Inhibition of superoxide anion-mediated impairment of endothelium by treatment with luteolin and apigenin in rat mesenteric artery

Ma, Xin; Li, Yanfang; Gao, Qin; Ye, Zhiguo; Lu, Xin-Jiang; Wang, Huiping; Jiang, Huidi; Bruce, Iain C.; Xia, Qiang

doi:10.1016/j.lfs.2008.05.010

Cited by 38 publications

(29 citation statements)

References 38 publications

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“…5A). O 2 Ϫ generated by the auto-oxidation of pyrogallol (29,30) did not elicit a vasoactive response in arterioles from either young or old SED rats (Fig. 5B).…”

Section: Scavenging Of Omentioning

confidence: 88%

Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles

Sindler

Reyes

Chen

et al. 2013

Journal of Applied Physiology

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Sindler AL, Reyes R, Chen B, Ghosh P, Gurovich AN, Kang LS, Cardounel AJ, Delp MD, Muller-Delp JM. Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles. J Appl Physiol 114: [681][682][683][684][685][686][687][688][689][690][691][692][693] 2013. First published January 3, 2013; doi:10.1152/japplphysiol.00341.2012.-Exercise training ameliorates age-related impairments in endotheliumdependent vasodilation in skeletal muscle arterioles. Additionally, exercise training is associated with increased superoxide production. The purpose of this study was to determine the role of superoxide and superoxide-derived reactive oxygen species (ROS) signaling in mediating endothelium-dependent vasodilation of soleus muscle resistance arterioles from young and old, sedentary and exercise-trained rats. Young (3 mo) and old (22 mo) male rats were either exercise trained or remained sedentary for 10 wk. To determine the impact of ROS signaling on endothelium-dependent vasodilation, responses to acetylcholine were studied under control conditions and during the scavenging of superoxide and/or hydrogen peroxide. To determine the impact of NADPH oxidase-derived ROS, endothelium-dependent vasodilation was determined following NADPH oxidase inhibition. Reactivity to superoxide and hydrogen peroxide was also determined. Tempol, a scavenger of superoxide, and inhibitors of NADPH oxidase reduced endothelium-dependent vasodilation in all groups. Similarly, treatment with catalase and simultaneous treatment with tempol and catalase reduced endothelium-dependent vasodilation in all groups. Decomposition of peroxynitrite also reduced endothelium-dependent vasodilation. Aging had no effect on arteriolar protein content of SOD-1, catalase, or glutathione peroxidase-1; however, exercise training increased protein content of SOD-1 in young and old rats, catalase in young rats, and glutathione peroxidase-1 in old rats. These data indicate that ROS signaling is necessary for endothelium-dependent vasodilation in soleus muscle arterioles, and that exercise traininginduced enhancement of endothelial function occurs, in part, through an increase in ROS signaling. hydrogen peroxide; superoxide; peroxynitrite; nitric oxide; acetylcholine ENDOTHELIAL FUNCTION IN THE skeletal muscle resistance vasculature declines with age primarily due to decreased nitric oxide (NO) bioavailability (10,38,51,55). In feed arteries from soleus muscle, reductions in NO-dependent vasodilation are accompanied by reduced expression of endothelial NO synthase (eNOS) (65). In contrast, our laboratory has previously reported that NO-mediated vasodilation of soleus muscle arterioles declines with advancing age, despite an increase in eNOS protein levels (51). Thus the age-related decline in bioavailability of NO may be dependent on numerous other factors that regulate both NO production and degradation. eNOS activity, and subsequent NO production, is regulated by availability of substrate and cofactors, by protein-...

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“…5A). O 2 Ϫ generated by the auto-oxidation of pyrogallol (29,30) did not elicit a vasoactive response in arterioles from either young or old SED rats (Fig. 5B).…”

Section: Scavenging Of Omentioning

confidence: 88%

Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles

Sindler

Reyes

Chen

et al. 2013

Journal of Applied Physiology

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“…An infusion is taken internally in the treatment of bronchial complaints and coughs, insomnia, poor digestion, nervous digestive disorders and minor painful conditions. The flowers are also used in the treatment of jaundice and -in very small quantities-as a sleep-inducing drug [9,10].Papaver rhoeas plant extract contains a lot of flavonoids: kaempferol, quercetin, luteolin and hypolaetin andAntioxidant activity of the kaempferol [12][13], quercetin [14][15] and luteolin [16] was already demonstrated. Total phenolic compounds (9.73 -19.91 mg GAE/g) and total flavonoids (7.904-11.45 mg QE/g) were determined in fresh petals [17].…”

mentioning

confidence: 99%

“…Antioxidant activity of the kaempferol [12][13], quercetin [14][15] and luteolin [16] was already demonstrated. Total phenolic compounds (9.73 -19.91 mg GAE/g) and total flavonoids (7.904-11.45 mg QE/g) were determined in fresh petals [17].…”

mentioning

confidence: 99%

Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

Hašplová¹,

Hudecová²,

Miadoková³

et al. 2011

neo

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Varied medicinal plants are known as a source of natural phytochemicals with antioxidant activities that can protect organisms from oxidative stress and from various chronic diseases. Papaver rhoeas has a long history of medicinal usage, especially for ailments in adults and children. The possible cytotoxicity, genotoxicity and potential antioxidant effect of plant extract isolated from flowers of Papaver rhoeas was investigated in human lymfoblastoid cell line (TK6). Antioxidant activity of this extract was determined using the DPPH assay. The plant extract exhibited dose dependent free radical scavenging ability. The growth activity assay was used for determination of cytotoxicity. To assess potential genotoxicity the comet assay was used. The lower extract concentrations (0.25 and 0.5 mg/ml) neither exerted cytotoxic, nor genotoxic effects in TK6 cells but they stimulated cell proliferation. The concentration 25 mg/ml scavenged almost 85% of DPPH free radical. On the other hand, this concentration had strong cytotoxic and genotoxic effect on TK6 cells.The balance between beneficial and harmful effects should be always considered when choosing the effective dose. Plants are universally recognized as a vital part of the world's natural heritage and up to 80% of the population relies on plants for their contribution to a primary healthcare. The use of plants in traditional medicine is widespread. Many plants still represent a large source of natural antioxidants that might serve as leads for the development of novel drugs. It is commonly accepted that due to oxidative stress, reactive oxygen species (ROS) such as superoxid, hydroxyl and peroxyl radicals are generated [1]. Recently much attention has been given to the natural phytochemicals with antioxidant, antimutagenic and anticarcinogenic activities [2][3][4][5][6][7][8]. These compounds are of great importance for chemoprevention and potential cancer biotherapy.Papaveraceae is a family of flowering plants. The flowers of poppy are chiefly employed as a mild pain reliever and as a treatment for irritable coughs; it also helps to reduce nervous over-activity [9]. The flowers and petals are anodyne, expectorant, hypnotic, slightly narcotic and sedative. An infusion is taken internally in the treatment of bronchial complaints and coughs, insomnia, poor digestion, nervous digestive disorders and minor painful conditions. The flowers are also used in the treatment of jaundice and -in very small quantities-as a sleep-inducing drug [9,10].Papaver rhoeas plant extract contains a lot of flavonoids: kaempferol, quercetin, luteolin and hypolaetin andAntioxidant activity of the kaempferol [12][13], quercetin [14][15] and luteolin [16] was already demonstrated. Total phenolic compounds (9.73 -19.91 mg GAE/g) and total flavonoids (7.904-11.45 mg QE/g) were determined in fresh petals [17].Besides flavonoids and their glycosides Papaveraceae family are known for the presence of alkaloids as morphine, codeine, narcotine, thebaine and papaverine [8,18,19].The aim of this study...

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“…We selected the appropriate incubation concentration of PYG at 300 M as a source of exogenous oxidant stress in the arterial rings, and at this concentration, superoxide anions impaired, but did not abolish the endothelium-dependent relaxation to ACh. We found that acute exposure of arterial rings to PYG resulted in an impairment of subsequent endothelium-dependent, EDHF-mediated relaxation to ACh (Ma et al, 2008). Usually, endothelial cell hyperpolarization is an upstream event of this EDHF-mediated relaxation, and the hyperpolarization is triggered when [Ca 2+ ] i is increased and the small and/or intermediate-conductance calcium-activated K + channels are activated (Edwards et al, 1998).…”

Section: Discussionmentioning

confidence: 94%

“…It is a commonly used traditional Chinese medicinal herb and approved as a health food by the State Ministry of Health of China. Our previous studies showed that ROS-induced impairment of vasodilation is greatly improved by oral administration of total flavonoids of Flos Chrysanthemi (TFFC) (Ma et al, 2008); it also increases the superoxide dismutase (SOD) activity in the heart during ischemia and reperfusion . Furthermore, Flos Chrysanthemi has a beneficial effect on coronary heart diseases Xu et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of superoxide anion-mediated impairment of endothelium by treatment with luteolin and apigenin in rat mesenteric artery

Cited by 38 publications

References 38 publications

Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles

Age and exercise training alter signaling through reactive oxygen species in the endothelium of skeletal muscle arterioles

Biological activity of plant extract isolated from Papaver rhoeas on human lymfoblastoid cell line

Total flavonoids of Flos Chrysanthemi protect arterial endothelial cells against oxidative stress

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