Inhibitors of the adhesiveness of enteropathogenicE. coli

Abstract

The entero-pathogenic strain of E. coli 0125: K 70 was able to adhere to washed guinea-pig erythrocytes and to cause their agglutination. Electron microscopy revealed this strain to be rich in fimbriae. They were able to cause hemagglutination by themselves, generally at protein concentration of 10 to 100 mug per ml. D-mannose and alpha-methylmannoside were able to inhibit hemagglutination by the whole bacteria or their isolated fimbriae at concentrations of 0.003 to 0.012 mg/ml. L-mannose, D-lactose, D-glucose, N-acetylglucosamine, D-galactose, N-acetyl-D-galactosamine, D-fucose, did not exert any inhibiting effect at concentrations as high as 50 mg/ml. N-acetyl-neuraminic acid was also uneffective at concentration as high as 9 mg/ml.

Membrane cofactor protein (MCP or CD46) is a cellular pilus receptor for pathogenic Neisseria

“…A number of bacterial pili have been established as having lectin-like properties, including type IV pili of P. aeruginosa, which bind to ␤GalNAc(1-4)␤Gal of glycosphingolipids Sheth et al, 1994), the type 1 fimbriae of Escherichia coli, which bind to mannose residues (Salit and Gotschlich, 1977;Rivier and Darekar, 1975), and the E. coli pap-pili with binding specificity to ␣Gal(1-4)␤ disaccharides (Bock et al, 1985). We have previously investigated the nature of the gonococcal host cell receptor and found that the pilus-mediated attachment of the bacteria to human tissue is mediated by a eukaryotic receptor with protein characteristics (Jonsson et al, 1994).…”

Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans

“…This reaction may be termed mannose-resistant hemagglutination (MR-HA). In contrast, the HA reaction mediated by another kind of pili, termed type 1 pili, is inhibited by mannose (6,30). Type 1 pili are present on a wide variety of pathogenic and nonpathogenic E. coli strains (2-4) and therefore are not thought to be correlated with the ability to cause human diarrheal disease.…”

Genetic analysis of complex gene clusters inEscherichia coli: the genetic analysis of F72 fimbrial genes

“…The presence of mannose or mannose-like compounds will therefore inhibit the HA. In such cases one refers to mannosesensitive haemagglutination (MSHA) (Brinton, 1965;Rivier and Darekar, 1975;Satit and Gotschlich, 1977). In other cases, e.g.…”