Inhibition of the Epstein-Barr Virus Lytic Cycle by Andrographolide

Lin, Tsuey-Pin; Chen, Shih‐Ying; Duh, Pin-Der; Chang, Li-Kwan; Liu, Yen-Ni

doi:10.1248/bpb.31.2018

Cited by 84 publications

(73 citation statements)

References 39 publications

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“…Thus, inhibition of the lytic cycle of EBV could be a useful strategy to aid in the reduction of the risk of such diseases. A similar study focusing on the potential of andrographolide as an anti-EBV drug based on its inhibition of the lytic cycle of EBV was reported by Lin et al (2008). However, that study was based on the inhibitions of the lytic genes encoding Rta, Zta, and EA-D rather than assessing the amounts of cell-free EBV DNA.…”

Section: Discussionmentioning

confidence: 92%

“…A possible target action of an antiviral compound against EBV is by inhibiting the lytic cycle, which will prevent the virus from producing mature viral particles. For instance, andrographolide from a medicinal plant inhibits the transcriptions of BRLF1 and BZLF1, two immediate early genes involved in the lytic cycle of EBV (Lin et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, a compound from licorice root, glycyrrhizic acid, inhibits EBV replication in superinfected Raji cells while acerogenin from Aceraceae inhibits the expression of EBV early antigen induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in mice (Lin, 2003;Akihisa et al, 2006). Ethanolic extract and andrographolid from Andrographis paniculata inhibit the expressions of Rta, Zta, and EA-D proteins of EBV in P3HR-1 cells (Lin et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

Kok

Chu

Phang

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against Epstein-Barr virus (EBV) in three Burkitt's lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC 50 <0.01 µg/ml) with a high therapeutic index (>28 000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC 50 =2.9 µg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA (EC 50 =1.38 µg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

Kok

Chu

Phang

et al. 2011

J. Zhejiang Univ. Sci. B

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“…Phytochemical studies on APN have found the principal bioactive compound andrographolide [7] and many other constituents, including diterpenoids, flavonoids, quinic acids, xanthones [8, 9], and noriridoids [10, 11]. Based on the abundant bioactive constituents, APN showed many salutary effects, such as anticancer [12], hepatoprotective [13], antiviral [14, 15], antipyretic and analgesic [16] effects, etc . Interestingly, the immunomodulatory [17], antioxidant [18], anti-inflammatory [19] and antimicrobial [20, 21] activities of APN were also reported, which may suggest the clinical use of APN in treatment for PID.…”

Section: Introductionmentioning

confidence: 99%

The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

Zou

Xiao

Wen

et al. 2016

BMC Complement Altern Med

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Background Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism.MethodsThirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted.ResultsAPN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway.ConclusionAPN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.

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“…The A. paniculata ethanol extract and andrographolide inhibit the expression of Epstein-Barr virus (EBV) lytic proteins during the viral lytic cycle in P3HR1 cells, an oral lymphoma cell line latently infected by EBV. Andrographolide inhibits the production of mature viral particles and is not toxic to P3HR1 cells [82]. …”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)

2010

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Andrographis paniculata (Burm. f.) Nees (Acanthaceae) is a medicinal plant used in many countries. Its major constituents are diterpenoids, flavonoids and polyphenols. Among the single compounds extracted from A. paniculata, andrographolide is the major one in terms of bioactive properties and abundance. Among the andrographolide analogues, 14-deoxy-11,12-didehydroandrographolide is immunostimulatory, anti-infective and anti-atherosclerotic; neoandrographolide is anti-inflammatory, anti-infective and anti-hepatotoxic; 14-deoxyandrographolide is immunomodulatory and anti-atherosclerotic. Among the less abundant compounds from A. paniculata, andrograpanin is both anti-inflammatory and anti-infective; 14-deoxy-14,15-dehydroandrographolide is antiinflammatory; isoandrographolide, 3,19-isopropylideneandrographolide and 14-acetylandrographolide are tumor suppressive; arabinogalactan proteins are anti-hepatotoxic. The four flavonoids from A. paniculata, namely 7-Omethylwogonin, apigenin, onysilin and 3,4-dicaffeoylquinic acid are anti-atherosclerotic.

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Inhibition of the Epstein-Barr Virus Lytic Cycle by Andrographolide

Cited by 84 publications

References 39 publications

Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)

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