Inhibition of VEGF expression in tongue squamous cancer cells via RNA interference silencing of iNOS gene

Yan, Lan; Chen, Wei‐liang; Zeng, Su; Wang, Yongjie; Bai, Zhi-Bao

doi:10.1016/j.ijom.2009.01.017

Cited by 4 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The emerging class of RNA interference (RNAi) therapeutics is a novel approach to treat human diseases. Considering the fact that the RNAi is a natural cellular mechanism for regulating gene expression, these molecules have been gaining attention as useful tools for treatment of tumors [270][271][272][273][274][275]. Briefly, long double stranded ribonucleic acid (dsRNA) molecules are processed to small interfering RNA (siRNA) via the cytoplasmic enzyme Dicer.…”

Section: Improving the Delivery Of Short Hairpin Rna For Cancer Treat...mentioning

confidence: 99%

Applications of Nanosystems to Anticancer Drug Therapy (Part II. Dendrimers, Micelles, Lipid-based Nanosystems)

Ruiz¹,

Gantner²,

Talevi

2013

PRA

View full text Add to dashboard Cite

The great efforts of many researchers have brought down some of the barriers that exist to turn a good in vitro compound into a potential in vivo drug. The advent of pharmaceutical nanotechnology has allowed an arsenal of drugs with poor stability, low solubility, high off-target toxicity and other disadvantageous features, to be accessible as pharmaceutical products that could be administered to a patient. Nanotechnology was introduced in drug delivery very long ago, but has flourished with unprecedented intensity during the last twenty years and now a diversity of nano-based preparations are at clinical stage of development or already available in the market. Undoubtedly, nanotechnology plays a key role in future pharmaceutical development and pharmacotherapy. In the first part of this review, we have already discussed recent (2008-2012) patents on linear polymer-based nanosystems (nanogels, nanospheres and nanocapsules) applications to cancer therapy. Here, we have expanded such analysis to branched polymers (dendrimers), self-assembling nanomicelles and lipid-based nanocarriers.

show abstract

Section: Improving the Delivery Of Short Hairpin Rna For Cancer Treat...mentioning

confidence: 99%

Applications of Nanosystems to Anticancer Drug Therapy (Part II. Dendrimers, Micelles, Lipid-based Nanosystems)

Ruiz¹,

Gantner²,

Talevi

2013

PRA

View full text Add to dashboard Cite

show abstract

“…5,6 There have been several investigations that have concentrated on examining the functions of iNOS in addition to VEGF in oral squamous cell carcinoma (OSCC) also their contribution in the regulation of various biological processes responsible for the growth of tumors. 7 These processes include the host immune response, cell proliferation, programmed cell death resistance, and the development of new vasculature within tumor environments. 8 VEGF activation in epithelial cells, brought about by a range of stimuli, appears to contribute to the control of tumor vascularity.…”

Section: Introductionmentioning

confidence: 99%

Colocalization of VEGF and iNOS in Parenchymal and Stromal Cells of Oral Squamous Cell Carcinoma

Essa

2024

Egyptian Dental Journal

View full text Add to dashboard Cite

Background and objectives: Tumor angiogenesis and inflammation perception are intricately linked to cancer progression. Vascular endothelial growth factor (VEGF) controls distinct procedures in angiogenesis of pathological conditions in metabolic functions of cancer cells with the inflammatory cytokine inducible nitric oxide synthase (iNOS) in parenchymal together with stromal cells of oral squamous cell carcinoma (OSCC) as well as assessing microvessel density (MVD).Methods: Immunohistochemistry was applied to analyze VEGF, iNOS as well as CD31 expression within 31 OSCCs paraffin blocks, which grouped into 13 well differentiated, 10 moderately differentiated, and 8 poorly differentiated (paraffin blocks OSCC).Results: Both VEGF and iNOS exhibit strong expression in both parenchymal and stromal cells in all grades of OSCC together with increased MVD. Conclusion:Enhanced expression of VEGF and iNOS together with MVD potentially correlate with OSCC grades.

show abstract

“…Research has shown that iNOS and VEGF are involved in tumour angiogenesis and also is highly expressed in SACC . Moreover, iNOS can regulate VEGF expression in tumour cells . However, whether iNOS has the same regulatory mechanism in SACC via VEGF is still not clear.…”

Section: Introductionmentioning

confidence: 99%

A study on the inhibition of VEGF expression in salivary gland adenoid cystic carcinoma cells via iNOS gene RNAi in vitro

Yang

Liang

Yang

et al. 2014

J Oral Pathology Medicine

View full text Add to dashboard Cite

In this study, we aimed to explore the effect of inducible nitric oxide synthase (iNOS) on vascular endothelial growth factor (VEGF) expression in salivary gland adenoid cystic carcinoma (SACC). Using RNAi, we transfected chemically synthesised iNOS siRNA into ACC-M cells (a highly metastatic adenoid cystic carcinoma cell line) and detected the change in the gene and protein expression levels of iNOS and VEGF by qRT-PCR and Western blotting. A transwell invasiveness assay was used to examine the changes in invasive ability of ACC-M cells. Cell growth was determined using a CCK-8 assay. Apoptosis and cell-cycle phases were detected by flow cytometry. We found that silencing iNOS down-regulated the expression of VEGF and then inhibited cell growth and invasiveness of SACC cells, while it increased apoptosis. Therefore, we concluded that iNOS can regulate VEGF expression and iNOS may be a therapeutic target.

show abstract

Inhibition of VEGF expression in tongue squamous cancer cells via RNA interference silencing of iNOS gene

Cited by 4 publications

References 11 publications

Applications of Nanosystems to Anticancer Drug Therapy (Part II. Dendrimers, Micelles, Lipid-based Nanosystems)

Applications of Nanosystems to Anticancer Drug Therapy (Part II. Dendrimers, Micelles, Lipid-based Nanosystems)

Colocalization of VEGF and iNOS in Parenchymal and Stromal Cells of Oral Squamous Cell Carcinoma

A study on the inhibition of VEGF expression in salivary gland adenoid cystic carcinoma cells via iNOS gene RNAi in vitro

Contact Info

Product

Resources

About