Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans

Deneke, Carl F.; Thorne, Grace M.; Gorbach, Sherwood L.

doi:10.1128/iai.26.1.362-368.1979

Cited by 47 publications

(18 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All P fimbriae yielded two bands in the SDS-PAGE; one of the preparations (KS71) even yielded three bands. A similar multiple SDS-PAGE pattern of purified fimbrial preparations has recently been reported from an E. coli strain causing diarrhea (3), as well as from other strains (10). We do not consider the various bands to be nonfimbrial contaminants since different batches of purified fimbriae gave identical SDS-PAGE patterns.…”

Section: Discussionsupporting

confidence: 58%

P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains

Korhonen¹,

Väisänen²,

Saxén³

et al. 1982

Infect Immun

151

View full text Add to dashboard Cite

P-antigen-recognizing fimbriae (P fimbriae) from four pyelonephritogenic Escherichia coli strains and type 1 fimbriae from an E. coli strain and a Salmonella typhimurium strain were purified. The P fimbriae were morphologically similar to type 1 fimbriae. The purified P fimbriae agglutinated neuraminidase-treated human P1 and P2k erythrocytes but not p erythrocytes, which lack all P-blood group-specific glycosphingolipids. However, coating of neuraminidase-treated p erythrocytes with globoside rendered such erythrocytes agglutinable by the P fimbriae. The hemagglutinations were in all instances fully inhibited by the synthetic alpha-D-Galp-(1-4)-beta-D-Galp-1-O-Me glycoside. The binding specificity of the P fimbriae could also be demonstrated by using fimbriae coated onto latex particles and nontreated erythrocytes. It was thus concluded that the P fimbriae recognize and bind to the alpha-D-Galp-(1-4)-beta-D-Galp carbohydrate sequence occurring in the series of P-blood group antigen-specific glycosphingolipids. In contrast to both type 1 fimbriae, all four P fimbriae preparations showed multiple bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antisera were raised in rabbits against the various E. coli fimbriae. In enzyme-linked immunosorbent assays each one of the antisera to the P fimbriae reacted to titers of log 4 to 7 with both the homologous and the heterologous P fimbriae, but not with the type 1 fimbriae of E. coli. In a reciprocal fashion, the antiserum to the type 1 fimbriae of one E. coli strain reacted only with the homologous type 1 but not with any of the P fimbriae preparations.

show abstract

Section: Discussionsupporting

confidence: 58%

P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains

Korhonen¹,

Väisänen²,

Saxén³

et al. 1982

Infect Immun

151

View full text Add to dashboard Cite

show abstract

“…Recently Deneke et al (6,7) have identified three distinct pilus serotypes found on ETEC strains. Antisera raised against these pili reacted with 56% of ETEC strains from humans.…”

Section: Discussionmentioning

confidence: 99%

New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans

Thomas¹,

Cravioto²,

Scotland³

et al. 1982

Infect Immun

115

View full text Add to dashboard Cite

An enterotoxigenic strain of Escherichia coli 025:H42 (strain E8775), isolated from a patient in Bangladesh with diarrhea, caused mannose-resistant hemagglutination (MRHA) of human and bovine erythrocytes. The strain did not show slide agglutination or immunodiffusion precipitin lines with antiserum specific for the colonization factor antigen CFAII or CFAIII. A variant E. coli strain, E8775-B, did not cause MRHA or produce enterotoxin. Electron microscopy revealed the presence of fimbriae on the surface of strain E8775 but not strain E8775-B. When strain E8775 was grown at 22°C, it became MRHA negative and fimbriae were absent. An antiserum prepared against strain E8775 was absorbed with strain E8775-B to make an antiserum specific for the fimbrial antigen. Using this absorbed antiserum, we found the fimbrial antigen in 48 of 742 enterotoxigenic E. coli strains. The 48 strains belonged to serogroups 025, 0115, and 0167. It is suggested by analogy to the properties of previously described colonization factors that these fimbriae may play a part in the colonization of the intestinal epithelium.

show abstract

“…The fractionation method described was a modification of the procedures described by Nowicki et al [19] and Deneke et al [22]. An S-fimbriated population was obtained by adding Saccharomyces cerevisiae cells [23] (final concentration 10% (v/v)) to 2 ml of bacterial culture (10 '1 cells/ml).…”

Section: Fractionation Of Cellsmentioning

confidence: 99%

Fractionation of a bacterial cell population by adsorption to erythrocytes and yeast cells

Nowicki

1985

FEMS Microbiology Letters

View full text Add to dashboard Cite

Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans

Cited by 47 publications

References 37 publications

P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains

P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains

New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans

Fractionation of a bacterial cell population by adsorption to erythrocytes and yeast cells

Contact Info

Product

Resources

About