Inhibition of viral adsorption and penetration by an aqueous extract from Rhododendron ferrugineum L. as antiviral principle against herpes simplex virus type-1

Gescher, Kirsten; Kühn, Joachim; Hafezi, Wali; Louis, A.; Derksen, Andrea; Deters, Alexandra; Lorentzen, Eva; Hensel, Andreas

doi:10.1016/j.fitote.2010.11.022

Cited by 56 publications

(33 citation statements)

References 20 publications

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“…In contrast, among the tested phenolic compounds, only rosmarinic acid revealed some activity against attachment. Inhibition of adsorption and penetration of HSV by an aqueous extract from Rhododendron ferrugineum has been reported previously [34]. Similar results for inhibition of HSV attachment have been obtained with oligomeric proanthocyanidins from Rumex acetosa [33].…”

Section: Discussionsupporting

confidence: 84%

“…Antiherpetic activity has been shown for aqueous plant extracts from Echinacea pallidum [32], Rumex acetosa [33] and Rhododendron ferrugineum [34], for polysaccharides derived from marine algae [35], for steroidal compounds against adenovirus [36] and an ethylacetate-soluble fraction from the lichen Ramalina farinacea against HIV [37]. Although all analyzed drugs in our study demonstrated virucidal activity, Melissa extract demonstrated a higher virucidal activity when compared to single compounds.…”

Section: Discussionmentioning

confidence: 62%

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<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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Background: Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs for antiherpetic agents, since the herpes simplex virus (HSV) might develop resistance to commonly used antiviral drugs. Methods: An aqueous extract of Melissa officinalis as well as phenolic extract compounds, i.e. caffeic acid, p-coumaric acid and rosmarinic acid were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. Results: When drugs were added to HSV-1-infected cells, no antiviral effect was observed as determined by plaque reduction assay and analysis of expression of viral protein ICP0. However, the Melissa extract demonstrated a high virucidal activity against HSV-1, even at very low concentrations of 1.5 µg/ml, whereas similar results for phenolic compounds were only achieved at 100 times higher concentrations. Besides the virucidal activity, the Melissa extract and rosmarinic acid inhibited HSV-1 attachment to host cells in a dose-dependent manner. These results indicate that rosmarinic acid was the main contributor to the antiviral activity of Melissa extract. However, the selectivity index of Melissa extract of 875 against HSV is superior to the selectivity indices of single constituents. Conclusion: Melissa extract exhibits low toxicity, is virucidal and affects HSV-1 attachment to host cells in vitro.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 62%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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“…From these results it is assumed that the polysaccharide may interact with the immunological system. Some pre-and probiotics interact with the bacterial adhesion of pathogens to the eukaryotic binding receptors throughout so called antiadhesive compounds [29][30][31][32][33]. In case of pretreatment of AGS cells with EPS1190 the polysaccharide was shown to inhibit H. pylori adhesion by interaction with the surface of AGS cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells

Marcial¹,

Messing²,

Menchicchi³

et al. 2013

International Journal of Biological Macromolecules

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“…Nowadays, people pay close attention to Chinese herb medicines for combating diseases including antiviral activity. It has been reported that the extracts of Kumazasa, Solanum nigrum, Myrothamnus flabellifolia Welw and Alpinia katsumadai had anti-infection properties against Pseudorabies virus, hepatitis C virus, herpes simplex virus type 1 and rotaviruses, respectively (Gescher et al, 2011;Iwata et al, 2010;Javed et al, 2011;Kim et al, 2012), but these crude extracts from Chinese herb medicine possessed complex or undefined constituents which hampered the development of new antiviral drugs. In this experiment, we challenged the CEF cells with IBDV and observed the anti-IBDV activity of 20 constituents derived from Chinese herb medicines.…”

Section: Discussionmentioning

confidence: 99%

Antiviral effects of the constituents derived from Chinese herb medicines on infectious bursal disease virus

Sun

Song

Niu

et al. 2013

Pharmaceutical Biology

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Context: The prevalence of infectious bursal disease has brought about enormous financial losses to the world poultry industry. Chinese herb medicines can provide valuable materials for discovery and development of new drugs. Objective: To screen constituents derived from Chinese herb medicines for their antiviral activity against infectious bursal disease virus (IBDV) in vitro. Materials and methods: Twenty constituents derived from Chinese herb medicines and B87 strain of IBDV were used. The 50% cytotoxic concentration (CC 50 ) and 50% effective concentration (EC 50 ) were determined by visualization of cytopathologic effect (CPE) and 3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) test on chicken embryo fibroblast. Selectivity index (SI) and inhibition ratio (%I) were calculated from the data obtained from the MTT test. Results: Antiviral assays showed dipotassium glycyrrhizinate and ligustrazine hydrochloride among the 20 constituents tested exhibited significant inhibitory activity against IBDV in a dose-dependent manner. EC 50 of dipotassium glycyrrhizinate and ligustrazine hydrochloride were 663.2 AE 268.4 and 92.52 AE 21.13 mg/mL, and SI were 44.52 and 421.62, respectively. The time-of-addition and virucidal assay indicated that anti-IBDV activity of the two constituents could be due to their inhibiting virus replication and/or inactivating virus directly. The inhibition of virus attachment was not observed in the adsorption inhibition assay. Dipotassium glycyrrhizinate and ligustrazine hydrochloride exhibited more than 70% and 80% inhibition of IBDV, respectively, at the maximum safe concentration. Discussion and conclusion: We believe that dipotassium glycyrrhizinate and ligustrazine hydrochloride can be used to develop a new anti-IBDV compound, and it is worth applying the constituents in clinical practice.

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Inhibition of viral adsorption and penetration by an aqueous extract from Rhododendron ferrugineum L. as antiviral principle against herpes simplex virus type-1

Cited by 56 publications

References 20 publications

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells

Antiviral effects of the constituents derived from Chinese herb medicines on infectious bursal disease virus

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