Inhibitory Activities of Compounds from the Marine Actinomycete <i>Williamsia</i> sp. MCCC 1A11233 Variant on IgE-Mediated Mast Cells and Passive Cutaneous Anaphylaxis

Gao, Yuanyuan; Liu, Qingmei; Liu, Bo; Xie, Conghua; Cao, Min‐Jie; Yang, Xian‐Wen

doi:10.1021/acs.jafc.7b04314

Cited by 16 publications

(8 citation statements)

References 52 publications

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“…This result prompted us to tentatively characterize fungal extract metabolites using high-resolution LC-MS analysis [ 37 ]. LC-MS analysis revealed the presence of numerous metabolites that were reported to have antimicrobial [ 38 , 39 ], anti-diabetic [ 40 ], anti-haemolytic [ 41 ], anti-inflammatory [ 42 , 43 ], anti-obesity [ 44 ], and cytotoxic activities [ 45 ] in both fungi. GC-MS analysis also proved effective in recovering already characterized VOCs with antimicrobial [ 46 ], anti-haemolytic [ 42 ], anti-inflammatory [ 47 ], anti-obesity [ 48 ] and cytotoxic activities [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites

Mefteh

Daoud

Bouket

et al. 2018

IJMS

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Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography–High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography–Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.

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Section: Discussionmentioning

confidence: 99%

Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites

Mefteh

Daoud

Bouket

et al. 2018

IJMS

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“…The aim of the present study was to investigate how tozasertib affects mast cell activation in vitro and allergic responses in vivo. We assessed mast cell activation by quantitating histamine and β‐hexosaminidase release from IgE/antigen (Ag)‐stimulated rat basophilic leukaemia cells (RBLs) and bone marrow‐derived mast cells (BMMCs) and in phorbol‐12‐myristate 13‐acetate plus calcium ionophore (PMACI)‐stimulated human LAD2 mast cells (Gao et al, 2017). These stimuli were used to induce cell degranulation in activated mast cells as an in vitro model of activated mast cells (Yamada et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Aurora kinase inhibitor tozasertib suppresses mast cell activation in vitro and in vivo

Zhang

Sun

et al. 2020

British J Pharmacology

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Background and Purpose: Mast cells are important in allergic reactions. Here, we assessed the anti-allergic effects of the anti-cancer drug tozasertib specifically regarding regulatory effects on mast cell activation. Experimental Approach: Tozasertib effects on mast cell degranulation were determined by measuring β-hexosaminidase and histamine release and by assessing morphological changes in RBL-2H3 and mouse bone marrow-derived mast cells (BMMCs) stimulated with mouse anti-dinitrophenyl (DNP)-IgE/DNP-human serum albumin or human LAD2 cells activated with phorbol-12-myristate 13-acetate plus calcium ionophore (PMACI). Western blots were performed to detect the expression of molecules involved in NF-κB, MAPK, and Aurora kinase signalling. in vivo antiallergic effects of tozasertib were determined in the murine IgE-mediated passive cutaneous anaphylaxis (PCA) and ovalbumin (OVA)-induced active systemic anaphylaxis (ASA) models. Key Results: Tozasertib treatment decreased high-affinity IgE receptor (FcεRI) or PMACI-mediated degranulation in RBL-2H3 cells and in BMMCs or LAD2 cells as shown by β-hexosaminidase or histamine levels. Similarly, tozasertib prevented morphological changes in mast cells, such as particle release and F-actin reorganization. In addition, tozasertib markedly decreased expression of phosphorylated (p)-NF-κB p65, p-Erk1/2, p-p38, and p-Aurora A/B, indicating that tozasertib can inhibit the signalling pathway mediating mast cell activation. Tozasertib attenuated IgE/Ag-induced PCA dose-dependently, as shown by reduced Evans blue staining. Similarly, tozasertib reduced body temperature levels and serum histamine levels in OVAchallenged ASA mice. Conclusion and Implications:The Aurora kinase inhibitor tozasertib suppressed mast cell activation in vitro and in vivo. Tozasertib may be a potential drug, targeting mast cell activation, to treat allergic diseases or mastocytosis.

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“…Gao and colleagues determined significant effects of several peptides (199) isolated from Indian Ocean deep (1654 m) sediment-derived actinomycete Williamsia sp. MCCC 1A11233 on both IgE-sensitized RBL-2H3 mast cell histamine and proinflammatory cytokine release, and a murine model of passive cutaneous anaphylaxis, thus observing that "the three compounds have the potential to prevent or treat IgE-sensitized allergic disorders" [216].…”

Section: Marine Compounds With Activity On the Immune Systemmentioning

confidence: 99%

“…As shown in Table 2 and Figure 2, in 2016-2017, the preclinical nervous system pharmacology of marine compounds (206)(207)(208)(209)(210)(211)(212)(213)(214)(215)(216)(217)(218)(219)(220)(221)(222)(223)(224)(225) reported several mechanisms of action involving potassium (K + ) channels, nicotinic acetylcholine, calcium (Ca 2+ ) and serotonin receptors, as well as in vivo models of antinociception and neuroprotection.…”

Section: Marine Compounds Affecting the Nervous Systemmentioning

confidence: 99%

Marine Pharmacology in 2016–2017: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

et al. 2021

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The review of the 2016–2017 marine pharmacology literature was prepared in a manner similar as the 10 prior reviews of this series. Preclinical marine pharmacology research during 2016–2017 assessed 313 marine compounds with novel pharmacology reported by a growing number of investigators from 54 countries. The peer-reviewed literature reported antibacterial, antifungal, antiprotozoal, antituberculosis, and antiviral activities for 123 marine natural products, 111 marine compounds with antidiabetic and anti-inflammatory activities as well as affecting the immune and nervous system, while in contrast 79 marine compounds displayed miscellaneous mechanisms of action which upon further investigation may contribute to several pharmacological classes. Therefore, in 2016–2017, the preclinical marine natural product pharmacology pipeline generated both novel pharmacology as well as potentially new lead compounds for the growing clinical marine pharmaceutical pipeline, and thus sustained with its contributions the global research for novel and effective therapeutic strategies for multiple disease categories.

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Inhibitory Activities of Compounds from the Marine Actinomycete Williamsia sp. MCCC 1A11233 Variant on IgE-Mediated Mast Cells and Passive Cutaneous Anaphylaxis

Cited by 16 publications

References 52 publications

Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites

Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites

Aurora kinase inhibitor tozasertib suppresses mast cell activation in vitro and in vivo

Marine Pharmacology in 2016–2017: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis and Antiviral Activities; Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action

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