Inhibition of the Growth and Development of Sclerotinia sclerotiorum (Lib.) De Bary by Combining Azoxystrobin, Penicillium chrysogenum VKM F-4876d, and Bacillus Strains

Yaderets, V. V.; Karpova, Nataliya V.; Глаголева, Е. В.; Ovchinnikov, A. I.; Petrova, Kseniya S.; Dzhavakhiya, V. V.

doi:10.3390/agronomy11122520

Cited by 9 publications

(6 citation statements)

References 58 publications

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“…Earlier, we determined the DMP ability to suppress the growth of Sclerotinia sclerotiorum (Lib.) de Bary [44], some pathogenic Fusarium fungi (Fusarium oxysporum, F. graminearum, F. avenaceum, and F. culmorum) [31], and Alternaria solani [45] when used both individually and in combination with some fungicides.…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of the Growth of Botrytis cinerea by Penicillium chrysogenum VKM F-4876D Combined with Fludioxonil-, Difenoconazole-, or Tebuconazole-Based Fungicides

Hatem,

Yaderets,

Karpova

et al. 2023

Agronomy

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Botrytis cinerea, causing grey mold, is a dangerous plant pathogen able to infect agricultural crops during the whole production cycle, including storage and transportation. A wide set of pathogenicity factors, high ecological plasticity, and universality of propagation and spreading of this fungus significantly complicate the control of this pathogen. A rapid increase in pathogen tolerance to fungicides dictates the necessity of developing antiresistant protection strategies, which include the use of biopreparations based on antagonistic microorganisms or their metabolites. The purpose of the study was to evaluate the antifungal activity of a dry biomass of P. chrysogenum VKM F-4876D (DMP), both individually and in combination with tebuconazole-, fludioxonil-, or difenoconazole-containing compounds recommended to control grey mold, in relation to B. cinerea isolated from grape samples. A water suspension of DMP was added to the PDA medium at a concentration of 1.0, 2.5, 5.0, 7.5, and 10.0 g/L. The pathogen growth inhibition was evaluated after 3, 7, and 14 days of cultivation; fungal cultures grown on DMP-free medium were used as a control. The resulting effective DMP concentration was 2.5 g/L. The effective concentrations of fungicides included in the study were determined to be 0.5 mg/L (tebuconazole), 0.1 g/L (difenoconazole), and 0.04 mg/L (fludioxonil). Combining DMP (2.5 g/L) with tebuconazole, difenoconazole, or fludioxonil (all taken at the effective concentrations) resulted in pathogen growth inhibition after 7 days of incubation by 86.5, 85.6, and 84.6%, respectively. Among all studied variants, the DMP (2.5 g/L) + difenoconazole (1.0 mg/L) combination provided the most efficient control of B. cinerea development under in vitro conditions: even after 14 days of incubation, the pathogen growth suppression remained at the level of 51.3%, whereas the DMP combination with tebuconazole or fludioxonil provided only 28.5 and 37.4%, respectively. The obtained results show good prospects for the efficient control of grey mold development, together with the reduction of pesticide loads on agrobiocoenoses and the prevention of the emergence of new resistant forms of plant pathogens.

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Section: Discussionmentioning

confidence: 99%

“…In our previous study, we identified 6-demethylmevinolin, or mevastatin, as a component of the culture broth of F-4856D [44]. This compound possesses antifungal properties due to its ability to inhibit the biosynthesis of ergosterol, a basic cell wall component in fungi [46,47].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Growth of Botrytis cinerea by Penicillium chrysogenum VKM F-4876D Combined with Fludioxonil-, Difenoconazole-, or Tebuconazole-Based Fungicides

Hatem,

Yaderets,

Karpova

et al. 2023

Agronomy

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“…DMP obtained from VKM F-4876D has been previously shown to suppress the growth and development of Sclerotinia sclerotiorum (Lib.) de Bary [31] and several plant pathogenic Fusarium fungi (F. oxysporum, F. graminearum, F. avenaceum, and F. culmorum) [30] when used both alone and in combination with azoxystrobin. The current study showed that DMP also possesses a significant antifungal activity against A. solani.…”

Section: Discussionmentioning

confidence: 99%

“…The past study showed the ability of this strain to suppress the growth and development of Sclerotinia sclerotiorum (Lib.) de Bary and plant pathogenic Fusarium fungi (F. oxysporum, F. graminearum, F. avenaceum, and F. culmorum) under the conditions of both the combination of a dry mycelium biomass (DMP) of P. chrysogenum F-4876D with azoxystrobin and the use of DMP only [30,31]. Thus, taking into account the significance of potato as an agricultural crop in Russia and on a global scale, it was interesting to study the antifungal activity of DMP, used alone or in combination with several commercial fungicides (azoxystrobin-, tebuconazole-, fludioxonil-, and difenoconazole-based formulations used for potato protection) to control A. solani.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the Growth and Development of Potato Early Blight Pathogen (Alternaria solani) by Combining Penicillium chrysogenum VKM F-4876D with Some Strobilurin-, Triazole-, and Phenylpyrrole-Based Fungicides

et al. 2022

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The biological control of fungal crop diseases based on the use of micro-organisms or their metabolites is a promising environmentally friendly alternative to common fungicide-based technologies. Penicillium fungi which synthesize various biologically active compounds with a wide range of antimicrobial activity are of special interest as potential producers of antifungal biopreparations. The purpose of this study was the evaluation of the antifungal activity of a dry biomass of the Penicillium chrysogenum VKPM F-4876D strain (DMP), both individually and combined with commercial azoxystrobin-, fludioxonil-, difenoconazole-, and tebuconazole-based fungicides commonly used to control early blight, towards Alternaria solani, an infectious agent of this potato disease. The study was performed under in vitro (cultivation on solid and liquid media) and in vivo (detached potato leaves) conditions. The cultivation of A. solani on agarized medium supplemented with 5 g/L of DMP caused significant morphological changes in pathogen colonies, whereas 7.5 g/L of DMP resulted in complete suppression of pathogen development. DMP addition to the liquid culture of A. solani significantly increased electrolyte release, i.e., impaired the cell membrane integrity in the pathogen. Combining DMP (1–2 g/L) with fungicides at a dose range of 0.01–10 mg/L resulted in significant in vitro growth inhibition of A. solani. The best result was obtained for the combination of DMP (1 g/L) with fludioxonil (1–5 mg/L) or tebuconazole (10 mg/L): a significant and prolonged antifungal effect (96–97% growth inhibition) was observed during the whole 14-day period of observation. The treatment of detached potato leaves with 1 g/L of DMP prior to artificial infection with A. solani resulted in a four-fold increase in the number of uninfected leaves and a two-fold reduction in the number of severely infected leaves compared to the control. These results demonstrate the possibility to develop an efficient environmentally friendly approach to manage potato early blight and provide prerequisites for the reduction in pesticide load on agrobiocoenoses, thus contributing to the restoration of ecological balance and the prevention of the emergence and spread of resistant pathogen strains.

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“…Consequently, this impedes mycelial growth and serves as a preventive measure against the development and proliferation of pathogenic spores [13]. It is able to block the development of ascospores that can be considered as an indirect way to control S. sclerotiorum spreading, since sclerotia, which provides the development of the apothecium and ascospores, may initiate new disease development cycles, thus complicating the control of this pathogen [14]. It can also be used to control Ascochyta, Powdery mildew, and other diseases transmitted through the foliar and soil [15].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Azoxystrobin-Zinc Metal–Organic Framework/Biomass Charcoal Composite Materials and Application in the Prevention and Control of Gray Mold in Tomato

Han,

Qian,

et al. 2023

IJMS

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In order to reduce the use of fungicide and ensure food safety, it is necessary to develop fungicide with low toxicity and high efficiency to reduce residues. Azoxystrobin (AZOX), which is derived from mushrooms, is an excellent choice. However, conventional AZOX release is difficult to regulate. In this paper, a pH-responsive fungicide delivery system for the preparation of AZOX by impregnation method was reported. The Zinc metal–organic framework/Biomass charcoal (ZIF-8/BC) support was first prepared, and subsequently, the AZOX-ZIF-8/BC nano fungicide was prepared by adsorption of AZOX onto ZIF-8/BC by dipping. Gray mold, caused by Botrytis cinerea, is one of the most important crop diseases worldwide. AZOX-ZIF-8/BC could respond to oxalic acid produced by Botrytis cinerea to release loaded AZOX. When pH = 4.8, it was 48.42% faster than when pH = 8.2. The loading of AZOX on ZIF-8/BC was 19.83%. In vitro and pot experiments showed that AZOX-ZIF-8/BC had significant fungicidal activity, and 300 mg/L concentration of AZOX-ZIF-8-BC could be considered as a safe and effective control of Botrytis cinerea. The above results indicated that the prepared AZOX-ZIF-8/BC not only exhibited good drug efficacy but also demonstrated pH-responsive fungicide release.

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Inhibition of the Growth and Development of Sclerotinia sclerotiorum (Lib.) De Bary by Combining Azoxystrobin, Penicillium chrysogenum VKM F-4876d, and Bacillus Strains

Cited by 9 publications

References 58 publications

Inhibition of the Growth of Botrytis cinerea by Penicillium chrysogenum VKM F-4876D Combined with Fludioxonil-, Difenoconazole-, or Tebuconazole-Based Fungicides

Inhibition of the Growth of Botrytis cinerea by Penicillium chrysogenum VKM F-4876D Combined with Fludioxonil-, Difenoconazole-, or Tebuconazole-Based Fungicides

Inhibition of the Growth and Development of Potato Early Blight Pathogen (Alternaria solani) by Combining Penicillium chrysogenum VKM F-4876D with Some Strobilurin-, Triazole-, and Phenylpyrrole-Based Fungicides

Preparation of Azoxystrobin-Zinc Metal–Organic Framework/Biomass Charcoal Composite Materials and Application in the Prevention and Control of Gray Mold in Tomato

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