Inhibitory action of hydrogen sulfide on muscarinic receptor-induced contraction of isolated porcine irides

Monjok, Emmanuel; Kulkarni, Kaustubh H.; Kouamou, Ghislaine; McKoy, Marshalyn G.; Opere, Catherine A.; Bongmba, Odelia N.; Njie, Ya Fatou; Ohia, Sunny E.

doi:10.1016/j.exer.2008.09.011

Cited by 37 publications

(36 citation statements)

References 30 publications

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“…Data from these studies also show that NaHS was more potent than Na 2 S in stimulating cyclic AMP production in both bovine and porcine retinae. A similar change in sensitivity of tissues to NaHS and Na 2 S has been reported by our laboratory in studies of sympathetic neurotransmission in porcine iris-ciliary body [19], amino acid transmission in bovine retina [20] and in the relaxation of isolated porcine irides to both H 2 S donors [18]. In the present study, we also observed that the ability of H 2 S donors to increase cyclic AMP concentrations was greater in bovine than porcine retina, suggesting that species differences exist in the response of this tissue to H 2 S donors.…”

Section: Discussionsupporting

confidence: 64%

“…Furthermore, studies have reported that deficiency of CBS is often associated with many eye disorders including retinal degeneration, retinal detachment, optical atrophy and glaucoma [16,17]. Evidence from our laboratory demonstrate that H 2 S (using sodium hydrosulfide, NaHS and sodium sulfide, Na 2 S as donors) can induce pharmacological effects in mammalian ocular tissues [18][19][20]. We found that both NaHS and Na 2 S relaxed pre-contracted isolated porcine irides [18] and inhibited sympathetic and glutamatergic neurotransmission from isolated porcine and bovine anterior uvea and retina, an effect that was shown to be dependent on intramural biosynthesis of this gas [19,20].…”

Section: Introductionmentioning

confidence: 88%

“…Evidence from our laboratory demonstrate that H 2 S (using sodium hydrosulfide, NaHS and sodium sulfide, Na 2 S as donors) can induce pharmacological effects in mammalian ocular tissues [18][19][20]. We found that both NaHS and Na 2 S relaxed pre-contracted isolated porcine irides [18] and inhibited sympathetic and glutamatergic neurotransmission from isolated porcine and bovine anterior uvea and retina, an effect that was shown to be dependent on intramural biosynthesis of this gas [19,20]. In the cardiovascular system, the pharmacological effects of H 2 S has been reported to be mediated largely by ATP-sensitive potassium channels (K ATP ) [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Hydrogen Sulfide on Cyclic AMP Production in Isolated Bovine and Porcine Neural Retinae

et al. 2009

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Hydrogen sulfide (H(2)S) has been reported to exert pharmacological effects on neural and non-neural tissues from several mammalian species. In the present study, we examined the role of the intracellular messenger, cyclic AMP in retinal response to H(2)S donors, sodium hydrosulfide (NaHS) and sodium sulfide (Na(2)S) in cows and pigs. Isolated bovine and porcine neural retinae were incubated in oxygenated Krebs buffer solution prior to exposure to varying concentrations of NaHS, Na(2)S or the diterpene activator of adenylate cyclase, forskolin. After incubation at different time intervals, tissue homogenates were prepared for cyclic AMP assay using a well established methodology. In isolated bovine and porcine retinae, the combination of both phosphodiesterase inhibitor, IBMX (2 mM) and forskolin (10 microM) produced a synergistic increase (P < 0.001) in cyclic AMP concentrations over basal levels. NaHS (10 nM-100 microM) produced a time-dependent increase in cyclic AMP concentrations over basal levels which reached a maximum at 20 min in both bovine and porcine retinae. At this time point, both NaHS and Na(2)S (10 nM-100 microM) caused a significant (P < 0.05) dose-dependent increase in cyclic AMP levels in bovine and porcine retinae. For instance, NaHS (100 nM) elicited a four-fold and three-fold increase in cyclic AMP concentrations in bovine and porcine retinae respectively whilst higher concentrations of Na(2)S (100 microM) produced a much lesser effect in both species. In bovine and porcine retinae, the effects caused by forskolin (10 microM) on cyclic AMP production were not potentiated by addition of low or high concentrations of both NaHS and Na(2)S. We conclude that H(2)S donors can increase cyclic AMP production in isolated neural retinae from cows and pigs. Bovine retina appears to be more sensitive to the stimulatory effect of H(2)S donors on cyclic nucleotide production than its porcine counterpart indicating that species differences exist in the magnitude of this response. Furthermore, effects produced by forskolin on cyclic AMP formation were not additive with those elicited by H(2)S donors suggesting that these agents may share a common mechanism in their action on the adenylyl cyclase pathway.

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Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Effect of Hydrogen Sulfide on Cyclic AMP Production in Isolated Bovine and Porcine Neural Retinae

et al. 2009

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“…10 At postjunctional sites, H 2 S donors and its substrate, lcysteine, have also been reported relax precontracted isolated porcine irides, an effect that was partially dependent upon endogenous biosynthesis of H 2 S in this tissue. 11,12 Furthermore, in bovine posterior ciliary arteries, H 2 S donors such as NaHS, GYY 4137, AP67, and AP72 have been shown to relax phenylephrine-induced tone by an action that is partially dependent upon the endogenous production of H 2 S, prostanoids, and K ATP channels. 13,14 In the retina, H 2 S donors can inhibit excitatory amino acid neurotransmission from both isolated bovine and porcine tissues by an effect that was partially dependent on the endogenous biosynthesis of this gas.…”

Section: Introductionmentioning

confidence: 99%

Effect of Hydrogen Sulfide Donors on Intraocular Pressure in Rabbits

Salvi

Bankhele

Jamil

et al. 2016

Journal of Ocular Pharmacology and Therapeutics

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Purpose: In this study, we investigated the effect of a slow-releasing hydrogen sulfide (H 2 S) donor, GYY 4137, on intraocular pressure (IOP) in normotensive rabbits. Furthermore, we compared the IOP-lowering action of GYY 4137 with those elicited by other H 2 S-producing compounds, l-cysteine and ACS67 (a hybrid compound of latanoprost with an H 2 S-releasing moiety). Methods: IOP was measured in New Zealand normotensive male albino rabbits using a pneumatonometer (model 30 classic; Reichert Ophthalmic Instruments, Depew, NY). At 0 h, 50 mL of test compounds were applied topically to 1 eye of each animal, while the contralateral eye received the same quantity of vehicle (saline). IOP was measured hourly until baseline IOP readings were attained and animal eyes monitored for potential side effects (i.e., tearing, hyperemia). Results: GYY 4137 (0.1%-2%) produced a dose-dependent decrease in IOP reaching a maximum of 27.8% -3.14% (n = 5) after 6 h. Interestingly, a significant contralateral effect was observed in vehicle-treated controls eyes at all doses tested. l-cysteine (5%) and ACS67 (0.005%) also elicited a significant (P < 0.01) decrease in IOP that achieved a maximum of 28.84% -1.53% (n = 5) and 23.27% -0.51% (n = 5), respectively, after 3 h. All 3 H 2 S-producing compounds also caused a significant contralateral effect in vehicle-treated control eyes. Conclusion: We conclude that GYY 4137 and other H 2 S-producing donors can reduce IOP in normotensive rabbits. However, the profile of IOP-lowering action of GYY 4137 was different from the other H 2 S donors affirming its ability to act as a slow-releasing gas donor.

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“…Констрикція цих м'язів, викли ка на кар-бохолом, знімалася при дії донора сірковод-ню. Автори дійшли висновку, що інгібуюча дія цих речовин в ізольованих райдужках свині залежить від біосинтезу сірководню за участю ферментів цистеїн-γ-ліази і цистеїн-β-синтази [16]. В очах кроликів з нормотензією було показано вплив двох донорів сірководню GYY4137 і ACS67 в зниженні симпатичної нейропередачі в судинній мережі очей [17].…”

Section: результати та їх обговоренняunclassified

Effect of Hydrogen Sulphide Donor on Intraocular Pressure in Rats

Mikheytseva¹,

Siroshtanenko²

2016

Fiziol Zh

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Досліджували вплив 1%-го розчину гідросульфіду натрію (NaHS) на внутрішньоочний тиск (ВОТ)уНормальний внутрішньоочний тиск (ВОТ) -важлива складова гомеостазу. Під-тримка йо го рів ня забезпечується складним механізмом регуляції руху внутрішньоочної рідини. Порушення цього процесу є патоге-нетичним фактором розвитку таких очних захворювань, як глаукома. У механізмах регуляції ВОТ задіяні структури циліар но го тіла і трабекулярної мережі. Гідроди наміка очей здійснюється за участю гладень ко-м'я зових клітин цих структур. Тому дово-лі ймовірним регулятором цих процесів може бути молекула сірководню. Однак на сьогоднішній день щодо участі H 2 S у регуляції функцій очей літературних даних дуже мало. Існують окремі відомості, що він може викликати фармакологічні ефекти в тканинах очей ссавців, змінювати симпатич ну і глутаматергічну нейротрансмісію, відігравати регуляторну роль у процесах сигнальної трансдукції в очах [7]. Є повідомлення про наявність ферментів, які здійснюють біосин-тез сірководню в очних тканинах [8,9]. На відміну від вивчення значення і впливу сірководню в нервовій і серцево-судинній системі, практично відсут ні дослідження, спрямовані на встановлення фармакологічного ефекту цієї речовини на ВОТ, його потенційних можливостей у впливі на вивільнення і/або доступність нейротрансмітерів у передньому відділі ока, а також прямої дії сірководню на гладеньком'язові структури очей. Виявлен ня регуляторної функції сірководню в оці, впли ву

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Inhibitory action of hydrogen sulfide on muscarinic receptor-induced contraction of isolated porcine irides

Cited by 37 publications

References 30 publications

Effect of Hydrogen Sulfide on Cyclic AMP Production in Isolated Bovine and Porcine Neural Retinae

Effect of Hydrogen Sulfide on Cyclic AMP Production in Isolated Bovine and Porcine Neural Retinae

Effect of Hydrogen Sulfide Donors on Intraocular Pressure in Rabbits

Effect of Hydrogen Sulphide Donor on Intraocular Pressure in Rats

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