Inhibition of the Stomatal Blue Light Response by Verapamil at High Concentration

Shimazaki, Ken-icbiro; Tominaga, Misumi; Shigenaga, Ayako

doi:10.1093/oxfordjournals.pcp.a029230

Cited by 19 publications

(10 citation statements)

References 34 publications

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“…The kinetics of the blue light-dependent acidification of the external medium closely matched that observed for stomatal opening in intact leaves. It was postulated that blue light activates the plasma membrane H + -ATPase via Ca 2 + -dependent pathways, probably also including protein phosphorylation [108][109][110]. The H + extrusion observed by Shimazaki et al [107] was in accordance with electrophysiological studies demonstrating membrane hyperpolarization in response to blue light [97].…”

Section: Blue Light and Membrane Hyperpolarizationmentioning

confidence: 62%

Ion channels in plant signaling

Zimmermann

Ehrhardt

Plesch

et al. 1999

CMLS, Cell. Mol. Life Sci.

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Plant ion channel activities are rapidly modulated in response to several environmental and endogenous stimuli such as light, pathogen attack and phytohormones. Electrophysiological as well as pharmacological studies provide strong evidence that ion channels are essential for the induction of specific cellular responses, implicating their tight linkage to signal transduction cascades. Ion channels propagate signals by modulating the membrane potential or by directly affecting cellular ion composition. In addition, they may also be effectors at the end of signaling cascades, as examplified by ion channels which determine the solute content of stomatal guard cells. Plant channels are themselves subject to regulation by a variety of cellular factors, including calcium, pH and cyclic nucleotides. In addition, they appear to be regulated by (de)-phosphorylation events as well as by direct interactions with cytoskeletal and other cellular proteins. This review summarizes current knowledge on the role of ion channels in plant signaling.

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Section: Blue Light and Membrane Hyperpolarizationmentioning

confidence: 62%

Ion channels in plant signaling

Zimmermann

Ehrhardt

Plesch

et al. 1999

CMLS, Cell. Mol. Life Sci.

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“…Calmodulin antagonists inhibited both blue-light-dependent H + pumping and stomatal opening (158). The pumping was inhibited by verapamil, a Ca 2+ channel blocker, albeit at high concentrations (160). The H + pumping was inhibited reversibly by caffeine, which releases Ca 2+ from intracellular stores, and by inhibitors of endoplasmic reticulum Ca 2+ -ATPase.…”

Section: Vfpip a Protein Interacting Withmentioning

confidence: 99%

Light Regulation of Stomatal Movement

Shimazaki

Doi²,

Assmann

et al. 2007

Annu. Rev. Plant Biol.

785

765

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Stomatal pores, each surrounded by a pair of guard cells, regulate CO2 uptake and water loss from leaves. Stomatal opening is driven by the accumulation of K+ salts and sugars in guard cells, which is mediated by electrogenic proton pumps in the plasma membrane and/or metabolic activity. Opening responses are achieved by coordination of light signaling, light-energy conversion, membrane ion transport, and metabolic activity in guard cells. In this review, we focus on recent progress in blue- and red-light-dependent stomatal opening. Because the blue-light response of stomata appears to be strongly affected by red light, we discuss underlying mechanisms in the interaction between blue-light signaling and guard cell chloroplasts.

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“…Here we report genetic, biochemical and signaling network mechanisms that underpin this cellular response. In the absence of ABA, Ca 2+ responsiveness is inhibited by PP2Cs, thereby preventing responses to unrelated stomatal opening-mediating stimuli (Irving et al, 1992;Shimazaki et al, 1992;Curvetto et al, 1994;Shimazaki et al, 1997;Cousson and Vavasseur, 1998;Young et al, 2006) and also spontaneous Ca 2+ elevations (Young et al, 2006;Siegel et al, 2009). As PP2Cs inhibit OST1 and also down-regulate SLAC1 directly, this network not only enables stimulus specific activation of SLAC1, but also provides a tight off switch via PP2C-catalyzed dephosphorylation of (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

“…Simplified schematic model for Ca 2+ -specificity mechanism within ABA-dependent SLAC1 activation in guard cells. (A) Without ABA, Ca 2+ elevations that can also function in stomatal opening responses (Irving et al, 1992;Shimazaki et al, 1992;Curvetto et al, 1994;Shimazaki et al, 1997;Cousson and Vavasseur, 1998;Young et al, 2006) and spontaneous or un-specifically induced Ca 2+ transients (Allen et al, 1999b;Klüsener et al, 2002;Young et al, 2006;Yang et al, 2008;Siegel et al, 2009) do not lead to S-type anion channel (SLAC1) activation as PP2C protein phosphatases directly negatively regulate SLAC1 activation. (B) In the presence of ABA this SLAC1 inhibition is released, OST1 and CPKs phosphorylate, and thereby activate the channel.…”

Section: Discussionmentioning

confidence: 99%

Author response: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells

Brandt

Munemasa

Wang

et al. 2014

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A central question is how specificity in cellular responses to the eukaryotic second messenger Ca 2+ is achieved. Plant guard cells, that form stomatal pores for gas exchange, provide a powerful system for in depth investigation of Ca 2+-signaling specificity in plants. In intact guard cells, abscisic acid (ABA) enhances (primes) the Ca 2+-sensitivity of downstream signaling events that result in activation of S-type anion channels during stomatal closure, providing a specificity mechanism in Ca 2+-signaling. However, the underlying genetic and biochemical mechanisms remain unknown. Here we show impairment of ABA signal transduction in stomata of calcium-dependent protein kinase quadruple mutant plants. Interestingly, protein phosphatase 2Cs prevent non-specific Ca 2+-signaling. Moreover, we demonstrate an unexpected interdependence of the Ca 2+-dependent and Ca 2+-independent ABA-signaling branches and the in planta requirement of simultaneous phosphorylation at two key phosphorylation sites in SLAC1. We identify novel mechanisms ensuring specificity and robustness within stomatal Ca 2+-signaling on a cellular, genetic, and biochemical level.

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Inhibition of the Stomatal Blue Light Response by Verapamil at High Concentration

Cited by 19 publications

References 34 publications

Ion channels in plant signaling

Ion channels in plant signaling

Light Regulation of Stomatal Movement

Author response: Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells

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