hingga Februari 2014. Sebanyak 30 pasien dengan persalinan prematur sebagai kasus dan 30 pasien dengan persalinan normal sebagai kontrol. Seluruh subjek mempunyai paritas maksimal 3 orang, dengan batasan umur 35 tahun, dan persalinan spontan pada kedua kelompok. Jaringan plasenta diperoleh dari seluruh subjek dan dilakukan pewarnaan hematoksilin eosin. Ekspresi TNFα, NF-κB, dan COX-2 dalam jaringan tersebut dinilai dengan pewarnaan imunohistokimia dengan menghitung persentasi sel yang terwarnai oleh dua ahli. Ekspresi TNFα, NF-κB, dan COX-2 antara kelompok dan kontrol dibandingkan menggunakan uji-t dan korelasi diselidiki dengan koefisien korelasi Pearson.Hasil: Rerata (lebih tinggi secara bermakna pada persalinan prematur dibanding persalinan matur. Selain itu, terdapat korelasi yang bermakna antara ekspresi TNFα dan NF-κB pada persalinan prematur (r = 0,385; p = 0,036), dan tidak ditemukan korelasi antara NF-κB dan COX-2 (p = 0,982).Kesimpulan: Tingginya ekspresi TNFα, NF-κB, dan COX-2 pada kelompok persalinan prematur menunjukkan perannya terhadap terjadinya persalinan prematur. Tingginya ekspresi TNFα menunjukan bahwa infeksi merupakan penyebab tertinggi persalinan prematur. Hal ini dibuktikan dengan peningkatan aktivasi NF-kB akan meningkatkan COX-2 dan kemudian meningkatkan prostaglandin sehingga terjadi persalinan prematur. AbstractBackground: The pathway of tumor necrosis factor alpha (TNFα), nuclear factor kappa beta (NF-κB), and cyclooxygenase-2 (COX-2) activation in releasing prostaglandins is suggested to be crucial for initiating labor in the pathogenesis of preterm labor. The aim of the study was to know whether there were mean differences of NF-κB, TNFα, and COX-2 expressions between preterm and term labor and also to know the correlation among them in preterm labor. in Arifin Achmad Hospital, Pekanbaru. There were 30 subjects with preterm labor as cases and 30 with normal labor as controls. All subjects had singleton gestation with maximum parity was three, age limit of 35 year-old, and spontaneous labor in both groups. Placental tissue was collected from all subjects and evaluated with hematoxylin eosin staining. The expressions of TNFα, NF-κB, and COX-2 in the tissue were assessed with immunohistochemical staining by counting the percentage of smeared cells by two experts. The expressions of TNFα, NF-κB, and COX-2 between case and control were compared using t-test and the correlation was analyzed with Pearson correlation coefficient.Results: Mea...

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Inhibition of Nuclear Factor κB Activation and Cyclooxygenase-2 Expression by Aqueous Extracts of Hispanic Medicinal Herbs

Cited by 19 publications

References 37 publications

Effect of Diclofenac on Hematological Parameters and Inflammatory Markers in Rat after Injection of Escherichia coli Lipopolysaccharide

Effect of Diclofenac on Hematological Parameters and Inflammatory Markers in Rat after Injection of Escherichia coli Lipopolysaccharide

Analgesic and Anti‐Inflammatory Activities of Resveratrol through Classic Models in Mice and Rats

Role of nuclear factor kappa beta, tumor necrosis factor α, and cyclooxygenase-2 in preterm labor

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