Inhibition of tomato bushy stunt virus infection using a quercetagetin flavonoid isolated from Centaurea rupestris L.

Rusak, Gordana; Krajačić, Mladen; Pleše, Nada

doi:10.1016/s0166-3542(97)00045-4

Cited by 40 publications

(20 citation statements)

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“…Several species from the genus Centaurea are interesting from medicinal and pharmacological perspective, and thus recently the subject of intensive research [3][4][5][6][7][8][9][10][11][12][13][14]. Since structural and phytochemical characteristics of C. sadleriana were previously unknown, our work highlights this information.…”

Section: Discussionmentioning

confidence: 98%

“…As Centaurea species were generally classified as essential oil-poor plants, the investigations of these and antioxidant properties are rather scarse. However, antiinflammatory, antimicrobial, antifungal and cytotoxic activities had been found for extracts or natural products of some of the Centaurea species [3,[5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance

et al. 2013

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The lamina, main vein and peduncle anatomical properties of Centaurea sadleriana Janka plants from two populations, were examined using light and scanning electron microscopy. The indumentum was comprised of glandular and non-glandular trichomes of two types. The leaves were amphistomatic, isolateral, with strongly developed palisade tissue. Secretory ducts were observed along the phloem or sclerenchyma of large vascular bundles. Collenchyma alternated with chlorenchyma in the main vein and peduncle. Large groups of strongly lignified sclerenchyma were present along the phloem of peduncle vascular bundles. These features, together with thickened walls of epidermal cells and cuticle, numerous trichomes and thick-walled parenchyma in the perimedullar zone, were perceived as a xeromorphic peduncle structural adaptation. Non-enzymatic antioxidant compounds of phenolic origin were detected in small amounts and their respective content was higher in leaves compared to inflorescences. Compounds of phenolic orgin showed positive correlation with total potenial of antioxidant activity indicated by the DPPH assay. Greater total quantity of polyphenols and tannins was detected in leaves of plants from Zobnatica locality, while leaves of plants from Rimski Sanac were characterized by higher content of total flavonoids and proantocyanidins. Phytochemical analysis showed that dominant secondary biomolecules in inflorescences were phenolic pigments including anthocyanins and leucoanthocyanins, and free quinones in leaves.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance

et al. 2013

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“…[4,6] Members of Centaurea species were proposed to have antimicrobial, [5,[7][8][9][10] antifungal, [8,11] anti-inflammatory, [12] anti-ulcerogenic, [13] antioxidant, [14][15][16][17] antiplasmodial, [18] antiprotozoal, [8] antiviral [19] and cytotoxic [18,[20][21][22] activities. C. calcitrapa which grows up on northwestern Anatolia is used (2-6% infusion) as a fever reducer.…”

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confidence: 99%

Total Phenolic Contents, Antioxidant Activities Cytotoxicity of Three Centaurea Species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa C. spicata

Erol-Dayi

Pekmez

Bona

et al. 2011

Free Radicals and Antioxidants

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Background: Many species of the genus Centaurea have been reported to be used in Anatolian folk medicine. In the present study, methanolic and aqueous extracts of three species of Centaurea L. (C. calcitrapa subsp. calcitrapa, C. ptosimopappa and C. spicata) have been assessed for antioxidant and cytotoxic activities. Materials and Methods: Antioxidant activity was investigated by DPPH and superoxide anion scavenging and anti-lipid peroxidation assays. Cytotoxic effect on HeLa and Vero cells were investigated by MTT assay. Results: Superoxide anion scavenging activities of aqueous extracts were higher than those of methanolic extracts, and the highest activity was detected in the aqueous extracts of C. calcitrapa subsp. calcitrapa with the IC 50 value of 25.91 µg/mL. The methanolic extract of C. ptosimopappa showed the strongest anti-lipid peroxidation activity. Total phenolic contents (mg gallic acid equivalent per gram) were ranged between 17.25 and 120.50 and the highest value was detected in aqueous extract of C. ptosimopappa. The highest cytotoxic activity was observed in the methanolic extract of C. calcitrapa subsp. calcitrapa on both cell lines with the IC 50 values of <100 µg/mL. Conclusions: According to the results of activity tests, the selected Centaurea species, especially C. calcitrapa subsp. calcitrapa can be regarded as a potential source of natural antioxidants in the prevention and treatment of diseases in which oxidants or free radicals are implicated.Key-words: Antioxidant activity; Centaurea; Cytotoxicity; Free radical scavenging.Key Messages: The selected Centaurea species showed an antioxidant activity and very low cytotoxic activity. Thus, Centaurea species might be a good candidate for further studies targeting the development of new antioxidants.

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“…Our research encompassed seven flavonoids (myricetin, quercetin, galangin, kaempferol, luteolin, naringenin and MQ) and three phenolic acids (caffeic, p-coumaric and cinnamic acid) which were already found in genus Centaurea and in the family Asteraceae. 14,33,34 Our analyses revealed that MQ was the predominant flavonoid in flowers and its amount in flowers was approximately two orders of the magnitude higher than in the leaves of naturally growing plants, in shoots grown in vitro on MS and in tissue line G. MQ was not detected in UC while in roots and in shoots grown in vitro on MS ½ the content of MQ was below the limit of quantification. Furthermore, after hydrolysis of extracts, we were able to detect traces of myricetin and kaempferol in flower extracts and in extracts from shoots grown in vitro.…”

Section: Resultsmentioning

confidence: 82%

“…Quercetin also induced resistance to Potato virus X (PVX) in Chenopodium quinoa 9 and was active against Tomato ringspot virus 10 and Cucumber mosaic virus, 11 while it acted synergistically with ribavirin against Apple stem grooving virus. 12 Flavonoid quercetagetin 3'-methylether-7-O-ß-D-glucopyranoside (MQ), isolated from the inflorescences of Croatian endemic species Centaurea rupestris L. 13 interferes with the initiation of Tomato bushy stunt virus (TBSV) infection 14 and it also has antibacterial and antifungal activities. 15 The diversity of other phenolics in C. rupestris and their possible antiphytoviral activity has never been tested before.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

Ćurković‐Perica¹,

Likić²,

Rusak³

2014

Croat. Chem. Acta

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Abstract. Quantitative and qualitative analysis of phenolic compounds in tissue extracts from Centaurea rupestris L. as well as their antiphytoviral activity against Tomato bushy stunt virus was performed. Extracts of flowers, leaves and roots from C. rupestris growing in the wild, as well as extracts from shoots, tissue consisting of callus and shoots and undifferentiated callus grown in vitro, were tested. Between tested extracts predominantly quantitative and only several qualitative differences in phenolics were detected by high performance liquid chromatography. The highest amounts of quercetagetin 3'-methylether-7-O-ß-D-glucopyranoside and quercetin were detected in flowers while in leaves the highest amounts of luteolin, caffeic and p-coumaric acid were detected. Except for roots, antiphytoviral activity of all other extracts was high, inducing virus inhibition, ranging from 43 to 90 %. Simultaneous application of quercetin, caffeic or p-coumaric acid with virus decreased the number of lesions indicating that these substances contribute to the antiphytoviral activity of C. rupestris extracts. (doi: 10.5562/cca2272)

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Inhibition of tomato bushy stunt virus infection using a quercetagetin flavonoid isolated from Centaurea rupestris L.

Cited by 40 publications

References 19 publications

Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance

Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance

Total Phenolic Contents, Antioxidant Activities Cytotoxicity of Three Centaurea Species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa C. spicata

Phenolic Compounds in Centaurea rupestris Tissues and Their Antiphytoviral Activity

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