Inhibition of PMN apoptosis after adherence to dip-coated calcium phosphate surfaces on a NiTi shape memory alloy

Bogdanski, D.; Esenwein, S. A.; Prymak, Oleg; Epple, Matthias; Muhr, G.; Köller, Manfred

doi:10.1016/j.biomaterials.2003.12.001

Cited by 39 publications

(39 citation statements)

References 19 publications

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“…Furthermore, it is interesting that the detached cells do not undergo apoptosis after detaching. This may indicate that the detachment is occurring during or media exchange, or possibly that the interaction of the cells with the PA is leading to the release of soluble mediators that cause the inhibition of apoptosis [56]. Third, the results confirm that there is no cytotoxic effect from any residual reagents used for covalent attachment.…”

Section: Cellular Response To Pa Coated Niti Samplessupporting

confidence: 71%

Covalent functionalization of NiTi surfaces with bioactive peptide amphiphile nanofibers

et al. 2008

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Surface modification enables the creation of bioactive implants using traditional material substrates without altering the mechanical properties of the bulk material. For applications such as bone plates and stents, it is desirable to modify the surface of metal alloy substrates to facilitate cellular attachment, proliferation, and possibly differentiation. In this work we present a general strategy for altering the surface chemistry of nickel-titanium shape memory alloy (NiTi) in order to covalently attach self-assembled peptide amphiphile (PA) nanofibers with bioactive functions. Bioactivity in the systems studied here includes biological adhesion and proliferation of osteoblast and endothelial cell types. The optimized surface treatment creates a uniform TiO 2 layer with low levels of Ni on the NiTi surface, which is subsequently covered with an aminopropylsilane coating using a novel, lower temperature vapor deposition method. This method produces an aminated surface suitable for covalent attachment of PA molecules containing terminal carboxylic acid groups. The functionalized NiTi surfaces have been characterized by X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectroscopy (ToF-SIMS), and atomic force microscopy (AFM). These techniques offer evidence that the treated metal surfaces consist primarily of TiO 2 with very little Ni, and also confirm the presence of the aminopropylsilane overlayer. Self-assembled PA nanofibers presenting the biological peptide adhesion sequence Arg-Gly-Asp-Ser are capable of covalently anchoring to the treated substrate, as demonstrated by spectrofluorimetry and AFM. Cell culture and scanning electron microscopy (SEM) demonstrate cellular adhesion, spreading, and proliferation on these functionalized metal surfaces. Furthermore, these experiments demonstrate that covalent attachment is crucial for creating robust PA nanofiber coatings, leading to confluent cell monolayers.

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Section: Cellular Response To Pa Coated Niti Samplessupporting

confidence: 71%

Covalent functionalization of NiTi surfaces with bioactive peptide amphiphile nanofibers

et al. 2008

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“…21 Also, cytokine release positively correlates with material surface roughness. 9,[22][23][24] These observations are in concordance with the results of our present study, which revealed the most pronounced cytokine release with tantalum TM, being the material with the roughest surface and highest P a -value. Also the whole blood killing was significantly increased by CM obtained from the roughest test samples (TM, Ta).…”

Section: Discussionsupporting

confidence: 96%

Activation of human leukocytes on tantalum trabecular metal in comparison to commonly used orthopedic metal implant materials

Schildhauer

Peter

Muhr

et al. 2008

J Biomedical Materials Res

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We analyzed leukocyte functions and cytokine response of human leukocytes toward porous tantalum foam biomaterial (Trabecular Metaltrade mark, TM) in comparison to equally sized solid orthopedic metal implant materials (pure titanium, titanium alloy, stainless steel, pure tantalum, and tantalum coated stainless steel). Isolated peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) were cocultured with equally sized metallic test discs for 24 h. Supernatants were analyzed for cytokine content by enzyme-linked immunosorbent assay. Compared to the other used test materials there was a significant increase in the release of IL (interleukin)-1ra and IL-8 from PMN, and of IL-1ra, IL-6, and TNF-alpha from PBMC in response to the TM material. The cytokine release correlated with surface roughness of the materials. In contrast, the release of IL-2 was not induced showing that mainly myeloid leukocytes were activated. In addition, supernatants of these leukocyte/material interaction (conditioned media, CM) were subjected to whole blood cell function assays (phagocytosis, chemotaxis, bacterial killing). There was a significant increase in the phagocytotic capacity of leukocytes in the presence of TM-conditioned media. The chemotactic response of leukocytes toward TM-conditioned media was significantly higher compared to CM obtained from other test materials. Furthermore, the bactericidal capacity of whole blood was enhanced in the presence of TM-conditioned media. These results indicate that leukocyte activation at the surface of TM material induces a microenvironment, which may enhance local host defense mechanisms.

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“…Among the leukocytes polymorphonuclear neutrophil granulocytes (PMN) were identified as the predominant adherent cell population [3]. Beside an increased number of adherent PMN we observed that the apoptosis of adherent PMN was significantly delayed on CaPcoated NiTi-samples and also in the close vicinity of the CaPcoated samples [6]. In addition, when peripheral blood mononuclear cells (PBMC) were incubated together with CaPcoated NiTi-samples, an aggregation of PBMC was not only observed on the surfaces of CaP-coated NiTi-samples but also in the vicinity of the CaP-coated samples.…”

Section: Introductionmentioning

confidence: 94%

Regulation of leukocyte adhesion molecules by leukocyte/biomaterial‐conditioned media: A study with calcium‐phosphate‐coated and non‐coated NiTi‐shape memory alloys

Köller¹,

Esenwein

Bogdanski

et al. 2006

Materialwissenschaft Werkst

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To evaluate the role of soluble mediators released after interaction of leukocytes with biomaterials on cells of the implant microenvironment, the surface expression of adhesion molecules (CD11b, CD62L, CD66b, ICAM-1) on freshly isolated whole blood leukocytes (WBL) was analyzed by flow cytometry after incubation in leukocyte/nickel-titanium shape-memory alloy (NiTi-SMA)-conditioned media. NiTi-SMA samples were either coated with calcium phosphate by dipping in oversaturated calcium phosphate solution (CaP-coating) or were non-coated. Peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) were isolated and seeded on both samples to generate respective conditioned media. In comparison with conditioned media obtained by non-coated NiTi-SMA, the expression of CD11b, CD66b, and ICAM-1 on WBL was upregulated by conditioned media obtained from CaP-coated samples. In contrast, the expression of CD62L on WBL was decreased after incubation in conditioned medium obtained from CaP-coated NiTi-samples compared to noncoated NiTi. These data demonstrate the possible influence of released mediators elicited by leukocyte-biomaterial interactions on cells of the implant microenvironment.

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Inhibition of PMN apoptosis after adherence to dip-coated calcium phosphate surfaces on a NiTi shape memory alloy

Cited by 39 publications

References 19 publications

Covalent functionalization of NiTi surfaces with bioactive peptide amphiphile nanofibers

Covalent functionalization of NiTi surfaces with bioactive peptide amphiphile nanofibers

Activation of human leukocytes on tantalum trabecular metal in comparison to commonly used orthopedic metal implant materials

Regulation of leukocyte adhesion molecules by leukocyte/biomaterial‐conditioned media: A study with calcium‐phosphate‐coated and non‐coated NiTi‐shape memory alloys

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