New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans

Abstract

An enterotoxigenic strain of Escherichia coli 025:H42 (strain E8775), isolated from a patient in Bangladesh with diarrhea, caused mannose-resistant hemagglutination (MRHA) of human and bovine erythrocytes. The strain did not show slide agglutination or immunodiffusion precipitin lines with antiserum specific for the colonization factor antigen CFAII or CFAIII. A variant E. coli strain, E8775-B, did not cause MRHA or produce enterotoxin. Electron microscopy revealed the presence of fimbriae on the surface of strain E8775 but not strain E8775-B. When strain E8775 was grown at 22°C, it became MRHA negative and fimbriae were absent. An antiserum prepared against strain E8775 was absorbed with strain E8775-B to make an antiserum specific for the fimbrial antigen. Using this absorbed antiserum, we found the fimbrial antigen in 48 of 742 enterotoxigenic E. coli strains. The 48 strains belonged to serogroups 025, 0115, and 0167. It is suggested by analogy to the properties of previously described colonization factors that these fimbriae may play a part in the colonization of the intestinal epithelium.

Haemagglutination Patterns of Aeromonas Spp. Related to Species and Source of Strains

“…Adherence of £". coli to intestinal mucosa mediated by colonisation factors with recognised patterns of haemagglutination (Evans, Evans and DuPont, 1979b) or other adhesins (Thomas et aL, 1982;Honda et al, 1983) precedes the development of diarrhoea caused by enterotoxigenic E. coli (Satterwhite et aL, 1978). For Aeromonas spp., mechanisms of pathogenesis are not defined, although analogy with known enteric pathogens suggests these bacteria may produce several possible virulence factors (Burke and Gracey, 1986).…”

“…Colonisation factors (CFA) of E. coli have been studied in detail. At least four CFA's have been described (Evans et al, 1975;Evans and Evans, 1978;Thomas et al, 1982;Honda, Arita and Miwatani, 1984) and two of these, CE\I and CE\II, have been well characterised (Evans et al, 1975;. Both CE^I and CFAII were first recognised on the basis of patterns of haemagglutination of different species of red cells in the presence or absence of sugars.…”

Cloning of the Genes That Control Formation of the Fimbrial Colonization Factor Antigen III (CFA/III) from an Enterotoxigenic Escherichia coli

“…Although the genetic determinants of several colonization factors of ETEC from human, namely, CFA/I (5,8,13), CFA/II (2, 4, 17,19), CFA/IV (3, 23), and PCF09 (9) have been cloned, the results of genetic studies on them are still premature. McConnell and Rowe (18) have found a plasmid which is responsible for formation of CFA/III pili, but genetic analysis of the plasmid has not been done.…”

Comparison between Enterotoxigenic Escherichia coli Strains Expressing “F42,” F41 and K99 Colonization Factors

“…Several colonization factors (CFs) have been reported so far as important for human ETEC strains adherence: CFA/I (12), CFA/II (13), CFA/III (7), PCF8775 (37) and PCFO159 (22). Among animal ETEC strains, antigens K88 (31), 987P (28), K99 (32) and F41 (8) have been described.…”