Inhibition of virion-associated IPNV RNA polymerase, VP1, by radiolabeled nucleotide analogs

Villanueva, Rodrigo A.; Guacucano, Maritza; Pizarro, Javiera; Sandino, Ana María

doi:10.1016/j.virusres.2005.02.011

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…Due to the presence of positively-charged domains, it was speculated to interact with the inner plasmatic membrane leaflet [22]. VP1, the viral protein encoded by the genome segment B (with an approximate size of 94 kDa), is the viral RNA-dependent RNA-polymerase (RdRp) needed for genome replication and transcription [23]. As previously described, this structural protein is present in two forms: VP1, free inside the capsid, and VPg, linked to the 5 -ends of both genomes [4], probably only to the plus strands [17].…”

Section: The Viral Proteins and Their Functionmentioning

confidence: 99%

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

Dopazo

2020

Pathogens

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Infectious pancreatic necrosis (IPN) is a disease of great concern in aquaculture, mainly among salmonid farmers, since losses in salmonid fish—mostly very young rainbow trout (Salmo gairdnery) fry and Atlantic salmon (Salmo salar) post-smolt—frequently reach 80–90% of stocks. The virus causing the typical signs of the IPN disease in salmonids, named infectious pancreatic necrosis virus (IPNV), has also been isolated from other fish species either suffering related diseases (then named IPNV-like virus) or asymptomatic; the general term aquabirnavirus is used to encompass all these viruses. Aquabirnaviruses are non-enveloped, icosahedral bisegmented dsRNA viruses, whose genome codifies five viral proteins, three of which are structural, and one of them is an RNA-dependent RNA polymerase. Due to the great importance of the disease, there have been great efforts to find a way to predict the level of virulence of IPNV isolates. The viral genome and proteins have been the main focus of research. However, to date such a reliable magic marker has not been discovered. This review describes the processes followed for decades in the attempts to discover the viral determinants of virulence, and to help the reader understand how viral components can be involved in virulence modulation in vitro and in vivo. There is also a brief description of the disease, of host defenses, and of the molecular structure and function of the virus and its viral components.

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Section: The Viral Proteins and Their Functionmentioning

confidence: 99%

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

Dopazo

2020

Pathogens

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“…For the SEM study, one larva of D. silvatica was prepared following the technique of Ronderos et al (2000Ronderos et al ( , 2008. Ink illustrations were made using an attached camera lucida.…”

Section: Methodsmentioning

confidence: 99%

Morphology of the immature stages of Dasyhelea silvatica Wang, Zhang & Yu with redescriptions of adults (Diptera, Ceratopogonidae)

Li¹,

Duan²,

Yuan³

et al. 2020

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“…The specimens were mounted in Canada balsam following Yu et al (2005). For the Scanning Electron Microscope (SEM) study, one larva of D.alula was prepared following the technique of Ronderos et al (2000, 2008). Ink illustrations were made using an attached camera lucida.…”

Section: Methodsmentioning

confidence: 99%

First description of the immature stages of Dasyhelea alula and a redescription of adults from China (Diptera, Ceratopogonidae)

Duan

Jiang

Chang

et al. 2019

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The fourth instar larva and pupa of Dasyheleaalula Yu, 2005 are described and illustrated using a Scanning Electron Microscope. The adult male and female of this species are redescribed. Immatures were collected from flooded soil near a pond in Xiaojiawan village, Guizhou province, China and reared in the laboratory. The studied material is deposited in the Insect Collection of Zunyi Medical University.

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Inhibition of virion-associated IPNV RNA polymerase, VP1, by radiolabeled nucleotide analogs

Cited by 6 publications

References 22 publications

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

Morphology of the immature stages of Dasyhelea silvatica Wang, Zhang & Yu with redescriptions of adults (Diptera, Ceratopogonidae)

First description of the immature stages of Dasyhelea alula and a redescription of adults from China (Diptera, Ceratopogonidae)

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Inhibition of virion-associated IPNV RNA polymerase, VP1, by radiolabeled nucleotide analogs

Cited by 6 publications

References 22 publications

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

The Infectious Pancreatic Necrosis Virus (IPNV) and its Virulence Determinants: What is Known and What Should be Known

Morphology of the immature stages of Dasyhelea silvatica Wang, Zhang &amp; Yu with redescriptions of adults (Diptera, Ceratopogonidae)

First description of the immature stages of Dasyhelea alula and a redescription of adults from China (Diptera, Ceratopogonidae)

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Morphology of the immature stages of Dasyhelea silvatica Wang, Zhang & Yu with redescriptions of adults (Diptera, Ceratopogonidae)