Inhibitory action of polyunsaturated fatty acids on IMP dehydrogenase

Mizushina, Yoshiyuki; Dairaku, Issei; Yanaka, Noriyuki; Takeuchi, Toshifumi; Ishimaru, Chisato; Sugawara, Fumio; Yoshida, Hiromi; Katô, Norihisa

doi:10.1016/j.biochi.2007.01.009

Cited by 38 publications

(22 citation statements)

References 21 publications

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“…1) This enzyme is a critical determinant of the level of guanine nucleotides, including guanosine triphosphate (GTP), that are essential for many cellular functions.…”

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confidence: 99%

“…1) The assay procedure for IMPDH was described in the same report, 1) in which various polyphenols were added to the assay mixture by dissolving in 10% dimethyl sulfoxide at 1 mM. Colon carcinoma cell line HT-29 cells were maintained in Dulbecco's modified Eagle's medium (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal calf serum, 100 units/ml of penicillin, and 100 mg/ml of streptomycin at 37 C in 5% CO 2 .…”

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confidence: 99%

“…9) Increased IMPDH activity is correlated with increased cellular proliferation and transformation. 1) In our MTT assay, the relative values of cell growth (%) at 0, 50, 75, and 100 mM of curcumin (n ¼ 5) were 100 AE 1, 31:6 AE 0:3, 26:2 AE 0:4, and 17:0 AE 0:2, respectively (curcumin effect at 50-100 mM, p < 0:05). These results indicate that 50 mM curcumin had no effect on cellular GTP, but significantly suppressed the cell growth.…”

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confidence: 99%

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Inhibitory Effect of Curcumin on IMP Dehydrogenase, the Target for Anticancer and Antiviral Chemotherapy Agents

Dairaku

Han

Yanaka

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…1) This enzyme is a critical determinant of the level of guanine nucleotides, including guanosine triphosphate (GTP), that are essential for many cellular functions.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Inhibitory Effect of Curcumin on IMP Dehydrogenase, the Target for Anticancer and Antiviral Chemotherapy Agents

Dairaku

Han

Yanaka

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…According to molecular dynamics simulations, DHA exhibits high conformational flexibility and undergoes rapid interconversion between torsional states, including extended and bent conformation [46]. According to computational analyses of fatty acids containing 20 and 22 carbons, DHA, AA, and EPA form ball-shaped curves [47,48]. DHA and AA have six and four double bonds, respectively, in their carbon skeleton, and these double bonds may face each other.…”

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confidence: 99%

Evaluation of the inhibitory effect of docosahexaenoic acid and arachidonic acid on the initial stage of amyloid β1-42 polymerization by fluorescence correlation spectroscopy

Miwa¹,

Hashimoto²,

Hossain³

et al. 2013

AAD

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Amyloid β (Aβ) 1-42 fibrillation is a crucial step in the development of pathological hallmarks, such as neuritic plaques and neurofibrillary tangles, of Alzheimer's disease (AD). In this study, we evaluated the effects of free docosahexaenoic acid (DHA), an essential brain polyunsaturated fatty acid (PUFA), on the inhibition of Aβ 1-42 fibrillation by fluorescence correlation spectroscopy (FCS), a technique capable of detecting molecular movements and interactions in solution. We also examined whether free arachidonic acid (AA), eicosapentaenoic acid (EPA), and metabolites of DHA, including neuroprotectin D1 (NPD1, 10S, 17S-dihydroxy-DHA), resolvin D1 (RvD1, 7S, 8R, 17S-trihydroxy-DHA), and didocosahexaenoyl glycerol (diDHA), affect Aβ 1-42 polymerization. The results of the FCS study reveal that DHA and AA significantly reduced the diffusion time of TAMRA (5-carboxytetramethylrhodamine)-Aβ 1-42 by 28% and 31%, respectively, while EPA, NPD1, RvD1, and diDHA had no effects on diffusion time. These results indicate that DHA and AA inhibited Aβ 1-42 polymerization and that their inhibitory effects occurred at the initial stage of Aβ 1-42 polymerization. This study will advance the research on PUFAs in preventing AD progression.

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“…The in vitro activities of DNA polymerase (8-10), DNA topoisomerase II (11), IMP dehydrogenase (12), polynucleotide kinase (8) and deoxyribonuclease (8) of T. rubrum mycelia extract were assayed at 37˚C as described previously (8)(9)(10)(11)(12). The enzymes were used at 0.05 units each.…”

Section: Antifungal (Sterilization Of T Rubrum) Assaymentioning

confidence: 99%

Antifungal properties of Japanese cedar essential oil from waste wood chips made from used sake barrels

Takao¹,

Kuriyama

Yamada³

et al. 2012

Mol Med Report

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Abstract. In this study, we prepared essential oil (EO) from waste wood chips made from used sake barrels (USBs) of Japanese cedar (i.e., EO-USB) by steam distillation. We found that EO-USB and three commercially purchased EOs derived from xylem tissue of Japanese woods, such as Japanese cedar (Cryptomeria japonica), Japanese cypress (Chamaecyparis obtusa) and false arborvitae (Thujopsis dolabrata), suppressed fungal growth activity against Trichophyton rubrum, which is the cause of tinea disease. The magnitude of the suppressive effects of the EOs ranked as follows: T. dolabrata > USB = C. japonica > C. obtusa. These EOs also inhibited the activity of DNA polymerase in an extract from T. rubrum mycelia with the following ranking: T. dolabrata > USB = C. japonica > C. obtusa. In addition, 50 µg/ml of EO-USB showed antifungal properties, killing T. rubrum mycelia at 27-42˚C in 20 min. By gas chromatography/mass spectrometry analysis, the main sesquiterpenes in EO-USB were δ-cadinene (25.94%) and epi-cubenol (11.55%), and the composition of EO-USB was approximately the same as that of EO-C. japonica. Three prepared sesquiterpenes, δ-cadinene, epi-cubenol and β-eudesmol, inhibited the fungal growth and DNA polymerase activities of T. rubrum, and epi-cubenol showed the strongest inhibition among the compounds tested. These sesquiterpenes had no inhibitory effects on the activities of other DNA metabolic enzymes, such as DNA topoisomerase II, IMP dehydrogenase, polynucleotide kinase and deoxyribonuclease from T. rubrum. Taken together, these results suggest that EO-USB containing epi-cubenol may be useful for its anti-tinea disease properties, which are based on DNA polymerase inhibition.

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Inhibitory action of polyunsaturated fatty acids on IMP dehydrogenase

Cited by 38 publications

References 21 publications

Inhibitory Effect of Curcumin on IMP Dehydrogenase, the Target for Anticancer and Antiviral Chemotherapy Agents

Inhibitory Effect of Curcumin on IMP Dehydrogenase, the Target for Anticancer and Antiviral Chemotherapy Agents

Evaluation of the inhibitory effect of docosahexaenoic acid and arachidonic acid on the initial stage of amyloid β1-42 polymerization by fluorescence correlation spectroscopy

Antifungal properties of Japanese cedar essential oil from waste wood chips made from used sake barrels

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