Inhibition of nuclear factor-κB augments antitumor activity of adenovirus-mediated melanoma differentiation-associated gene-7 against lung cancer cells via mitogen-activated protein kinase kinase kinase 1 activation

Oida, Yasuhisa; Gopalan, Began; Miyahara, Ryoji; Branch, Cynthia D.; Chiao, Paul J.; Chada, Sunil; Ramesh, Rajagopal

doi:10.1158/1535-7163.mct-06-0374

Cited by 11 publications

(5 citation statements)

References 39 publications

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“…As a result, patients with lung cancer may receive an ineffective treatment for extended periods of time. Another major clinical problem is the inability to demonstrate whether the EGFR-targeted inhibitors specifically targeted the lung tumors to produce the desired therapeutic effect [103]. …”

Section: Signaling Pathways In Human Pancreatic Cancersmentioning

confidence: 99%

Fabrication of gold nanoparticles for targeted therapy in pancreatic cancer

Patra

Bhattacharya

Mukhopadhyay

et al. 2010

Advanced Drug Delivery Reviews

419

264

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The targeted delivery of a drug should result in enhanced therapeutic efficacy with low to minimal side effects. This is a widely accepted concept, but limited in application due to lack of available technologies and process of validation. Biomedical nanotechnology can play an important role in this respect. Biomedical nanotechnology is a burgeoning field with myriads of opportunities and possibilities for advancing medical science and disease treatment. Cancer nanotechnology (1-100 nm size range) is expected to change the very foundations of cancer treatment, diagnosis and detection. Nanomaterials, especially gold nanoparticles (AuNPs) have unique physicochemical properties, such as ultra small size, large surface area to mass ratio, and high surface reactivity, presence of surface plasmon resonance (SPR) bands, biocompatibility and ease of surface functionalization. In this review, we will discuss how the unique physico-chemical properties of gold nanoparticles may be utilized for targeted drug delivery in pancreatic cancer leading to increased efficacy of traditional chemotherapeutics.

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Section: Signaling Pathways In Human Pancreatic Cancersmentioning

confidence: 99%

Fabrication of gold nanoparticles for targeted therapy in pancreatic cancer

Patra

Bhattacharya

Mukhopadhyay

et al. 2010

Advanced Drug Delivery Reviews

419

264

View full text Add to dashboard Cite

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“…Zhou et al [25] reported on the over expression of NF-kB and TNF-α is increased in breast cancer. Yasuhisa Oida et al [26] showed the findings about the NF-kB activation induced by Ad-mda7 in lung cancer, it shows hindrance of NF-kB expression enhanced in killing of tumor cell. Similarly, Birsu Cincin et al [27] revealed a study on hesperdidin had a greater suppressive on A549 and NCI -H358 cells.…”

Section: Nf-kb Expression In A549 Cellsmentioning

confidence: 99%

Mechanism underlying the inhibitory effect of Elaeagnus Conferta Roxb on TNF –α induced NF- kB nuclear translocation in non- small lung cancer A549 cell line using Flow cytometry

Lalitha¹,

Nazeema²

2020

IJPSR

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Our study examined for the inhibitory effect of ethanolic extract of Eleagnus Conferta Roxb leaves on TNF –α induced NF- kB nuclear translocation in lung cancer A549 cell line using flow cytometry. Apoptosis also studied to know about the antiproliferative and anticancer effects. However, our results revealed in apoptosis, Elaeagnus conferta Roxb leaves showed (33.54%) increased in proportion of cells. In the study of pre-treatment of A549 cells with Elaeagnus conferta Roxb leaves followed by TNF- α caused the increased proportion of cells (20.78%) at apoptosis induced cell death, which was statistically significant (p< 0.001). The untreated A549 cells had minimal NF-kB expression (0.25± 0.01%). However, the approach of A549 cells with Elaeagnus conferta Roxb had induced NF-kB production many fold (11.50± 1.05%). Therefore, we conclude our study was proved the impact of Elaeagnus conferta Roxb leaves inhibit the cellular growth of NSCLC-A549 cell line and induces apoptosis. Hence, from our findings, we proved this plant has anticancer activity, further feasibly taken for drug formulation.

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“…At 72 hours after treatment, the cells were harvested by trypsinization and cell viability determined by trypan blue staining as previously described. 16,19 The viability of the untreated cells (the control) was considered 100%. The number of viable cells per treatment was determined and expressed as percentage surviving compared with untreated control cells.…”

Section: Egfr-targeted 225-nanoparticles Induce Dna Damagementioning

confidence: 99%

“…16,19 Total cellular proteins (50 µg) were applied and separated by SDS (sodium dodecyl sulfate) 7.5%-12.5% PAGE (polyacrylamide gel electrophoresis) and transferred electrophoretically to PVDF-Plus membranes (Micron Separations, Westborough, MA, USA). Primary antibodies against PARP (1:1,000 dilution), phospho-Cdc2 (Tyr15; 1:1,000 dilution), phospho-histone H3 (Ser10; D2C8; 1:1,000 dilution; Cell Signaling Technology, Beverly, MA, USA), anti-phospho/total-EGFR antibody (1:500 dilution; Santa Cruz Biotechnology Inc., Dallas, TX, USA), phosphohistone H2AX (Ser139) (1:2,500 dilution; EMD Millipore, Billerica, MA, USA), BRCA1 (1:5,000 dilution; Novus, Littleton, CO, USA), Chk1 (G-4), and Cdc2 p34 (1:10,000 dilution; Santa Cruz Biotechnology) were purchased and used.…”

Section: Western Blottingmentioning

confidence: 99%

“…The cells were subsequently stained with propidium iodide solution (Hoffman-La Roche Ltd., Basel, Switzerland) and analyzed with a FACScan flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) as previously described. 16,19 The number of cells in the various phases of the cell cycle was determined, and the data were analyzed using the FlowJo software (Tree Star Inc., Ashland, OR, USA). 16 …”

Section: Flow Cytometrymentioning

confidence: 99%

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EGFR-targeted plasmonic magnetic nanoparticles suppress lung tumor growth by abrogating G2/M cell-cycle arrest and inducing DNA damage

Kuroda¹,

Tam²,

Roth³

et al. 2014

IJN

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Background We have previously demonstrated the epidermal growth factor receptor (EGFR)-targeted hybrid plasmonic magnetic nanoparticles (225-NP) produce a therapeutic effect in human lung cancer cell lines in vitro. In the present study, we investigated the molecular mechanism of 225-NP-mediated antitumor activity both in vitro and in vivo using the EGFR-mutant HCC827 cell line. Methods The growth inhibitory effect of 225-NP on lung tumor cells was determined by cell viability and cell-cycle analysis. Protein expression related to autophagy, apoptosis, and DNA-damage were determined by Western blotting and immunofluorescence. An in vivo efficacy study was conducted using a human lung tumor xenograft mouse model. Results The 225-NP treatment markedly reduced tumor cell viability at 72 hours compared with the cell viability in control treatment groups. Cell-cycle analysis showed the percentage of cells in the G2/M phase was reduced when treated with 225-NP, with a concomitant increase in the number of cells in Sub-G1 phase, indicative of cell death. Western blotting showed LC3B and PARP cleavage, indicating 225-NP-treatment activated both autophagy- and apoptosis-mediated cell death. The 225-NP strongly induced γH2AX and phosphorylated histone H3, markers indicative of DNA damage and mitosis, respectively. Additionally, significant γH2AX foci formation was observed in 225-NP-treated cells compared with control treatment groups, suggesting 225-NP induced cell death by triggering DNA damage. The 225-NP-mediated DNA damage involved abrogation of the G2/M checkpoint by inhibiting BRCA1, Chk1, and phospho-Cdc2/CDK1 protein expression. In vivo therapy studies showed 225-NP treatment reduced EGFR phosphorylation, increased γH2AX foci, and induced tumor cell apoptosis, resulting in suppression of tumor growth. Conclusion The 225-NP treatment induces DNA damage and abrogates G2/M phase of the cell cycle, leading to cellular apoptosis and suppression of lung tumor growth both in vitro and in vivo. Our findings provide a rationale for combining 225-NP with other DNA-damaging agents for achieving enhanced anticancer activity.

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Inhibition of nuclear factor-κB augments antitumor activity of adenovirus-mediated melanoma differentiation-associated gene-7 against lung cancer cells via mitogen-activated protein kinase kinase kinase 1 activation

Cited by 11 publications

References 39 publications

Fabrication of gold nanoparticles for targeted therapy in pancreatic cancer

Fabrication of gold nanoparticles for targeted therapy in pancreatic cancer

Mechanism underlying the inhibitory effect of Elaeagnus Conferta Roxb on TNF –α induced NF- kB nuclear translocation in non- small lung cancer A549 cell line using Flow cytometry

EGFR-targeted plasmonic magnetic nanoparticles suppress lung tumor growth by abrogating G2/M cell-cycle arrest and inducing DNA damage

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