Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY

Herpin, Amaury; Schindler, Detlev; Kraiss, Anita; Hornung, Ute; Winkler, Christoph; Schartl, Manfred

doi:10.1186/1471-213x-7-99

Cited by 61 publications

(55 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Gene knockdown by injecting gripNA against dmrt1bY into one-cell stage XY embryos also shows an increased number of germ cells compared to normal XY larvae and entrance into meiosis at 0 days after hatching [Paul-Prasanth et al, 2006]. In agreement, dmrt1bY -morpholino injection did not affect genotypic females but affected a fraction of the males, causing primordial gonad phenotypes that ranged from complete to partial male-to-female sex reversion, observed in the number of PGCs [Herpin et al, 2007b].…”

Section: The Function and Role Of Dmrt1by In Determining Male Developsupporting

confidence: 54%

“…Cell proliferation is inhibited in embryos overexpressing dmrt1bY , and cells are arrested in the G2 phase of the cell cycle [Herpin et al, 2007b]. It is highly likely that dmrt1bY is responsible for male germ cell arrest, thus the proliferation of germ cells in males begins much later than in females, starting later than 15 days after hatching.…”

Section: The Function and Role Of Dmrt1by In Determining Male Developmentioning

confidence: 99%

“…From these studies, 2 major roles of dmrt1bY in sex determination or differentiation could be postulated. In addition, the Dmrt1bY protein seems to work in 2 modes, either cell autonomously or in a paracrine manner [Herpin et al, 2007b].…”

Section: The Function and Role Of Dmrt1by In Determining Male Developmentioning

confidence: 99%

See 2 more Smart Citations

Sex Determination and Sex Chromosome Evolution: Insights from Medaka

Kondo

Nanda²,

Schmid³

et al. 2009

Sex Dev

View full text Add to dashboard Cite

Among fish that exhibit a variety of sex-determining systems, medaka (Oryzias latipes) has been used as an ideal model to study sex determination and differentiation. Medaka sex differentiation starts with germ cell migration and proliferation during development, and the earliest sexual difference can be seen in germ cell number at stage 38 (1 day before hatching). Differentiation continues in young larvae. Medaka has an XX-XY genetic system, and the male sex-determining gene dmrt1bY (dmy) has been identified. Along with a sister species O. curvinotus, these species are the only 2 fish species whose sex-determining gene has been identified so far. The medaka sex chromosome is of recent origin, and the Y chromosome was formed through generation of the Y-specific region by duplication and insertion of an autosomal region into the proto-Y chromosome. Thus, the dmrt1bY gene is a duplicate of the autosomal dmrt1a gene. This event is estimated to have occurred about 10 million years ago. Sex-determining systems of other Oryzias species are under investigation, and a variety of sex-determining systems seem to exist. These findings may provide new insights into the sex-determining system of medaka.

show abstract

Section: The Function and Role Of Dmrt1by In Determining Male Developsupporting

confidence: 54%

Section: The Function and Role Of Dmrt1by In Determining Male Developmentioning

confidence: 99%

See 1 more Smart Citation

Sex Determination and Sex Chromosome Evolution: Insights from Medaka

Kondo

Nanda²,

Schmid³

et al. 2009

Sex Dev

View full text Add to dashboard Cite

show abstract

“…Herpin et al [72] observed that DMY could arrest germ cells of male medaka in the G2 phase. In situ hybridization in medaka showed that mis mRNA was expressed in the somatic cells surrounding germ cells during sex differentiation, and that MIS protein was thought to promote germ cells differentiation by acting on the somatic cells [73].…”

Section: Development Of Fish Germ Cellsmentioning

confidence: 99%

Progress in studies of fish reproductive development regulation

Zhu

2012

Chin. Sci. Bull.

View full text Add to dashboard Cite

Mechanisms of the animal reproductive development are an important research field in life sciences. The study of the reproductive development and regulatory mechanisms in fishes is important for elucidating the mechanisms of animal reproduction. This paper summarizes recent advances in the mechanisms of fish sex determination and differentiation, of fish gonad development and maturation, and of fish germ cell development, as well as the according regulating strategies. Fishes comprise an evolutionary stage that links invertebrates and higher vertebrates. They include diversiform species, and almost all vertebrate types of reproduction have been found in fishes. All these will lead to important advances in the regulatory mechanisms of animal reproduction by using fishes as model organisms. It will also enable novel fish breeding techniques when new controllable on-off strategies of reproduction and/or sex in fishes have been developed. fish, reproduction, regulation Citation:Chen J, Hu W, Zhu Z Y. Progress in studies of fish reproductive development regulation. Chin Sci Bull, 2013, 58: 716, doi: 10.1007/s11434-012-5577-1As low vertebrates, fishes are on an evolutionary stage linking invertebrates and higher vertebrates. There are as many as 28000 known fish species, which show large diversity. A range of sexual characteristics and reproductive strategies has been described for fishes, and almost all reproductive types of vertebrates have been found in fishes. The study of fish reproductive development regulation is important for elucidating mechanisms of animal reproductive development. Molecular breeding will reveal finer varieties when genes and regulatory networks associated with economically important traits (such as fast growth, disease resistance, cold tolerance and hypoxia tolerance) in fishes are discovered [1]. Reproduction also provides a precondition for culturing exclusive fish breeds and expanding fish populations. Breeding techniques such as sexual control and fertility control have played important roles in developing new fish varieties. This paper will review advances of fish reproductive development regulation, with the aim of providing hints for mechanisms of fish reproductive development, as well as providing theoretical guidance for techniques of reproductive regulation and developing highyielding, efficient, eco-friendly breeds.1 Sexual control of fishes Factors influencing sex determinationThe sex differentiation pattern and determination mechanism of fishes are very diverse. Various sexual phenotypes are all present in fishes, such as hermaphroditism, unisexuality, gonochorism and dual reproduction mode including parthenogenesis and sexual reproduction [2]. For those fishes in which sex chromosome systems exist, there are single chromosome systems, such as XX/XY, ZZ/ZW, XX/ XO and ZZ/ZO, and multiple chromosome systems, such as XX/XY 1 Y 2 , ZZ/ZW 1 W 2 , X 1 X 2 X 1 X 2 /X 1 X 2 Y, and X 1 X 2 X 1 X 2 / X 1 X 2 X 1 . Like other animals, fish sex differentiation is controlled by their gene...

show abstract

“…For microscopic examination embryos were transferred into icecold PBS, 0.01% 3-aminobenzoic acid ethyl ester methane sulfonate. Terminal transferase-mediated dUTP nick end-labeling (TUNEL) assays were performed as described elsewhere (20).…”

Section: Methodsmentioning

confidence: 99%

lin9 Is Required for Mitosis and Cell Survival during Early Zebrafish Development

Kleinschmidt

Wagner

Liedtke

et al. 2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

LIN9 has been described as a regulator of G 1 /S and G 2 /M progression of the cell cycle in invertebrates and human cell lines. To elucidate the in vivo function of LIN9 during vertebrate development, we took advantage of the teleost zebrafish (Danio rerio). By means of antisense morpholinos we show here that Lin9-depleted embryonic cells accumulate in mitosis. Flow cytometry and confocal microscopy data demonstrate that the delay in mitotic progression is followed by apoptosis, which strongly manifests in the developing central nervous system. In accordance with these findings, we identified a cohort of Lin9-regulated genes required for different mitotic processes, including mitotic entry, metaphase/anaphase transition, and cytokinesis. Our data establish LIN9 as an essential regulator of mitosis in vertebrate development.The transition from G 1 to S phase marks the starting point of the eukaryotic cell to replicate its chromosomal set of DNA. This event is followed by the G 2 period that prepares the cell for mitosis, a process that results in the separation of the duplicate chromosome set into two nuclei. The progression through the cell cycle is regulated by multiple processes, including regulated gene transcription. For example, E2F transcription factors play a key role in the activation of S phase entry genes during the G 1 phase of the cell cycle (reviewed in Ref. 1). Likewise, several transcription factors are implicated to play a role in the activation of mitotic genes during S phase, among them NF-Y, B-MYB, and FOXM1 (2-4).LINC/DREAM is a recently identified multiprotein complex that is required for two transcriptional processes that act on cell cycle regulation, namely repression of genes that drive G 1 /S transition and activation of genes required for G 2 /M progression (3,5,6). LINC/DREAM consists of the core members LIN9, LIN54, LIN37, LIN52, and RBAP48. Interestingly, LINC/ DREAM undergoes a dynamic and cell cycle-dependent switch of subunits (7,8). In G 0 /G 1 , the complex is associated with p130 and E2F4, whereas in late S phase it interacts with B-MYB and activates G 2 /M promoters. LINC/DREAM is evolutionarily highly conserved. A DREAMlike complex was first purified from Drosophila embryo lysates and was named Myb-MuvB (MMB) or dREAM (for Drosophila RBF-, dE2F2-, and dMyb-interacting proteins) (9, 10). Although initially described as complexes involved in the repression of E2F target genes during fly development, a recent study indicates that dREAM/Myb-MuvB also regulate the activation of genes required for mitotic progression (11). Furthermore, a related complex has also been identified in Caenorhabditis elegans (12).B-MYB, a subunit of the activating LINC complex in S phase and G 2 , is essential for early mouse embryogenesis, and its short hairpin RNA-mediated knockdown in murine ES cells results in a delay of G 2 /M progression, mitotic spindle and centrosome defects, and polyploidy (13,14). In zebrafish, the bmyb loss-offunction mutation crash&burn (crb) results in a similar phenotype....

show abstract

Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY

Cited by 61 publications

References 45 publications

Sex Determination and Sex Chromosome Evolution: Insights from Medaka

Sex Determination and Sex Chromosome Evolution: Insights from Medaka

Progress in studies of fish reproductive development regulation

lin9 Is Required for Mitosis and Cell Survival during Early Zebrafish Development

Contact Info

Product

Resources

About