Inhibition of ovarian microsomal aromatase and follicular oestradiol secretion by imidazole fungicides in Rainbow trout

Monod, Gilles; Mones, A. de; Fostier, Alexis

doi:10.1016/0141-1136(93)90030-4

Cited by 72 publications

(34 citation statements)

References 11 publications

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“…Clotrimazole also inhibits other cytochrome P450s, and concern has been expressed that it has the potential to elicit endocrinedisrupting effects in exposed wildlife or humans. Specifically, imidazole antifungal agents inhibit cytochrome P450 aromatases, catalyzing the conversion of steroidal androgens to estrogens in rainbow trout (Oncorhynchus mykiss; [11]), frogs (Xenopus tropicalis; [12]), and humans [13][14][15], promote testosterone accumulation in human volunteers [16], and promote testicular development in female salmon (Oncorhynchus tshawytscha [17]). …”

Section: Introductionmentioning

confidence: 99%

Fate of the antifungal drug clotrimazole in agricultural soil

Sabourin

Al-Rajab

Chapman

et al. 2010

Enviro Toxic and Chemistry

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Clotrimazole is a broad-spectrum antimycotic drug used for the treatment of dermatological and gynecological infections; it is incompletely broken down during sewage treatment and could potentially reach agricultural land through the application of municipal biosolids or wastewater. In the absence of any environmental fate data, we evaluated the persistence and dissipation pathways of (3)H-clotrimazole during laboratory incubations of agricultural soils. Clotrimazole was removed from a loam (time to dissipate 50% = 68 d), a sandy loam (time to dissipate 50% = 36 d), and a clay loam (time to dissipate 50% = 55 d), with formation of nonextractable residues being the major sink for (3) H. Their parent compound had no significant mineralization, as evidenced by the lack of formation of (3) H(2) O. Up to 15% of the applied radioactivity was recovered in the form of [(3)H]-(2-chlorophenyl)diphenyl methanol. The rate of clotrimazole dissipation in the loam soil did not vary with moisture content, but it was slower at a lower temperature (number of days to dissipate 50% = 275.6 d at 4°C). Addition of municipal biosolids to the loam soil did not vary the clotrimazole dissipation rate. In summary, the present study has established that clotrimazole is dissipated in soil, at rates that varied with soil texture and temperature. Clotrimazole dissipation was accompanied by the formation of nonextractable residues and detectable extractable residues of the transformation product (2-chlorophenyl)diphenyl methanol.

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Section: Introductionmentioning

confidence: 99%

Fate of the antifungal drug clotrimazole in agricultural soil

Sabourin

Al-Rajab

Chapman

et al. 2010

Enviro Toxic and Chemistry

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“…Moreover, these chemicals could lead to the inhibition of cytochrome (CYP) P-450 dependent C14 demethylation of lanosterol, an intermediate in ergosterol biosynthesis [2]. In fish, CYP mediated steroid metabolism [5] and xenobiotic metabolism [6] can be altered.…”

Section: -[ [2-(24-dichlorophenyl)-4-propyl-13-dioxolan-2-yl]mentioning

confidence: 99%

Biochemical responses in gills of rainbow trout exposed to propiconazole

Li¹,

Žlábek

Grabic

et al. 2010

Open Life Sciences

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The aim of this study is to investigate the toxic effect of PCZ, a triazole fungicide commonly present in surface and ground water, on the ROS defense system and Na+-K+-ATPase in gills of rainbow trout exposed to sublethal concentrations (0.2, 50 and 500 μg L−1) for 7, 20 and 30 days. After prolonged exposure of PCZ at higher test concentrations (50 and 500 μg L−1), oxidative stress was apparent as reflected by the significant higher ROS levels in fish gill, as well as the significant inhibition of SOD and CAT activities. In addition, Na+-K+-ATPase activities were significantly lower than those of the control with increasing PCZ concentration and prolonged exposure period. The results of this study indicate that chronic exposure to PCZ has altered multiple physiological indices in fish gill; however, before these parameters are used as unique biomarkers for monitoring residual pharmaceuticals in aquatic environments, more detailed laboratory experiments need to be performed.

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“…The tritiated water release assay for aromatase activity determination was adapted from previous studies (Gore-Langton & Dorrington 1981, Monod et al 1993) using 450 ml Xenopus extracts in duplicates adjusted to 500 ml after adding the cofactor NADPH (1 mM, Sigma-Aldrich) and 1b 3 H-androstenedione (s.a.Z0 . 87 TBq/mmol, PerkinElmer, Boston, MA, USA; 19 kBq to a final concentration of 50 nM).…”

Section: Aromatase Activity Assaymentioning

confidence: 99%

Aromatase expression in Xenopus oocytes: a three cell-type model for the ovarian estradiol synthesis

Gohin¹,

Bodinier²,

Fostier³

et al. 2011

Journal of Molecular Endocrinology

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In contrast to the classical model describing the synthesis of androgens and estrogens as restricted to somatic cells, a previous study demonstrated that Xenopus laevis oocytes participate in androgen synthesis. The objective of our study was to determine whether Xenopus oocytes are also involved in estrogen synthesis. More precisely, we analyzed aromatase expression by in situ hybridization and RT-QPCR and measured aromatase activity. Aromatase, the enzyme responsible for estrogen synthesis, appears to be expressed and active not only in the follicular cells but also in the vitellogenic oocytes. During late oogenesis, aromatase oocyte expression and activity decreased concomitantly with the trend observed in surrounding follicular layers. In order to investigate the role of estradiol-17b (E 2 ), we studied its effect on oocyte meiotic resumption. It appears that, as in Rana pipiens, E 2 inhibited the follicle-enclosed maturation of Xenopus oocytes, likely through inhibition of LH-induced maturation-inducing steroid synthesis. In addition, E 2 exerted a slight enhancing action on denuded oocyte maturation whose biological significance remains unclear. Together, our results demonstrate that Xenopus oocyte significantly participates in ovarian E 2 synthesis and this may be a common feature of vitellogenic vertebrates.

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Inhibition of ovarian microsomal aromatase and follicular oestradiol secretion by imidazole fungicides in Rainbow trout

Cited by 72 publications

References 11 publications

Fate of the antifungal drug clotrimazole in agricultural soil

Fate of the antifungal drug clotrimazole in agricultural soil

Biochemical responses in gills of rainbow trout exposed to propiconazole

Aromatase expression in Xenopus oocytes: a three cell-type model for the ovarian estradiol synthesis

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