Inhibition of α-glucosidase, pancreatic lipase, and antioxidant property of Myrcia hatschbachiiD. Legrand containing gallic and ellagic acids

Gatto, Larissa Junqueira; Oliveira, Gustavo Rezende Bellei de; Rech, Katlin Suellen; Moura, Paula Francislaine; Gribner, Caroline; Merino, Francis José Zortea; Ávila, Suélen; Dias, Josiane de Fátima Gaspari; Miguel, Obdúlio Gomes; Miguel, Marílis Dallarmi

doi:10.37360/blacpma.21.20.3.18

Cited by 7 publications

(5 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, long-term use of these drugs is often reported to cause some severe side effects, such as insomnia, headaches, hypoglycemia, weight gain, constipation, and renal damage [ 14 , 15 ]. Efforts have been directed toward discovering medicines from natural products due to their low costs, relative safety, probability of high compliance, and low incidences of undesirable side effects [ 16 ]. Based on previous studies, secondary metabolites of natural products, such as phenolics, alkaloids, terpenoids, flavonoids, and glycosides, have shown potent pancreatic lipase and α-glucosidase inhibitory activities [ 17 , 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of α-Glucosidase and Pancreatic Lipase Properties of Mitragyna speciosa (Korth.) Havil. (Kratom) Leaves

et al. 2022

View full text Add to dashboard Cite

Kratom (Mitragyna speciosa (Korth.) Havil.) has been used to reduce blood sugar and lipid profiles in traditional medicine, and mitragynine is a major constituent in kratom leaves. Previous data on the blood sugar and lipid-altering effects of kratom are limited. In this study, phytochemical analyses of mitragynine, 7-hydroxymitragynine, quercetin, and rutin were performed in kratom extracts. The effects on α-glucosidase and pancreatic lipase activities were investigated in kratom extracts and mitragynine. The LC-MS/MS analysis showed that the mitragynine, quercetin, and rutin contents from kratom extracts were different. The ethanol extract exhibited the highest total phenolic content (TPC), total flavonoid content (TFC), and total alkaloid content (TAC). Additionally, compared to methanol and aqueous extracts, the ethanol extract showed the strongest inhibition activity against α-glucosidase and pancreatic lipase. Compared with the anti-diabetic agent acarbose, mitragynine showed the most potent α-glucosidase inhibition, with less potent activity of pancreatic lipase inhibition. Analysis of α-glucosidase and pancreatic lipase kinetics revealed that mitragynine inhibited noncompetitive and competitive effects, respectively. Combining mitragynine with acarbose resulted in a synergistic interaction with α-glucosidase inhibition. These results have established the potential of mitragynine from kratom as a herbal supplement for the treatment and prevention of diabetes mellitus.

show abstract

Section: Introductionmentioning

confidence: 99%

Inhibition of α-Glucosidase and Pancreatic Lipase Properties of Mitragyna speciosa (Korth.) Havil. (Kratom) Leaves

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In view of the advantages of good antibacterial potency, antioxidant property and biocompatibility [ 32 ], gallic acid was chosen as a reducing agent for silver nanoparticle synthesis from silver ions. GA–Ag NPs are spherical in shape, with good stability and polydispersity ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Collagen-based injectable and self-healing hydrogel with multifunction for regenerative repairment of infected wounds

Han

Wei

et al. 2023

Regenerative Biomaterials

View full text Add to dashboard Cite

At present, the development trend of dressing materials is being multifunctional for convenient and long-term nursing care process of some complicated wounds. Here, basing on the theory of wound moist healing, an injectable and self-healing hydrogel comprising of collagen (COL), chitosan (CS) and OKGM, which acts as a macromolecular cross-linker to construct dynamic Schiff -base bonds was smartly designed. The strategy of introducing the Ag nanoparticles (Ag NPs) into the COL-CS-OKGM hydrogel matrix achieved a markedly enhanced antibacterial activity derived from the synergistical effect between the Ag+ and the mild photothermal efficacy of Ag NPs, which also improved the local capillary blood circulation of the wound area to further facilitate wound healing process. The excellent syringeability and self-healing behaviors endowed the COL-CS-OKGM-Ag hydrogel with self-adapting ability for the wounds with irregular and large area needing frequent applying and changing without secondary injuries. In vitro and in vivo evaluations verified that so-designed COL-CS-OKGM-Ag hydrogel also with haemostatic performance is a promising multifunctional dressing for the treatments of infected wound with not only good biocompatibility and convenient use, but also with desired regenerative healing prognoses benefited from hydrogel moist environment and physiotherapy.

show abstract

“…In order to verify the specificity and feasibility of the synthetic immobilized PPL, a standard mixture model consisting of hesperidin (lipase inhibitor, IC 50 = 64.92 µM, as positive control) and gallic acid (no lipase inhibitory activity, as negative control) was designed for validation [ 24 , 39 ]. The HPLC analysis results of each eluent are shown in Figure 7 , where S1 is the chromatogram of the model mixture, and S2 is the chromatogram of the methanol elution of the MMOF after ligand fishing.…”

Section: Resultsmentioning

confidence: 99%

Rapid Screening of Lipase Inhibitors in Scutellaria baicalensis by Using Porcine Pancreatic Lipase Immobilized on Magnetic Core–Shell Metal–Organic Frameworks

Cao

Fan

et al. 2022

Molecules

View full text Add to dashboard Cite

As for ligand fishing, the current immobilization approaches have some potential drawbacks such as the small protein loading capacity and difficult recycle process. The core–shell metal–organic frameworks composite (Fe3O4-COOH@UiO-66-NH2), which exhibited both magnetic characteristics and large specific surface area, was herein fabricated and used as magnetic support for the covalent immobilization of porcine pancreatic lipase (PPL). The resultant composite Fe3O4-COOH@UiO-66-NH2@PPL manifested a high loading capacity (247.8 mg/g) and relative activity recovery (101.5%). In addition, PPL exhibited enhanced tolerance to temperature and pH after immobilization. Then, the composite Fe3O4-COOH@UiO-66-NH2@PPL was incubated with the extract of Scutellaria baicalensis to fish out the ligands. Eight lipase inhibitors were obtained and identified by UPLC-Q-TOF-MS/MS. The feasibility of the method was further confirmed through an in vitro inhibitory assay and molecular docking. The proposed ligand fishing technique based on Fe3O4-COOH@UiO-66-NH2@PPL provided a feasible, selective, and effective platform for discovering enzyme inhibitors from natural products.

show abstract

Inhibition of α-glucosidase, pancreatic lipase, and antioxidant property of Myrcia hatschbachiiD. Legrand containing gallic and ellagic acids

Cited by 7 publications

References 56 publications

Inhibition of α-Glucosidase and Pancreatic Lipase Properties of Mitragyna speciosa (Korth.) Havil. (Kratom) Leaves

Inhibition of α-Glucosidase and Pancreatic Lipase Properties of Mitragyna speciosa (Korth.) Havil. (Kratom) Leaves

Collagen-based injectable and self-healing hydrogel with multifunction for regenerative repairment of infected wounds

Rapid Screening of Lipase Inhibitors in Scutellaria baicalensis by Using Porcine Pancreatic Lipase Immobilized on Magnetic Core–Shell Metal–Organic Frameworks

Contact Info

Product

Resources

About