Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds

Blöchl, Andreas; Peterbauer, Thomas; Richter, Andreas

doi:10.1016/j.jplph.2006.10.010

Cited by 93 publications

(87 citation statements)

References 18 publications

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“…Current pea cultivars are characterized by a higher level of RFO in dry matter (46.8 vs 80.6 g · kg -1 DM), with stachyose and verbascose exhibiting the highest increase compared with the results obtained by Zduńczyk et al (1997). According to some researchers, seed germination capacity depends considerably on oligosaccharide levels (Blöchl et al, 2007). The observed significant increase in oligosaccharide content in pea cultivars can be attributed to genetic improvements aimed at enhancing seed germination capacity.…”

Section: Discussionmentioning

confidence: 99%

Factors affecting the nutritional value of pea (<i>Pisum sativum</i>) for broilers

2015

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Section: Discussionmentioning

confidence: 99%

Factors affecting the nutritional value of pea (<i>Pisum sativum</i>) for broilers

2015

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“…At the initial stages of seed germination and seedling development, both glucose and sucrose can be derived from other sources, than lipids or polysaccharides. In nonendospermic seeds, like legumes, during the initial stages of seed germination sucrose and raffinose family oligosaccharides (RFOs) play a role of primary sources of carbon skeletons and energy for tissues of growing seedling (Blöchl et al 2007). The concentration of RFOs dramatically decreases in axis during the first 2 days of seed germination, and breakdown of RFOs is continued for next few days in cotyledons (Lahuta and Górecki 2011;Lahuta et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Application of phytohormones during seed hydropriming and heat shock treatment on sunflower (Helianthus annuus L.) chilling resistance and changes in soluble carbohydrates

Górnik

Lahuta

2017

Acta Physiol Plant

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The objective of this study was to analyze the mechanism of some physiological processes accompanying acquisition of sunflower (Helianthus annuus L.) chilling resistance due to seeds hydropriming in the presence of salicylic acid, jasmonic acid, 24-epibrassinolide followed exposition of seeds to short-term heat shock treatment. The seeds were hydroprimed at 25°C in limited amounts of water or solution of salicylic or jasmonic acid at 10 -2 , 10and 10 -4 M concentration, 24-epibrassinolide at 10 -6 , 10 -8 and 10 -10 M concentration. The seeds were incubated for 2 days, subjected to short-term heat shock (45°C, 2 h) and chilled for 21 days at 0°C. Sunflower chilling susceptibility and physiological responses were evaluated according to the inhibition of radicle growth, the inhibition of the number of lateral roots formation, the activity of catalase and changes in soluble carbohydrates in seedlings developing for 72 h at 25°C. Hydropriming and short-term heat shock application explicitly reduced inhibition of roots as well as lateral roots development by allowing the germinating seeds to recover from the growthinhibiting effects of chilling. Seeds hydropriming in solutions containing salicylic acid, jasmonic acid and 24-epibrassinolide followed heat shock treatment additionally promoted the activity of catalase and sugars metabolism, which stimulated seedlings development and alleviated the decrease of F v /F m caused by chilling conditions. These beneficial effects contributed to increased resistance of sunflower seedlings to chilling stress. The present study demonstrated that the most profitable effect on reducing negative effect of chilling may be achieved by short-term heat shock applied during hydropriming in water supplemented with 24-epiBL (10 -8 and 10 -10 M) or salicylic acid (10 -3 and 10 -4 M).

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“…RFOs are typical seed storage compounds which are quickly hydrolyzed during initial germination steps. Inhibition of RFO degradation delayed germination of seeds of pea (Blöchl et al, 2007), winter vetch (Lahuta and *e-mail: lahuta@uwm.edu Goszczyńska, 2009) and wild type soybean but not soybean lines with decreased levels of RFOs (Dierking and Bilyeu, 2009). Overexpression of α-galactosidase in pea seeds leads to 40% reduction of RFOs without any effect on seed viability and germination (Polowick et al, 2009;Obendorf et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Seed α-D-Galactosides of Selected Vicia Species and Enzymes Involved in Their Biosynthesis

Lahuta¹,

Goszczyńska²,

Horbowicz³

2010

Acta Biologica Cracoviensia Series Botanica

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We compared the soluble carbohydrate composition of seeds of ten wild and cultivated species of the genus Vicia. In some Vicia species (V. angustifolia, V. grandiflora, V. sativa, V. sepium) they contained only raffinose family oligosaccharides (RFOs) and in others also D-pinitol and its α-D-galactosides. In terms of galactosyl pinitol composition they were divided into three groups: those accumulating small amounts of mono-, di-, tri-galactosyl pinitol A (GPA, ciceritol and TGPA, respectively) and unknown compound (V. sylvatica and V. hirsuta); those accumulating more ciceritol than TGPA (V. tetrasperma and V. villosa); and those accumulating more TGPA than ciceritol (V. cracca and V. tenuifolia). The differences in the activity of galactosyltransferases engaged in RFOs and galactosyl pinitol synthesis confirmed this classification. Seeds of V. angustifolia, naturally accumulating only RFOs, showed an ability to accumulate exogenously applied D-pinitol or D-chiro-inositol and to form the respective α-D-galactosyl cyclitols. Levels of synthesized galactosides depended on the type and concentration of cyclitol in the feeding solution, and seed maturation stage. However, even a high level of D-pinitol or D-chiro-inositol in the feeding solution caused accumulation of only small amounts of mono-and di-galactosyl pinitols, or tri-galactosyl D-chiro-inositol in seeds of V. angustifolia. Enhanced synthesis of galactosyl cyclitols, mainly mono-and di-galactosides of D-chiro-inositol (fagopyritols), clearly reduced production of verbascose. We suggest that exogenously applied free cyclitols inhibit biosynthesis of tri-and di-galactosides and/or cause substrate competition in enzymes of Vicia species.K Ke ey y W Wo or rd ds s: : D-chiro-inositol, D-pinitol, raffinose family of oligosaccharides, galactosyl pinitols, enzymes, Vicia, seed.

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Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds

Cited by 93 publications

References 18 publications

Factors affecting the nutritional value of pea (<i>Pisum sativum</i>) for broilers

Factors affecting the nutritional value of pea (<i>Pisum sativum</i>) for broilers

Application of phytohormones during seed hydropriming and heat shock treatment on sunflower (Helianthus annuus L.) chilling resistance and changes in soluble carbohydrates

Seed α-D-Galactosides of Selected Vicia Species and Enzymes Involved in Their Biosynthesis

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