Inhibition of Osteoclastic Bone Resorption by Calcitonin in the Cultured Medullary Bone of Laying Hens.

Sugiyama, Toshie; Ohashi, Tomoo; Kusuhara, Seiji

doi:10.2141/jpsa.30.16

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…This suggests that the adhesion activity of osteoclasts is dependent on cell activity, probably on the expression or the activation of receptors for substrate ligands. However, from the present study and other previous reports [16,18,[34][35][36], it is still uncertain whether the adhesion activity of the osteoclasts depends on the ligands or the receptors, or both. Therefore, more detailed studies of the ligands and receptors on the osteoclasts are needed using the present culture systems of medullary bone osteoclasts.…”

Section: Discussioncontrasting

confidence: 52%

“…As a result, these isolated osteoclasts lacked calcitonin receptors and did not response to calcitonin [1,9,17,24]. In contrast, osteoclasts isolated from the tibiae and femurs of chicks fed a calcium-and vitamin D-deficient diet and cultured in an organ culture system of medullary bone expressed calcitonin receptors, and the osteoclastic bone resorption was inhibited by calcitonin [5,6,27,30,34]. This discrepancy suggests that the hormonal responses of osteoclasts would be different according to the various in vivo conditions of the animals.…”

Section: Discussionmentioning

confidence: 97%

“…It has been reported that the osteoclastic bone resorption of medullary bone is stimulated by parathyroid hormone [35] and is inhibited by calcitonin [34]. Osteoclasts have recently been reported to express the mRNA of osteopontin [16,36] and the osteopontin mRNA expression is inhibited by calcitonin [18].…”

Section: Discussionmentioning

confidence: 99%

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Adhesive and Bone Resorptive Activities of Isolated Osteoclasts from Hen Medullary Bone.

Sugiyama

Kusuhara

1998

J. Vet. Med. Sci.

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ABSTRACT. In the present study, osteoclasts were isolated from hen medullary bones at the formative and resorptive phases. The cells were cultured on glass culture dishes and bone slices. After culturing, the adhesion activity of the isolated osteoclasts with the substrates was estimated with a light microscope, and the surfaces of the bone slices were observed with a scanning electron microscope. The results showed that the adhesion activity of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts is higher at the bone resorptive phase than at the bone formative phase, and this tendency in isolated osteoclasts was observed more frequently on the bone slices than on the glass culture dishes. Furthermore, scanning electron microscopy showed that the isolated osteoclasts in the bone resorptive phase adhered to the bone surface with developed-cytoplasmic projections and formed broad pits where collagen fibrils were exposed. On the other hand, isolated osteoclasts in the bone formative phase adhered to the bone slice with board-shaped cytoplasmic projections and did not form any pits. These results suggest that isolated osteoclasts in the bone resorptive phase have a high level of adhesion activity and actively resorb the bone, whereas isolated osteoclasts in the bone formative phase have a low level of adhesion activity and cease bone resorption. The procedure reported here is useful for studying the bone-resorptive mechanism of authentic osteoclasts. -KEY WORDS: adhesion activity, bone resorption, egg-laying cycle, medullary bone, osteoclast.J. Vet. Med. Sci. 60 (5): [573][574][575][576][577][578] 1998 doubtful that these isolated osteoclasts represent the authentic osteoclasts, and respond to the various calciumregulating factors in the same manner as authentic osteoclasts.In the present study, we attempted to isolate homogeneous active and inactive osteoclasts from hen medullary bones when the eggs are in the magnum and in the shell gland of the oviduct, respectively. We then evaluated the adhesion activity of these isolated osteoclasts on glass culture dishes and on bone slices, in order to clarify the role of bone matrix proteins in the osteoclast adhesion. In addition, the surface of the bone slices cultured with the isolated osteoclasts was observed with a scanning electron microscope. MATERIALS AND METHODS Animals:Laying White Leghorn hens (250 to 350 days of age) were given free access to tap water and a standard diet in an air conditioned (22 ± 2°C, room humidity 55 ± 5%) and a controlled light/dark room (15 hr/day of light). In the present study, three hens were used respectively at 3 hr (the bone formative phase) and 15 hr (the bone resorptive phase) after oviposition. The location of the egg in the oviduct was ascertained by autopsy, which revealed that three hens had eggs in the magnum of the oviduct (3 hr after oviposition), and the other three had eggs in the shell gland of the oviduct (15 hr).Isolation and culturing of medullary bone osteoclasts: The procedure for the culture of medull...

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 97%

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Adhesive and Bone Resorptive Activities of Isolated Osteoclasts from Hen Medullary Bone.

Sugiyama

Kusuhara

1998

J. Vet. Med. Sci.

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“…The mechanism that is the basis of this effect should be the inhibition of succinate dehydrogenase (SDH, complex II) 30 in agreement with the hypothesis that cells destined to mineralize rely less on respiration. (31) However, the inhibition of Krebs enzymes in actively resorbing osteoclast could reduce their activity, as indirectly indicated by the stimulatory or inhibitory effects on osteoblastic SDH exerted by PTH (32) or calcitonin, (33) respectively.…”

Section: Discussionmentioning

confidence: 99%

Tartronates: A New Generation of Drugs Affecting Bone Metabolism

Caselli

Mantovanini

Gandolfi

et al. 1997

Journal of Bone and Mineral Research

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In the search for a new class of bone-sparing agents for treating osteopenic disorders, we hypothesized that tartronic acid derivatives, sharing the chemical characteristics both of bisphosphonates and of Gla residues contained in matrix proteins such as osteocalcin, could positively affect bone metabolism. A series of tartronates was therefore tested for their ability to affect bone metabolism. In vitro resorption tests were performed examining pit formation by freshly isolated rat and rabbit osteoclasts plated onto bone slices and exposed to the drugs for 48 h. Tartronates bearing a linear side-chain (DF 1222 and DF 1363A) were the most effective in inhibiting pit excavation in the pM-nM range. Tartronates did not affect osteoclast viability, number, adhesion, or tartrate resistant acid phosphatase activity. Transient cell retraction was observed in osteoclasts plated onto glass and exposed to DF 1222. The maximal effect was seen in cells treated for 4 h at a concentration of 1 pM. DF 1222 accelerated mineralization in cultures of periosteal cells without affecting other osteoblast-like functions. This product was therefore tested in vivo in ovariectomized mice. Bone mass in femur was evaluated, by ash gravimetry, 21 days after ovariectomy. Unfortunately, DF 1222, the most active of tartronates in vitro, was inactive in this test because of its high hydrophilicity and the subsequent too short residence time. On the contrary, its tetrahydropyranyl ether derivative, DF 1363A, endowed with a significantly higher lipophilicity, showed a dose-dependent bone-sparing effect when administered subcutaneously at 10, 30, and 100 mg/kg/die, thus confirming the activity seen in in vitro tests. Because of their feasible parallel effect on both bone resorption and formation, tartronate derivatives may be tested to candidate this class of products for clinical studies. (J Bone Miner Res 1997;12:972-981)

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The Role of Hormones in the Regulation of Bone Turnover and Eggshell Calcification

Dacke

Sugiyama

2015

Sturkie's Avian Physiology

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Inhibition of Osteoclastic Bone Resorption by Calcitonin in the Cultured Medullary Bone of Laying Hens.

Cited by 6 publications

References 26 publications

Adhesive and Bone Resorptive Activities of Isolated Osteoclasts from Hen Medullary Bone.

Adhesive and Bone Resorptive Activities of Isolated Osteoclasts from Hen Medullary Bone.

Tartronates: A New Generation of Drugs Affecting Bone Metabolism

The Role of Hormones in the Regulation of Bone Turnover and Eggshell Calcification

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