Inhibitors of the LPS-induced NF-?B activation from

Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators

“…This assay nonetheless gave a clear indication of toxicity against HeLa cells through indications of cell shrinkage or cells floating following exposure to the extracts (carrier solvent controls did not show any of these indicators of toxicity). The active species from this set, for which previous studies exist, include Turkish (Yesilada et al, 1997;Yesilada and Kupeli, 2007), East Asian (Suh et al, 2006;Park et al, 2006a) and Australian species of Clematis ; species of Artemisia from China (Jin et al, 2004;Lee et al, 2007) and Tibet (Sun et al, 2006). As identified in Jin et al (2004) sesquiterpene lactones are the likely NF-B inhibitors found in extracts of Artemisia spp.…”

“…The active species from this set, for which previous studies exist, include Turkish (Yesilada et al, 1997;Yesilada and Kupeli, 2007), East Asian (Suh et al, 2006;Park et al, 2006a) and Australian species of Clematis ; species of Artemisia from China (Jin et al, 2004;Lee et al, 2007) and Tibet (Sun et al, 2006). As identified in Jin et al (2004) sesquiterpene lactones are the likely NF-B inhibitors found in extracts of Artemisia spp. Hehner et al, 1998Hehner et al, , 1999Jain and Kulkarni, 1999;Kwok et al, 2001;Smolinski and Pestka, 2003;Sun et al, 2007).…”

The Artemisia L. Genus

“…Some of these compounds were isolated by bioassay-guided fractionation, after previously detecting activity on the part of the plant. Three new guaianolide sesquiterpene lactones, together with six known sesquiterpenes, were isolated by bioassay-guided fractionation from the methanol extract of the aerial parts of A. sylvatica [25]. From A. anomala, Wen et al [26] isolated a new dimeric guaianolide, Fig.…”

“…This compound, artemisolide, together with several sesquiterpene lactones such as 3α,4α-epoxyrupicolins C-E, 3-methoxytanapartholide, deacetyllaurebiolide, moxartenolide and arteminolides B and D, Fig. 3, were also isolated by bioassay-guided fractionation from the methanol extract of the aerial parts of A. sylvatica, using the NF-κB mediated reporter gene assay [25]. All isolated compounds displayed inhibitory activity on the LPS-induced NF-κB activation, and NO and TNF-α production.…”

Therapeutic Potential of Natural Products from Terrestrial Plants as TNF-α Antagonist

“…All isolated compounds displayed inhibitory activity on the LPS-induced NF-B activation, NO production, and TNF-production with IC 50 values of 0.49-7.17, 1.46-6.16, and 3.19-27.76 M, respectively, in RAW264.7 cells [33].…”