Inhibitory actions of genistein in human breast cancer (MCF-7) cells

Chen, Wenfang; Huang, Ming‐Hui; Tzang, Chi-Hung; Yang, Mengsu; Wong, Man Sau

doi:10.1016/s0925-4439(03)00082-6

Cited by 110 publications

(84 citation statements)

References 29 publications

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“…The mRNA expression levels were normalized to the expression of a housekeeping gene GAPDH. SYBR-Green-I fluorescence labeling method was used in real-time quantitative RT-PCR amplification on an ABI Prism 7000 SDS and analyzed with its software according to the standard protocol and manufacturer's instructions [20] . The 2 -ΔΔCT method was used in data analysis based on similar (<10%) amplification efficiency of the target and reference genes, which was achieved by the determination of ΔCT variations with template dilution [21] .…”

Section: R : T G a T G T G G A G G T T C A G C A C C 1 9 7 B P ) A mentioning

confidence: 99%

Anti-hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana

Tian

Geng²,

Zheng³

et al. 2006

WJG

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hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana . World J Gastroenterol 2006; 12(6): 874-879 http://www.wjgnet.com/1007-9327/12/874.asp INTRODUCTIONHepatocellular carcinoma is one of the most common malignant neoplasms worldwide and one of the leading causes of malignancy-related death in China [1,2] . Its therapy in clinic is still a big challenge.Ursolic acid (UA) is a versatile compound, which possesses anti-cancer [3,4] , anti-inflammatory [5,6] , anti-HIV [7] and immunomodulatory effects [8] . There is a growing interest in the anti-tumor activities of UA. UA could act on almost all steps in the whole cancer process: initiation, promotion, progression, and metastasis. Multiple lines of evidence indicate that UA is a promising chemo-preventive agent both in vivo and in vitro. UA could inhibit Epstein-Barr virus activation induced by 12-O-tetradecanoylphorbol-13-acetate in Raji cells and two-stage mouse skin tumor promotion [9,10] . The mechanism involved might associate with the inhibition of AP-1-mediated induction of COX-2 by disrupting PKC signal transduction pathway [11] . UA could inhibit the proliferation of various cancerous cell lines by inhibiting DNA polymerase and topoisomerase [12] , inducing apoptosis through proteolytic activation of caspase-3 and/ or other similar caspases [3] . It was also reported that UA has anti-invasion and anti-metastasis activity by inhibiting nuclear factor-kappa B (NF-κB) activation, down-regulating COX-2 and matrix metalloproteinase 9 [13] . Besides its cytotoxicity to hepatoma, it was reported that UA can exert its hepatoprotective action in mice and rats [14][15][16] . The reason for the phenomenon is worth studying. Moreover, the growth inhibitory activity of UA on hepatocellular carcinoma in vivo is still unknown.In the present study, UA and its six derivatives isolated from the bark of Aralia decaisneana were used to treat rheumatism, lumbago, hepatitis, nephritis, and diabetes mellitus in Chinese folk medicine. The cytotoxicity of these UA compounds to HepG2, its drug-resistance strain R-HepG2 and primarily cultured normal mouse and rat hepatocytes were examined in order to find their antihepatoma efficiency on both parental and drug-resistant hepatocellular carcinoma as well as their low toxicity. LIVER CANCER Anti-hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana Abstract AIM:To investigate the anti-tumor activity of ursolic acid (UA) and its derivatives isolated from Aralia decaisneana on hepatocellular carcinoma both in vitro and in vivo . METHODS:In vivo cytotoxicity was first screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, DNA ladder, flow cytometry analysis, Western blot and real time PCR were employed to elucidate the cytotoxic mechanism of UA. Implanted mouse hepatoma H22 was used to evaluate the growth inhibitory effect of UA in vivo .RESULTS: UA could signifi...

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Section: R : T G a T G T G G A G G T T C A G C A C C 1 9 7 B P ) A mentioning

confidence: 99%

Anti-hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana

Tian

Geng²,

Zheng³

et al. 2006

WJG

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“…The protective action of these agents is explained as a combination of various proposed mechanisms involving molecular association with carcinogens, the modulation of enzymes involved in biotransformation reactions (such as cytochrome p450 and glutathione S-transferase), anti-oxidant, anti-inflammatory, antiangiogenesis, and immunomodulatory actions, apoptosis induction, cell-cycle arrest, cell-differentiation induction, antimicrobial effects, tyrosine kinase inhibition, and others (Akiyama et al 1987;Chen et al 2003;Foti et al 2005;Moon et al 2006;Rufer and Kulling 2006;Chodon et al 2007;Choi and Kim 2008;Naithani et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

et al. 2012

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Isoflavones are phenolic compounds widely distributed in plants and found in a high percentage in soybeans. They have important biological properties and are regarded as potential chemopreventive agents. The aim of this study was to verify the preventive effect of two soy isoflavones (genistein and daidzein) by a micronucleus assay, analysis of GST activity, and real-time RT-PCR analysis of GSTa2 gene expression. Mutagens of direct (doxorubicin) and indirect (2-aminoanthracene) DNA damage were used. Hepatoma cells (HTC) were treated with genistein or daidzein for 26 h at noncytotoxic concentrations; 10 lM when alone, and 0.1, 1.0 and 10 lM when combined with genotoxic agents. The micronucleus test demonstrated that both isoflavones alone had no genotoxic effect. Genistein showed antimutagenic effects at 10 lM with both direct and indirect DNA damage agents. On phase II enzyme regulation, the current study indicated an increase in total cytoplasmic GST activity in response to genistein and daidzein at 10 lM supplementation. However, the mRNA levels of GSTa2 isozymes were not differentially modulated by genistein or daidzein. The results point to an in vitro antimutagenic activity of genistein against direct and indirect DNA damage-induced mutagenicity.

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“…Nutr., 7 (2): 22-32, 2015 in the nature (Kudou et al, 1991). Chen et al (2003) and Setchell et al (2005) reported that after the soybean is consumed, the glycosidic forms of the isoflavones undergo hydrolysis due to the action of the brush border and bacterial β-glucosidases to remove the sugar moiety, the aglycone form is then either absorbed or undergoes further metabolism by intestinal bacteria in the large bowel . Devi et al (2009) studied the functional attributes of soybean seeds and products, with reference to isoflavone content and antioxidant activity.…”

Section: Soybean and Health Benefitmentioning

confidence: 99%

Nutraceutical Potential of Soybean: Review

Tidke¹,

Ramakrishn²,

Kiran³

et al. 2015

Asian J. of Clinical Nutrition

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Soybean has received the status as one of the most important edible plants known for its seeds as a source of protein, oil and nutraceuticals. The content of the isoflavones and folic acid has made it a versatile crop for health food applications. The utility of soybean crop for food applications is described. The soybean proteins are gaining importance as a vegetable source for protein based products, with abundance quantity of essential amino acids. Its content of quality fats and PUFA are also important from nutraceutical point of view. The fermented soybean meal is also a good source of nutritionally rich tofu. In addition the biological properties of isoflavones and folic acid add value to the formulations which has been described here. Patents filed for innovations in products and processes have gained popularity and holds promise for the future of soybean industry.

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Inhibitory actions of genistein in human breast cancer (MCF-7) cells

Cited by 110 publications

References 29 publications

Anti-hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana

Anti-hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana

Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

Nutraceutical Potential of Soybean: Review

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