Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer

Zhu, Xiaogang; Gao, Guanghui; Chu, Kaili; Yang, Xiufang; Ren, Shengxiang; Yao, Li; Wu, Hai; Huang, Yan; Zhou, Caicun

doi:10.1016/j.biocel.2015.02.005

Cited by 40 publications

(28 citation statements)

References 36 publications

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“…A previous study showed that retinoic acid has been reported to have the effect on the inhibition of lung cancer cell. It could increase the expression of miR-512-3p in microRNA, since overexpression of miR-512-3p could not only inhibit tumour cell adhesion, migration and invasion in NSCLC A549 and H1299 cell lines, but could also decrease the DOCK3 protein (44) . Furthermore, some types of carotenoids are converted into vitamin A, which is related to cellular differentiation may contribute to cancer prevention (45) .…”

Section: Discussionmentioning

confidence: 99%

Is carrot consumption associated with a decreased risk of lung cancer? A meta-analysis of observational studies

Jiang

Yang

et al. 2019

Br J Nutr

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Findings of epidemiological studies regarding the association between carrot consumption and lung cancer risk remain inconsistent. The present study aimed to summarise the current epidemiological evidence concerning carrot intake and lung cancer risk with a meta-analysis. We conducted a meta-analysis of case–control and prospective cohort studies, and searched PubMed and Embase databases from their inception to April 2018 without restriction by language. We also reviewed reference lists from included articles. Prospective cohort or case–control studies reporting OR or relative risk with the corresponding 95 % CI of the risk lung cancer for the highest compared with the lowest category of carrot intake. A total of eighteen eligible studies (seventeen case–control studies and one prospective cohort study) were included, involving 202 969 individuals and 5517 patients with lung cancer. The pooled OR of eighteen studies for lung cancer was 0·58 (95 % CI 0·45, 0·74) by comparing the highest category with the lowest category of carrot consumption. Based on subgroup analyses for the types of lung cancer, we pooled that squamous cell carcinoma (OR 0·52, 95 % CI 0·19, 1·45), small-cell carcinoma (OR 0·43, 95 % CI 0·12, 1·59), adenocarcinoma (OR 0·34, 95 % CI 0·15, 0·79), large-cell carcinoma (OR 0·40, 95 % CI 0·10, 1·57), squamous and small-cell carcinoma (OR 0·85, 95 % CI 0·45, 1·62), adenocarcinoma and large-cell carcinoma (OR 0·20, 95 % CI 0·02, 1·70) and mixed types (OR 0·61, 95 % CI 0·46, 0·81). Exclusion of any single study did not materially alter the pooled OR. Integrated epidemiological evidence from observational studies supported the hypothesis that carrot consumption may decrease the risk of lung cancer, especially for adenocarcinoma.

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Section: Discussionmentioning

confidence: 99%

Is carrot consumption associated with a decreased risk of lung cancer? A meta-analysis of observational studies

Jiang

Yang

et al. 2019

Br J Nutr

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“…The present study revealed that miR-512-3p was significantly upregulated in PCa compared with in normal tissues; however, to the best of our knowledge, the molecular functions of miR-512-3p in PCa have yet to be reported in PCa. In lung adenocarcinoma, miR-512-3p has been reported to act as a tumor suppressor that inhibits cell adhesion, migration and invasion of NSCLC cells (23). However, the roles of miR-512-3p in PCa remain unclear.…”

Section: Discussionmentioning

confidence: 99%

“…miR-512-3p has been reported to act as a tumor suppressor in hepatocellular carcinoma (22) and lung adenocarcinoma (23). In addition, miR-512-3p has also been revealed to be upregulated in non-small cell lung cancer (NSCLC) A549 cells following retinoic acid (RA) treatment, and has been demonstrated to inhibit the adhesion, migration and invasion of NSCLC cells (23). However, the role of miR-512-3p in PCa remains poorly understood and therefore requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑512‑3p is upregulated, and promotes proliferation and cell cycle progression, in prostate cancer cells

Rao

et al. 2017

Mol Med Report

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Prostate cancer (PCa) is the most commonly diagnosed cancer in males worldwide. MicroRNAs (miRNAs/miRs) are small non‑coding RNAs that participate in the regulation of various biological processes by regulating post‑transcriptional gene expression. However, whether dysregulation of miRNA expression may be associated with the carcinogenesis of PCa remains to be elucidated. The present study identified differentially expressed miRNAs in PCa by analyzing two publicly available gene expression datasets, GSE14857 and GSE21036. The results demonstrated that miR‑512‑3p was significantly upregulated in PCa. Furthermore, the present study explored the molecular functions of miR‑512‑3p in PCa, and demonstrated that overexpression of miR‑512‑3p promoted PCa cell proliferation and reduced G1 phase cell cycle arrest in PCa. These results indicated that miR‑512‑3p may act as an oncogene in PCa. To the best of our knowledge, this is the first study revealed the molecular functions of miR‑512‑3p in PCa. To obtain valuable insights into the potential mechanisms of miR‑512‑3p, bioinformatics analyses were performed to identify the targets of miR‑512‑3p. Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology category analyses revealed that miR‑512‑3p may be associated with the mitogen‑activated protein kinase signaling pathway and numerous biological processes, including cell adhesion, cell proliferation, cell cycle and apoptosis. These results suggested that miR‑512‑3p may be considered a potential diagnostic and therapeutic target of PCa.

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“…Over the past few years, microRNAs (miRNAs/miRs) have been demonstrated to be involved in the pathogenesis of human lung cancer (8). miRNAs, a class of small non-coding RNAs (~22 nucleotides long), are widely expressed in eukaryotes and regulate the expression of their target mRNAs by base pairing with mRNAs in the 3'-untranslated region (UTR), leading to mRNA cleavage or translation repression (9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1

Liu

Xia

et al. 2016

Oncology Letters

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Abstract. The expression of microRNA-181b (miR-181b) has been investigated in various human cancers. However, the expression and functions of miR-181b in non-small cell lung cancer (NSCLC) are yet to be studied. In the present study, miR-181b expression in NSCLC tissues and cell lines was analyzed by quantitative polymerase chain reaction, and was shown to be recurrently downregulated. Following transfection of the H23 and H522 NSCLC cells lines with miR-181b, cell migration and cell invasion assays were performed to evaluate the effect of miR-181b overexpression on the cell motility. It was demonstrated that overexpression of miR-181b inhibited the migration and invasion of NSCLC cells. Subsequently, bioinformatics analysis, western blotting and luciferase reporter assays were conducted to investigate the mechanism underlying the miR-181b-mediated inhibition of NSCLC cell motility. It was found that miR-181b directly targeted high-mobility group box-1 (HMGB1) in NSCLC cells. These results reveal a novel therapeutic target, the miR-181b/HMGB1 axis, in NSCLC. Treatment approaches targeting this axis will be beneficial to prevent NSCLC from becoming invasive.

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Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer

Cited by 40 publications

References 36 publications

Is carrot consumption associated with a decreased risk of lung cancer? A meta-analysis of observational studies

Is carrot consumption associated with a decreased risk of lung cancer? A meta-analysis of observational studies

MicroRNA‑512‑3p is upregulated, and promotes proliferation and cell cycle progression, in prostate cancer cells

MicroRNA-181b is downregulated in non-small cell lung cancer and inhibits cell motility by directly targeting HMGB1

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